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Detection of Plasmid-Mediated Resistance against Colistin in Multi-Drug-Resistant Gram-Negative Bacilli Isolated from a Tertiary Hospital

The aim of this study was to determine the prevalence of plasmid-mediated colistin resistance mcr-1 to mcr-5 genes among colistin and multi-drug-resistant Gram-negative bacilli strains isolated from patients in a tertiary hospital in Toluca, Mexico. The presence of mcr genes among the 241 strains co...

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Autores principales: Galindo-Méndez, Mario, Navarrete-Salazar, Humberto, Pacheco-Vásquez, Reinaldo, Quintas-de la Paz, Devanhí, Baltazar-Jiménez, Isabel, Santiago-Luna, José David, Guadarrama-Monroy, Laura
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10458375/
https://www.ncbi.nlm.nih.gov/pubmed/37630556
http://dx.doi.org/10.3390/microorganisms11081996
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author Galindo-Méndez, Mario
Navarrete-Salazar, Humberto
Pacheco-Vásquez, Reinaldo
Quintas-de la Paz, Devanhí
Baltazar-Jiménez, Isabel
Santiago-Luna, José David
Guadarrama-Monroy, Laura
author_facet Galindo-Méndez, Mario
Navarrete-Salazar, Humberto
Pacheco-Vásquez, Reinaldo
Quintas-de la Paz, Devanhí
Baltazar-Jiménez, Isabel
Santiago-Luna, José David
Guadarrama-Monroy, Laura
author_sort Galindo-Méndez, Mario
collection PubMed
description The aim of this study was to determine the prevalence of plasmid-mediated colistin resistance mcr-1 to mcr-5 genes among colistin and multi-drug-resistant Gram-negative bacilli strains isolated from patients in a tertiary hospital in Toluca, Mexico. The presence of mcr genes among the 241 strains collected was assessed by PCR. In the case of mcr-carrying E. coli, further PCR tests were performed to determine the presence of bla(CTX-M) and whether the strains belonged to the O25b-ST131 clone. Conjugation experiments were also carried out to assess the horizontal transmission of colistin resistance. A total of twelve strains (5.0%), of which four were E. coli; four were P. aeruginosa; three were K. pneumoniae, and one E. cloacae, were found to be resistant to colistin. Of these strains, two E. coli isolates were found to carry mcr-1, and Southern blot hybridization demonstrated its presence on an approximately 60 kb plasmid. Both mcr-1-carrying E. coli strains were found to co-express bla(CTX-M), belong to the O25b-ST131 clone, and horizontally transmit their colistin resistance. The results of this study confirm the presence of plasmid-mediated colistin resistance in hospitalized patients in Mexico and demonstrated that the multi-drug-resistant O25b-ST131 E. coli clone can acquire mcr genes and transmit such resistance traits to other bacteria.
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spelling pubmed-104583752023-08-27 Detection of Plasmid-Mediated Resistance against Colistin in Multi-Drug-Resistant Gram-Negative Bacilli Isolated from a Tertiary Hospital Galindo-Méndez, Mario Navarrete-Salazar, Humberto Pacheco-Vásquez, Reinaldo Quintas-de la Paz, Devanhí Baltazar-Jiménez, Isabel Santiago-Luna, José David Guadarrama-Monroy, Laura Microorganisms Article The aim of this study was to determine the prevalence of plasmid-mediated colistin resistance mcr-1 to mcr-5 genes among colistin and multi-drug-resistant Gram-negative bacilli strains isolated from patients in a tertiary hospital in Toluca, Mexico. The presence of mcr genes among the 241 strains collected was assessed by PCR. In the case of mcr-carrying E. coli, further PCR tests were performed to determine the presence of bla(CTX-M) and whether the strains belonged to the O25b-ST131 clone. Conjugation experiments were also carried out to assess the horizontal transmission of colistin resistance. A total of twelve strains (5.0%), of which four were E. coli; four were P. aeruginosa; three were K. pneumoniae, and one E. cloacae, were found to be resistant to colistin. Of these strains, two E. coli isolates were found to carry mcr-1, and Southern blot hybridization demonstrated its presence on an approximately 60 kb plasmid. Both mcr-1-carrying E. coli strains were found to co-express bla(CTX-M), belong to the O25b-ST131 clone, and horizontally transmit their colistin resistance. The results of this study confirm the presence of plasmid-mediated colistin resistance in hospitalized patients in Mexico and demonstrated that the multi-drug-resistant O25b-ST131 E. coli clone can acquire mcr genes and transmit such resistance traits to other bacteria. MDPI 2023-08-03 /pmc/articles/PMC10458375/ /pubmed/37630556 http://dx.doi.org/10.3390/microorganisms11081996 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Galindo-Méndez, Mario
Navarrete-Salazar, Humberto
Pacheco-Vásquez, Reinaldo
Quintas-de la Paz, Devanhí
Baltazar-Jiménez, Isabel
Santiago-Luna, José David
Guadarrama-Monroy, Laura
Detection of Plasmid-Mediated Resistance against Colistin in Multi-Drug-Resistant Gram-Negative Bacilli Isolated from a Tertiary Hospital
title Detection of Plasmid-Mediated Resistance against Colistin in Multi-Drug-Resistant Gram-Negative Bacilli Isolated from a Tertiary Hospital
title_full Detection of Plasmid-Mediated Resistance against Colistin in Multi-Drug-Resistant Gram-Negative Bacilli Isolated from a Tertiary Hospital
title_fullStr Detection of Plasmid-Mediated Resistance against Colistin in Multi-Drug-Resistant Gram-Negative Bacilli Isolated from a Tertiary Hospital
title_full_unstemmed Detection of Plasmid-Mediated Resistance against Colistin in Multi-Drug-Resistant Gram-Negative Bacilli Isolated from a Tertiary Hospital
title_short Detection of Plasmid-Mediated Resistance against Colistin in Multi-Drug-Resistant Gram-Negative Bacilli Isolated from a Tertiary Hospital
title_sort detection of plasmid-mediated resistance against colistin in multi-drug-resistant gram-negative bacilli isolated from a tertiary hospital
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10458375/
https://www.ncbi.nlm.nih.gov/pubmed/37630556
http://dx.doi.org/10.3390/microorganisms11081996
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