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Rapid Identification of Nontuberculous Mycobacterium Species from Respiratory Specimens Using Nucleotide MALDI-TOF MS
We performed a prospective study to evaluate the diagnostic accuracy of nucleotide matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) in identifying nontuberculous mycobacterium (NTM) from clinical respiratory samples. A total of 175 eligible patients were pr...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10458091/ https://www.ncbi.nlm.nih.gov/pubmed/37630537 http://dx.doi.org/10.3390/microorganisms11081975 |
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author | Yao, Lan Gui, Xuwei Wu, Xiaocui Yang, Jinghui Fang, Yong Sun, Qin Gu, Jin Sha, Wei |
author_facet | Yao, Lan Gui, Xuwei Wu, Xiaocui Yang, Jinghui Fang, Yong Sun, Qin Gu, Jin Sha, Wei |
author_sort | Yao, Lan |
collection | PubMed |
description | We performed a prospective study to evaluate the diagnostic accuracy of nucleotide matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) in identifying nontuberculous mycobacterium (NTM) from clinical respiratory samples. A total of 175 eligible patients were prospectively enrolled, including 108 patients diagnosed with NTM pulmonary disease (NTM-PD) and 67 control patients with other diseases. All specimens were subjected to acid-fast staining, liquid culture combined with MPT64 antigen detection, and a nucleotide MALDI-TOF MS assay. NTM cultures were also subjected to the MeltPro Myco assay for species identification. Altogether, the sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of nucleotide MALDI-TOF MS were 77.8% (95% CI: 68.6–85.0%), 92.5% (82.8–97.2%), 94.4% (86.8–97.9%), and 72.1% (61.2–81.0%), respectively; these results were not statistically different from the results of culture + MPT64 antigen testing (75.0% [65.6–82.6%], 95.5% [86.6–98.8%], 96.4% [89.2–99.1%], and 70.3% [59.7–79.2%], respectively). In the identification of NTM species, of the 84 nucleotide MALDI-TOF MS positive samples, 77 samples (91.7%) were identified at the species level. Using culture + MeltPro Myco assay as the reference standard, nucleotide MALDI-TOF MS correctly identified 77.8% (63/81) of NTM species. Our results demonstrated that the nucleotide MALDI-TOF MS assay was a rapid single-step method that provided the reliable detection of NTM and identification of NTM species. This new method had the same sensitivity and specificity as the culture + MPT64 antigen method, but was much more rapid. |
format | Online Article Text |
id | pubmed-10458091 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104580912023-08-27 Rapid Identification of Nontuberculous Mycobacterium Species from Respiratory Specimens Using Nucleotide MALDI-TOF MS Yao, Lan Gui, Xuwei Wu, Xiaocui Yang, Jinghui Fang, Yong Sun, Qin Gu, Jin Sha, Wei Microorganisms Article We performed a prospective study to evaluate the diagnostic accuracy of nucleotide matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) in identifying nontuberculous mycobacterium (NTM) from clinical respiratory samples. A total of 175 eligible patients were prospectively enrolled, including 108 patients diagnosed with NTM pulmonary disease (NTM-PD) and 67 control patients with other diseases. All specimens were subjected to acid-fast staining, liquid culture combined with MPT64 antigen detection, and a nucleotide MALDI-TOF MS assay. NTM cultures were also subjected to the MeltPro Myco assay for species identification. Altogether, the sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of nucleotide MALDI-TOF MS were 77.8% (95% CI: 68.6–85.0%), 92.5% (82.8–97.2%), 94.4% (86.8–97.9%), and 72.1% (61.2–81.0%), respectively; these results were not statistically different from the results of culture + MPT64 antigen testing (75.0% [65.6–82.6%], 95.5% [86.6–98.8%], 96.4% [89.2–99.1%], and 70.3% [59.7–79.2%], respectively). In the identification of NTM species, of the 84 nucleotide MALDI-TOF MS positive samples, 77 samples (91.7%) were identified at the species level. Using culture + MeltPro Myco assay as the reference standard, nucleotide MALDI-TOF MS correctly identified 77.8% (63/81) of NTM species. Our results demonstrated that the nucleotide MALDI-TOF MS assay was a rapid single-step method that provided the reliable detection of NTM and identification of NTM species. This new method had the same sensitivity and specificity as the culture + MPT64 antigen method, but was much more rapid. MDPI 2023-07-31 /pmc/articles/PMC10458091/ /pubmed/37630537 http://dx.doi.org/10.3390/microorganisms11081975 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yao, Lan Gui, Xuwei Wu, Xiaocui Yang, Jinghui Fang, Yong Sun, Qin Gu, Jin Sha, Wei Rapid Identification of Nontuberculous Mycobacterium Species from Respiratory Specimens Using Nucleotide MALDI-TOF MS |
title | Rapid Identification of Nontuberculous Mycobacterium Species from Respiratory Specimens Using Nucleotide MALDI-TOF MS |
title_full | Rapid Identification of Nontuberculous Mycobacterium Species from Respiratory Specimens Using Nucleotide MALDI-TOF MS |
title_fullStr | Rapid Identification of Nontuberculous Mycobacterium Species from Respiratory Specimens Using Nucleotide MALDI-TOF MS |
title_full_unstemmed | Rapid Identification of Nontuberculous Mycobacterium Species from Respiratory Specimens Using Nucleotide MALDI-TOF MS |
title_short | Rapid Identification of Nontuberculous Mycobacterium Species from Respiratory Specimens Using Nucleotide MALDI-TOF MS |
title_sort | rapid identification of nontuberculous mycobacterium species from respiratory specimens using nucleotide maldi-tof ms |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10458091/ https://www.ncbi.nlm.nih.gov/pubmed/37630537 http://dx.doi.org/10.3390/microorganisms11081975 |
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