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VP3 Phage Combined with High Salt Promotes the Lysis of Biofilm-Associated Vibrio cholerae
Cholera, caused by pathogenic Vibrio cholerae, poses a significant public health risk through water and food transmission. Biofilm-associated V. cholerae plays a crucial role in seasonal cholera outbreaks as both a reservoir in aquatic environments and a direct source of human infection. Although VP...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10458087/ https://www.ncbi.nlm.nih.gov/pubmed/37631982 http://dx.doi.org/10.3390/v15081639 |
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author | Li, Xu Li, Xiaorui Zhang, Huayao Kan, Biao Fan, Fenxia |
author_facet | Li, Xu Li, Xiaorui Zhang, Huayao Kan, Biao Fan, Fenxia |
author_sort | Li, Xu |
collection | PubMed |
description | Cholera, caused by pathogenic Vibrio cholerae, poses a significant public health risk through water and food transmission. Biofilm-associated V. cholerae plays a crucial role in seasonal cholera outbreaks as both a reservoir in aquatic environments and a direct source of human infection. Although VP3, a lytic phage, shows promise in eliminating planktonic V. cholerae from the aquatic environment, its effectiveness against biofilm-associated V. cholerae is limited. To address this limitation, our proposed approach aims to enhance the efficacy of VP3 in eliminating biofilm-associated V. cholerae by augmenting the availability of phage receptors on the surface of Vibrio cholerae. TolC is a receptor of VP3 and a salt efflux pump present in many bacteria. In this study, we employed NaCl as an enhancer to stimulate TolC expression and observed a significant enhancement of TolC expression in both planktonic and biofilm cells of V. cholerae. This enhancement led to improved adsorption of VP3. Importantly, our findings provide strong evidence that high salt concentrations combined with VP3 significantly improve the elimination of biofilm-associated V. cholerae. This approach offers a potential strategy to eliminate biofilm-formation bacteria by enhancing phage–host interaction. |
format | Online Article Text |
id | pubmed-10458087 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104580872023-08-27 VP3 Phage Combined with High Salt Promotes the Lysis of Biofilm-Associated Vibrio cholerae Li, Xu Li, Xiaorui Zhang, Huayao Kan, Biao Fan, Fenxia Viruses Article Cholera, caused by pathogenic Vibrio cholerae, poses a significant public health risk through water and food transmission. Biofilm-associated V. cholerae plays a crucial role in seasonal cholera outbreaks as both a reservoir in aquatic environments and a direct source of human infection. Although VP3, a lytic phage, shows promise in eliminating planktonic V. cholerae from the aquatic environment, its effectiveness against biofilm-associated V. cholerae is limited. To address this limitation, our proposed approach aims to enhance the efficacy of VP3 in eliminating biofilm-associated V. cholerae by augmenting the availability of phage receptors on the surface of Vibrio cholerae. TolC is a receptor of VP3 and a salt efflux pump present in many bacteria. In this study, we employed NaCl as an enhancer to stimulate TolC expression and observed a significant enhancement of TolC expression in both planktonic and biofilm cells of V. cholerae. This enhancement led to improved adsorption of VP3. Importantly, our findings provide strong evidence that high salt concentrations combined with VP3 significantly improve the elimination of biofilm-associated V. cholerae. This approach offers a potential strategy to eliminate biofilm-formation bacteria by enhancing phage–host interaction. MDPI 2023-07-27 /pmc/articles/PMC10458087/ /pubmed/37631982 http://dx.doi.org/10.3390/v15081639 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Li, Xu Li, Xiaorui Zhang, Huayao Kan, Biao Fan, Fenxia VP3 Phage Combined with High Salt Promotes the Lysis of Biofilm-Associated Vibrio cholerae |
title | VP3 Phage Combined with High Salt Promotes the Lysis of Biofilm-Associated Vibrio cholerae |
title_full | VP3 Phage Combined with High Salt Promotes the Lysis of Biofilm-Associated Vibrio cholerae |
title_fullStr | VP3 Phage Combined with High Salt Promotes the Lysis of Biofilm-Associated Vibrio cholerae |
title_full_unstemmed | VP3 Phage Combined with High Salt Promotes the Lysis of Biofilm-Associated Vibrio cholerae |
title_short | VP3 Phage Combined with High Salt Promotes the Lysis of Biofilm-Associated Vibrio cholerae |
title_sort | vp3 phage combined with high salt promotes the lysis of biofilm-associated vibrio cholerae |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10458087/ https://www.ncbi.nlm.nih.gov/pubmed/37631982 http://dx.doi.org/10.3390/v15081639 |
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