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Therapeutic Salivary Monitoring of Perampanel in Patients with Epilepsy Using a Volumetric Absorptive Microsampling Technique

The objective of this study was to validate a novel assay using the volumetric absorptive microsampling (VAMS) technique combined with liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) for the determination of the antiseizure medication perampanel in saliva and its clinical applic...

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Autores principales: Palmisani, Michela, Tartara, Elena, Johannessen Landmark, Cecilie, Crema, Francesca, De Giorgis, Valentina, Varesio, Costanza, Fattore, Cinzia, Rota, Paola, Russo, Emilio, Franco, Valentina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10458119/
https://www.ncbi.nlm.nih.gov/pubmed/37631244
http://dx.doi.org/10.3390/pharmaceutics15082030
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author Palmisani, Michela
Tartara, Elena
Johannessen Landmark, Cecilie
Crema, Francesca
De Giorgis, Valentina
Varesio, Costanza
Fattore, Cinzia
Rota, Paola
Russo, Emilio
Franco, Valentina
author_facet Palmisani, Michela
Tartara, Elena
Johannessen Landmark, Cecilie
Crema, Francesca
De Giorgis, Valentina
Varesio, Costanza
Fattore, Cinzia
Rota, Paola
Russo, Emilio
Franco, Valentina
author_sort Palmisani, Michela
collection PubMed
description The objective of this study was to validate a novel assay using the volumetric absorptive microsampling (VAMS) technique combined with liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) for the determination of the antiseizure medication perampanel in saliva and its clinical applicability in patients with epilepsy. VAMS tips were loaded with 30 μL of saliva and dried for 60 min. Analytes were extracted with methanol. The supernatant was evaporated under a gentle stream of nitrogen and reconstituted with 60 μL of methanol. Separation and quantification were achieved on a monolithic column connected to a mass spectrometer. Calibration curves were linear between 0.5 and 300 ng/mL. Intra- and inter-day accuracy was within 85.6–103.2% and intra-day and inter-day precision did not exceed 12.1%. Perampanel was stable in samples collected by VAMS and stored under different storage conditions. The VAMS-LC-MS/MS method was validated according to internationally accepted criteria and tested in patients with epilepsy who were receiving a combination of perampanel and other antiseizure medications. The method showed adequate bioanalytical performances, holding great potential as an alternative strategy to support domiciliary TDM in patients with epilepsy treated with perampanel according to the simplicity of sample collection.
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spelling pubmed-104581192023-08-27 Therapeutic Salivary Monitoring of Perampanel in Patients with Epilepsy Using a Volumetric Absorptive Microsampling Technique Palmisani, Michela Tartara, Elena Johannessen Landmark, Cecilie Crema, Francesca De Giorgis, Valentina Varesio, Costanza Fattore, Cinzia Rota, Paola Russo, Emilio Franco, Valentina Pharmaceutics Article The objective of this study was to validate a novel assay using the volumetric absorptive microsampling (VAMS) technique combined with liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) for the determination of the antiseizure medication perampanel in saliva and its clinical applicability in patients with epilepsy. VAMS tips were loaded with 30 μL of saliva and dried for 60 min. Analytes were extracted with methanol. The supernatant was evaporated under a gentle stream of nitrogen and reconstituted with 60 μL of methanol. Separation and quantification were achieved on a monolithic column connected to a mass spectrometer. Calibration curves were linear between 0.5 and 300 ng/mL. Intra- and inter-day accuracy was within 85.6–103.2% and intra-day and inter-day precision did not exceed 12.1%. Perampanel was stable in samples collected by VAMS and stored under different storage conditions. The VAMS-LC-MS/MS method was validated according to internationally accepted criteria and tested in patients with epilepsy who were receiving a combination of perampanel and other antiseizure medications. The method showed adequate bioanalytical performances, holding great potential as an alternative strategy to support domiciliary TDM in patients with epilepsy treated with perampanel according to the simplicity of sample collection. MDPI 2023-07-27 /pmc/articles/PMC10458119/ /pubmed/37631244 http://dx.doi.org/10.3390/pharmaceutics15082030 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Palmisani, Michela
Tartara, Elena
Johannessen Landmark, Cecilie
Crema, Francesca
De Giorgis, Valentina
Varesio, Costanza
Fattore, Cinzia
Rota, Paola
Russo, Emilio
Franco, Valentina
Therapeutic Salivary Monitoring of Perampanel in Patients with Epilepsy Using a Volumetric Absorptive Microsampling Technique
title Therapeutic Salivary Monitoring of Perampanel in Patients with Epilepsy Using a Volumetric Absorptive Microsampling Technique
title_full Therapeutic Salivary Monitoring of Perampanel in Patients with Epilepsy Using a Volumetric Absorptive Microsampling Technique
title_fullStr Therapeutic Salivary Monitoring of Perampanel in Patients with Epilepsy Using a Volumetric Absorptive Microsampling Technique
title_full_unstemmed Therapeutic Salivary Monitoring of Perampanel in Patients with Epilepsy Using a Volumetric Absorptive Microsampling Technique
title_short Therapeutic Salivary Monitoring of Perampanel in Patients with Epilepsy Using a Volumetric Absorptive Microsampling Technique
title_sort therapeutic salivary monitoring of perampanel in patients with epilepsy using a volumetric absorptive microsampling technique
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10458119/
https://www.ncbi.nlm.nih.gov/pubmed/37631244
http://dx.doi.org/10.3390/pharmaceutics15082030
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