Cargando…

VP3 Phage Combined with High Salt Promotes the Lysis of Biofilm-Associated Vibrio cholerae

Cholera, caused by pathogenic Vibrio cholerae, poses a significant public health risk through water and food transmission. Biofilm-associated V. cholerae plays a crucial role in seasonal cholera outbreaks as both a reservoir in aquatic environments and a direct source of human infection. Although VP...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Xu, Li, Xiaorui, Zhang, Huayao, Kan, Biao, Fan, Fenxia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10458087/
https://www.ncbi.nlm.nih.gov/pubmed/37631982
http://dx.doi.org/10.3390/v15081639
_version_ 1785097081143164928
author Li, Xu
Li, Xiaorui
Zhang, Huayao
Kan, Biao
Fan, Fenxia
author_facet Li, Xu
Li, Xiaorui
Zhang, Huayao
Kan, Biao
Fan, Fenxia
author_sort Li, Xu
collection PubMed
description Cholera, caused by pathogenic Vibrio cholerae, poses a significant public health risk through water and food transmission. Biofilm-associated V. cholerae plays a crucial role in seasonal cholera outbreaks as both a reservoir in aquatic environments and a direct source of human infection. Although VP3, a lytic phage, shows promise in eliminating planktonic V. cholerae from the aquatic environment, its effectiveness against biofilm-associated V. cholerae is limited. To address this limitation, our proposed approach aims to enhance the efficacy of VP3 in eliminating biofilm-associated V. cholerae by augmenting the availability of phage receptors on the surface of Vibrio cholerae. TolC is a receptor of VP3 and a salt efflux pump present in many bacteria. In this study, we employed NaCl as an enhancer to stimulate TolC expression and observed a significant enhancement of TolC expression in both planktonic and biofilm cells of V. cholerae. This enhancement led to improved adsorption of VP3. Importantly, our findings provide strong evidence that high salt concentrations combined with VP3 significantly improve the elimination of biofilm-associated V. cholerae. This approach offers a potential strategy to eliminate biofilm-formation bacteria by enhancing phage–host interaction.
format Online
Article
Text
id pubmed-10458087
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-104580872023-08-27 VP3 Phage Combined with High Salt Promotes the Lysis of Biofilm-Associated Vibrio cholerae Li, Xu Li, Xiaorui Zhang, Huayao Kan, Biao Fan, Fenxia Viruses Article Cholera, caused by pathogenic Vibrio cholerae, poses a significant public health risk through water and food transmission. Biofilm-associated V. cholerae plays a crucial role in seasonal cholera outbreaks as both a reservoir in aquatic environments and a direct source of human infection. Although VP3, a lytic phage, shows promise in eliminating planktonic V. cholerae from the aquatic environment, its effectiveness against biofilm-associated V. cholerae is limited. To address this limitation, our proposed approach aims to enhance the efficacy of VP3 in eliminating biofilm-associated V. cholerae by augmenting the availability of phage receptors on the surface of Vibrio cholerae. TolC is a receptor of VP3 and a salt efflux pump present in many bacteria. In this study, we employed NaCl as an enhancer to stimulate TolC expression and observed a significant enhancement of TolC expression in both planktonic and biofilm cells of V. cholerae. This enhancement led to improved adsorption of VP3. Importantly, our findings provide strong evidence that high salt concentrations combined with VP3 significantly improve the elimination of biofilm-associated V. cholerae. This approach offers a potential strategy to eliminate biofilm-formation bacteria by enhancing phage–host interaction. MDPI 2023-07-27 /pmc/articles/PMC10458087/ /pubmed/37631982 http://dx.doi.org/10.3390/v15081639 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Xu
Li, Xiaorui
Zhang, Huayao
Kan, Biao
Fan, Fenxia
VP3 Phage Combined with High Salt Promotes the Lysis of Biofilm-Associated Vibrio cholerae
title VP3 Phage Combined with High Salt Promotes the Lysis of Biofilm-Associated Vibrio cholerae
title_full VP3 Phage Combined with High Salt Promotes the Lysis of Biofilm-Associated Vibrio cholerae
title_fullStr VP3 Phage Combined with High Salt Promotes the Lysis of Biofilm-Associated Vibrio cholerae
title_full_unstemmed VP3 Phage Combined with High Salt Promotes the Lysis of Biofilm-Associated Vibrio cholerae
title_short VP3 Phage Combined with High Salt Promotes the Lysis of Biofilm-Associated Vibrio cholerae
title_sort vp3 phage combined with high salt promotes the lysis of biofilm-associated vibrio cholerae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10458087/
https://www.ncbi.nlm.nih.gov/pubmed/37631982
http://dx.doi.org/10.3390/v15081639
work_keys_str_mv AT lixu vp3phagecombinedwithhighsaltpromotesthelysisofbiofilmassociatedvibriocholerae
AT lixiaorui vp3phagecombinedwithhighsaltpromotesthelysisofbiofilmassociatedvibriocholerae
AT zhanghuayao vp3phagecombinedwithhighsaltpromotesthelysisofbiofilmassociatedvibriocholerae
AT kanbiao vp3phagecombinedwithhighsaltpromotesthelysisofbiofilmassociatedvibriocholerae
AT fanfenxia vp3phagecombinedwithhighsaltpromotesthelysisofbiofilmassociatedvibriocholerae