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Biochemical, Antioxidant Properties and Antimicrobial Activity of Steno-Endemic Origanum onites

Origanum onites (Lamiaceae) is an Eastern Mediterranean plant that is widely used in Turkish traditional medicine. This study aimed to investigate the biochemical composition, antimicrobial activity, and antioxidant potential of O. onites. In this study, the biochemical composition of the O. onites...

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Autores principales: Canli, Kerem, Bozyel, Mustafa Eray, Turu, Dilay, Benek, Atakan, Simsek, Ozcan, Altuner, Ergin Murat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10457892/
https://www.ncbi.nlm.nih.gov/pubmed/37630547
http://dx.doi.org/10.3390/microorganisms11081987
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author Canli, Kerem
Bozyel, Mustafa Eray
Turu, Dilay
Benek, Atakan
Simsek, Ozcan
Altuner, Ergin Murat
author_facet Canli, Kerem
Bozyel, Mustafa Eray
Turu, Dilay
Benek, Atakan
Simsek, Ozcan
Altuner, Ergin Murat
author_sort Canli, Kerem
collection PubMed
description Origanum onites (Lamiaceae) is an Eastern Mediterranean plant that is widely used in Turkish traditional medicine. This study aimed to investigate the biochemical composition, antimicrobial activity, and antioxidant potential of O. onites. In this study, the biochemical composition of the O. onites ethanol extract (OOEt) was analyzed using GC-MS. The antimicrobial activity was investigated using a disk diffusion test and determining minimum inhibitory concentrations (MIC) against 30 microorganism strains, including 28 bacteria (some multidrug-resistant) and 2 fungi. Additionally, the antioxidant activity was evaluated using the DPPH method. The main component identified was carvacrol. OOEt demonstrated antimicrobial activity against a wide range of tested microorganism strains. OOEt displayed the highest activity against E. faecium (a Gram-positive bacterium) at 100 µL with a 52 mm inhibition zone. Additionally, P. aeruginosa DSMZ 50071 and P. fluorescens P1, which are Gram-negative bacteria, were the most sensitive strains with a 24 mm inhibition zone in 100 µL of OOEt. The data obtained from A. baumannii (a multidrug-resistant strain) is particularly striking, as higher activity was observed compared to all positive controls. All tested fungal strains showed more effective results than positive controls. The antioxidant activity of OOEt was found to be stronger than that of the positive control, ascorbic acid. This study determined that O. onites has significant antimicrobial and antioxidant potential.
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spelling pubmed-104578922023-08-27 Biochemical, Antioxidant Properties and Antimicrobial Activity of Steno-Endemic Origanum onites Canli, Kerem Bozyel, Mustafa Eray Turu, Dilay Benek, Atakan Simsek, Ozcan Altuner, Ergin Murat Microorganisms Article Origanum onites (Lamiaceae) is an Eastern Mediterranean plant that is widely used in Turkish traditional medicine. This study aimed to investigate the biochemical composition, antimicrobial activity, and antioxidant potential of O. onites. In this study, the biochemical composition of the O. onites ethanol extract (OOEt) was analyzed using GC-MS. The antimicrobial activity was investigated using a disk diffusion test and determining minimum inhibitory concentrations (MIC) against 30 microorganism strains, including 28 bacteria (some multidrug-resistant) and 2 fungi. Additionally, the antioxidant activity was evaluated using the DPPH method. The main component identified was carvacrol. OOEt demonstrated antimicrobial activity against a wide range of tested microorganism strains. OOEt displayed the highest activity against E. faecium (a Gram-positive bacterium) at 100 µL with a 52 mm inhibition zone. Additionally, P. aeruginosa DSMZ 50071 and P. fluorescens P1, which are Gram-negative bacteria, were the most sensitive strains with a 24 mm inhibition zone in 100 µL of OOEt. The data obtained from A. baumannii (a multidrug-resistant strain) is particularly striking, as higher activity was observed compared to all positive controls. All tested fungal strains showed more effective results than positive controls. The antioxidant activity of OOEt was found to be stronger than that of the positive control, ascorbic acid. This study determined that O. onites has significant antimicrobial and antioxidant potential. MDPI 2023-08-02 /pmc/articles/PMC10457892/ /pubmed/37630547 http://dx.doi.org/10.3390/microorganisms11081987 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Canli, Kerem
Bozyel, Mustafa Eray
Turu, Dilay
Benek, Atakan
Simsek, Ozcan
Altuner, Ergin Murat
Biochemical, Antioxidant Properties and Antimicrobial Activity of Steno-Endemic Origanum onites
title Biochemical, Antioxidant Properties and Antimicrobial Activity of Steno-Endemic Origanum onites
title_full Biochemical, Antioxidant Properties and Antimicrobial Activity of Steno-Endemic Origanum onites
title_fullStr Biochemical, Antioxidant Properties and Antimicrobial Activity of Steno-Endemic Origanum onites
title_full_unstemmed Biochemical, Antioxidant Properties and Antimicrobial Activity of Steno-Endemic Origanum onites
title_short Biochemical, Antioxidant Properties and Antimicrobial Activity of Steno-Endemic Origanum onites
title_sort biochemical, antioxidant properties and antimicrobial activity of steno-endemic origanum onites
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10457892/
https://www.ncbi.nlm.nih.gov/pubmed/37630547
http://dx.doi.org/10.3390/microorganisms11081987
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