Cargando…
Detection of Plasmid-Mediated Resistance against Colistin in Multi-Drug-Resistant Gram-Negative Bacilli Isolated from a Tertiary Hospital
The aim of this study was to determine the prevalence of plasmid-mediated colistin resistance mcr-1 to mcr-5 genes among colistin and multi-drug-resistant Gram-negative bacilli strains isolated from patients in a tertiary hospital in Toluca, Mexico. The presence of mcr genes among the 241 strains co...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10458375/ https://www.ncbi.nlm.nih.gov/pubmed/37630556 http://dx.doi.org/10.3390/microorganisms11081996 |
_version_ | 1785097151308627968 |
---|---|
author | Galindo-Méndez, Mario Navarrete-Salazar, Humberto Pacheco-Vásquez, Reinaldo Quintas-de la Paz, Devanhí Baltazar-Jiménez, Isabel Santiago-Luna, José David Guadarrama-Monroy, Laura |
author_facet | Galindo-Méndez, Mario Navarrete-Salazar, Humberto Pacheco-Vásquez, Reinaldo Quintas-de la Paz, Devanhí Baltazar-Jiménez, Isabel Santiago-Luna, José David Guadarrama-Monroy, Laura |
author_sort | Galindo-Méndez, Mario |
collection | PubMed |
description | The aim of this study was to determine the prevalence of plasmid-mediated colistin resistance mcr-1 to mcr-5 genes among colistin and multi-drug-resistant Gram-negative bacilli strains isolated from patients in a tertiary hospital in Toluca, Mexico. The presence of mcr genes among the 241 strains collected was assessed by PCR. In the case of mcr-carrying E. coli, further PCR tests were performed to determine the presence of bla(CTX-M) and whether the strains belonged to the O25b-ST131 clone. Conjugation experiments were also carried out to assess the horizontal transmission of colistin resistance. A total of twelve strains (5.0%), of which four were E. coli; four were P. aeruginosa; three were K. pneumoniae, and one E. cloacae, were found to be resistant to colistin. Of these strains, two E. coli isolates were found to carry mcr-1, and Southern blot hybridization demonstrated its presence on an approximately 60 kb plasmid. Both mcr-1-carrying E. coli strains were found to co-express bla(CTX-M), belong to the O25b-ST131 clone, and horizontally transmit their colistin resistance. The results of this study confirm the presence of plasmid-mediated colistin resistance in hospitalized patients in Mexico and demonstrated that the multi-drug-resistant O25b-ST131 E. coli clone can acquire mcr genes and transmit such resistance traits to other bacteria. |
format | Online Article Text |
id | pubmed-10458375 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104583752023-08-27 Detection of Plasmid-Mediated Resistance against Colistin in Multi-Drug-Resistant Gram-Negative Bacilli Isolated from a Tertiary Hospital Galindo-Méndez, Mario Navarrete-Salazar, Humberto Pacheco-Vásquez, Reinaldo Quintas-de la Paz, Devanhí Baltazar-Jiménez, Isabel Santiago-Luna, José David Guadarrama-Monroy, Laura Microorganisms Article The aim of this study was to determine the prevalence of plasmid-mediated colistin resistance mcr-1 to mcr-5 genes among colistin and multi-drug-resistant Gram-negative bacilli strains isolated from patients in a tertiary hospital in Toluca, Mexico. The presence of mcr genes among the 241 strains collected was assessed by PCR. In the case of mcr-carrying E. coli, further PCR tests were performed to determine the presence of bla(CTX-M) and whether the strains belonged to the O25b-ST131 clone. Conjugation experiments were also carried out to assess the horizontal transmission of colistin resistance. A total of twelve strains (5.0%), of which four were E. coli; four were P. aeruginosa; three were K. pneumoniae, and one E. cloacae, were found to be resistant to colistin. Of these strains, two E. coli isolates were found to carry mcr-1, and Southern blot hybridization demonstrated its presence on an approximately 60 kb plasmid. Both mcr-1-carrying E. coli strains were found to co-express bla(CTX-M), belong to the O25b-ST131 clone, and horizontally transmit their colistin resistance. The results of this study confirm the presence of plasmid-mediated colistin resistance in hospitalized patients in Mexico and demonstrated that the multi-drug-resistant O25b-ST131 E. coli clone can acquire mcr genes and transmit such resistance traits to other bacteria. MDPI 2023-08-03 /pmc/articles/PMC10458375/ /pubmed/37630556 http://dx.doi.org/10.3390/microorganisms11081996 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Galindo-Méndez, Mario Navarrete-Salazar, Humberto Pacheco-Vásquez, Reinaldo Quintas-de la Paz, Devanhí Baltazar-Jiménez, Isabel Santiago-Luna, José David Guadarrama-Monroy, Laura Detection of Plasmid-Mediated Resistance against Colistin in Multi-Drug-Resistant Gram-Negative Bacilli Isolated from a Tertiary Hospital |
title | Detection of Plasmid-Mediated Resistance against Colistin in Multi-Drug-Resistant Gram-Negative Bacilli Isolated from a Tertiary Hospital |
title_full | Detection of Plasmid-Mediated Resistance against Colistin in Multi-Drug-Resistant Gram-Negative Bacilli Isolated from a Tertiary Hospital |
title_fullStr | Detection of Plasmid-Mediated Resistance against Colistin in Multi-Drug-Resistant Gram-Negative Bacilli Isolated from a Tertiary Hospital |
title_full_unstemmed | Detection of Plasmid-Mediated Resistance against Colistin in Multi-Drug-Resistant Gram-Negative Bacilli Isolated from a Tertiary Hospital |
title_short | Detection of Plasmid-Mediated Resistance against Colistin in Multi-Drug-Resistant Gram-Negative Bacilli Isolated from a Tertiary Hospital |
title_sort | detection of plasmid-mediated resistance against colistin in multi-drug-resistant gram-negative bacilli isolated from a tertiary hospital |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10458375/ https://www.ncbi.nlm.nih.gov/pubmed/37630556 http://dx.doi.org/10.3390/microorganisms11081996 |
work_keys_str_mv | AT galindomendezmario detectionofplasmidmediatedresistanceagainstcolistininmultidrugresistantgramnegativebacilliisolatedfromatertiaryhospital AT navarretesalazarhumberto detectionofplasmidmediatedresistanceagainstcolistininmultidrugresistantgramnegativebacilliisolatedfromatertiaryhospital AT pachecovasquezreinaldo detectionofplasmidmediatedresistanceagainstcolistininmultidrugresistantgramnegativebacilliisolatedfromatertiaryhospital AT quintasdelapazdevanhi detectionofplasmidmediatedresistanceagainstcolistininmultidrugresistantgramnegativebacilliisolatedfromatertiaryhospital AT baltazarjimenezisabel detectionofplasmidmediatedresistanceagainstcolistininmultidrugresistantgramnegativebacilliisolatedfromatertiaryhospital AT santiagolunajosedavid detectionofplasmidmediatedresistanceagainstcolistininmultidrugresistantgramnegativebacilliisolatedfromatertiaryhospital AT guadarramamonroylaura detectionofplasmidmediatedresistanceagainstcolistininmultidrugresistantgramnegativebacilliisolatedfromatertiaryhospital |