Cargando…
Design and Study of Nanoceria Modified by 5-Fluorouracil for Gel and Polymer Dermal Film Preparation
In this work we studied nanoceria (CeO(2)NPs) and nanoceria modified by 5-fluorouracil (5FU) as potential APIs. Nanoceria were synthesized by precipitation in a matrix of hydroxyethyl cellulose or hydroxypropylmethyl cellulose, using cerium (III) nitrate and meglumine. Nanoceria properties were esti...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10458209/ https://www.ncbi.nlm.nih.gov/pubmed/37630997 http://dx.doi.org/10.3390/ph16081082 |
_version_ | 1785097112567939072 |
---|---|
author | Melnikova, Nina Sheferov, Ilya Panteleev, Dmitry Emasheva, Anastasia Druzhkova, Irina Ignatova, Nadezhda Mishchenko, Tatiana Vedunova, Maria |
author_facet | Melnikova, Nina Sheferov, Ilya Panteleev, Dmitry Emasheva, Anastasia Druzhkova, Irina Ignatova, Nadezhda Mishchenko, Tatiana Vedunova, Maria |
author_sort | Melnikova, Nina |
collection | PubMed |
description | In this work we studied nanoceria (CeO(2)NPs) and nanoceria modified by 5-fluorouracil (5FU) as potential APIs. Nanoceria were synthesized by precipitation in a matrix of hydroxyethyl cellulose or hydroxypropylmethyl cellulose, using cerium (III) nitrate and meglumine. Nanoceria properties were estimated by UV, FTIR and X-ray photoelectron spectra; scanning electron and atomic force microscopy; powder X-ray diffraction patterns and energy dispersive X-ray microanalysis. The cytotoxicity of nanoceria and polymer-protected nanoparticles was evaluated using the established cell line NCTC clone 929 (C3H/An mouse, subcutaneous connective tissue, clone of L. line). The morphology and metabolic activity of nanoparticles at 10 μg∙mL(−1) of cells was not significant. In addition, the cytotoxic effects of nanoceria were assessed on two human colorectal cancer cell lines (HT29 and HCT116), murine melanoma B16 cells and normal human skin fibroblasts. An inhibitory effect was shown for HCT116 human colorectal cancer cells. The IC(50) values for pure CeO(2)NPs and CeO(2)NPs-5FU were 219.0 ± 45.6 μg∙mL(−1) and 89.2 ± 14.0 μg∙mL(−1), respectively. On the other hand, the IC(50) of 5FU in the combination of CeO(2)NPs-5FU was 2-fold higher than that of pure 5FU, amounting to 5.0 nmol∙mL(−1). New compositions of nanoceria modified by 5-fluorouracil in a polymer matrix were designed as a dermal polymer film and gel. The permeability of the components was studied using a Franz cell. |
format | Online Article Text |
id | pubmed-10458209 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104582092023-08-27 Design and Study of Nanoceria Modified by 5-Fluorouracil for Gel and Polymer Dermal Film Preparation Melnikova, Nina Sheferov, Ilya Panteleev, Dmitry Emasheva, Anastasia Druzhkova, Irina Ignatova, Nadezhda Mishchenko, Tatiana Vedunova, Maria Pharmaceuticals (Basel) Article In this work we studied nanoceria (CeO(2)NPs) and nanoceria modified by 5-fluorouracil (5FU) as potential APIs. Nanoceria were synthesized by precipitation in a matrix of hydroxyethyl cellulose or hydroxypropylmethyl cellulose, using cerium (III) nitrate and meglumine. Nanoceria properties were estimated by UV, FTIR and X-ray photoelectron spectra; scanning electron and atomic force microscopy; powder X-ray diffraction patterns and energy dispersive X-ray microanalysis. The cytotoxicity of nanoceria and polymer-protected nanoparticles was evaluated using the established cell line NCTC clone 929 (C3H/An mouse, subcutaneous connective tissue, clone of L. line). The morphology and metabolic activity of nanoparticles at 10 μg∙mL(−1) of cells was not significant. In addition, the cytotoxic effects of nanoceria were assessed on two human colorectal cancer cell lines (HT29 and HCT116), murine melanoma B16 cells and normal human skin fibroblasts. An inhibitory effect was shown for HCT116 human colorectal cancer cells. The IC(50) values for pure CeO(2)NPs and CeO(2)NPs-5FU were 219.0 ± 45.6 μg∙mL(−1) and 89.2 ± 14.0 μg∙mL(−1), respectively. On the other hand, the IC(50) of 5FU in the combination of CeO(2)NPs-5FU was 2-fold higher than that of pure 5FU, amounting to 5.0 nmol∙mL(−1). New compositions of nanoceria modified by 5-fluorouracil in a polymer matrix were designed as a dermal polymer film and gel. The permeability of the components was studied using a Franz cell. MDPI 2023-07-29 /pmc/articles/PMC10458209/ /pubmed/37630997 http://dx.doi.org/10.3390/ph16081082 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Melnikova, Nina Sheferov, Ilya Panteleev, Dmitry Emasheva, Anastasia Druzhkova, Irina Ignatova, Nadezhda Mishchenko, Tatiana Vedunova, Maria Design and Study of Nanoceria Modified by 5-Fluorouracil for Gel and Polymer Dermal Film Preparation |
title | Design and Study of Nanoceria Modified by 5-Fluorouracil for Gel and Polymer Dermal Film Preparation |
title_full | Design and Study of Nanoceria Modified by 5-Fluorouracil for Gel and Polymer Dermal Film Preparation |
title_fullStr | Design and Study of Nanoceria Modified by 5-Fluorouracil for Gel and Polymer Dermal Film Preparation |
title_full_unstemmed | Design and Study of Nanoceria Modified by 5-Fluorouracil for Gel and Polymer Dermal Film Preparation |
title_short | Design and Study of Nanoceria Modified by 5-Fluorouracil for Gel and Polymer Dermal Film Preparation |
title_sort | design and study of nanoceria modified by 5-fluorouracil for gel and polymer dermal film preparation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10458209/ https://www.ncbi.nlm.nih.gov/pubmed/37630997 http://dx.doi.org/10.3390/ph16081082 |
work_keys_str_mv | AT melnikovanina designandstudyofnanoceriamodifiedby5fluorouracilforgelandpolymerdermalfilmpreparation AT sheferovilya designandstudyofnanoceriamodifiedby5fluorouracilforgelandpolymerdermalfilmpreparation AT panteleevdmitry designandstudyofnanoceriamodifiedby5fluorouracilforgelandpolymerdermalfilmpreparation AT emashevaanastasia designandstudyofnanoceriamodifiedby5fluorouracilforgelandpolymerdermalfilmpreparation AT druzhkovairina designandstudyofnanoceriamodifiedby5fluorouracilforgelandpolymerdermalfilmpreparation AT ignatovanadezhda designandstudyofnanoceriamodifiedby5fluorouracilforgelandpolymerdermalfilmpreparation AT mishchenkotatiana designandstudyofnanoceriamodifiedby5fluorouracilforgelandpolymerdermalfilmpreparation AT vedunovamaria designandstudyofnanoceriamodifiedby5fluorouracilforgelandpolymerdermalfilmpreparation |