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Ring Stripping, Ring Cutting, and Growth Regulators Promote Phase Change and Early Flowering in Pear Seedlings

Hybrid breeding is the most important means of selecting pear (Pyrus) varieties, but a long juvenile period severely restricts the selection of new varieties. In this study, we used ‘Yuluxiang’ × ‘Akituki’ 4-year-old seedling trees to study the effects of plant growth regulators, ring stripping, and...

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Autores principales: Zhang, Xiaojie, Wu, Yueju, Wang, Xiaoming, Wang, Wenfang, Huang, Mingxia, Ma, Zitan, Peng, Jianying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10458443/
https://www.ncbi.nlm.nih.gov/pubmed/37631145
http://dx.doi.org/10.3390/plants12162933
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author Zhang, Xiaojie
Wu, Yueju
Wang, Xiaoming
Wang, Wenfang
Huang, Mingxia
Ma, Zitan
Peng, Jianying
author_facet Zhang, Xiaojie
Wu, Yueju
Wang, Xiaoming
Wang, Wenfang
Huang, Mingxia
Ma, Zitan
Peng, Jianying
author_sort Zhang, Xiaojie
collection PubMed
description Hybrid breeding is the most important means of selecting pear (Pyrus) varieties, but a long juvenile period severely restricts the selection of new varieties. In this study, we used ‘Yuluxiang’ × ‘Akituki’ 4-year-old seedling trees to study the effects of plant growth regulators, ring stripping, and ring cutting on the promotion of phase change and flowering to assist in shortening the breeding cycle. A single application of 100 mg/kg 6-BA + 1000 mg/kg PP333 was most effective in promoting phase change and flowering. This treatment effectively inhibited the growth and thickening of annual shoots, significantly increased soluble sugar and protein contents in buds, increased the ABA content by 45.41%, decreased the IAA content by 7.35%, increased the expression of the flower-promoting genes FT and LFY by 2273.41% and 1153.71%, respectively, and decreased the expression of the flower-suppressing gene TFL1 by 74.92%. The flowering plant rate increased by 23.34% compared to the control. Both ring stripping and ring cutting were effective in promoting phase change and flowering, significantly increasing the flowering rate, inflorescence number, and the number of flowering plants. For improving the flowering rate, the ring-stripping treatment had the strongest effect and effectively inhibited the growth and thickening of annual shoots, while also significantly increasing the soluble sugar and protein contents in buds, reducing the contents of IAA and GA3 by 8.73% and 50.12%, respectively, increasing the expression of FT and LFY by 80.01% and 821.14%, respectively, and reducing the expression of the flower-suppressing gene TFL1 by 59.22%. In conclusion, ring stripping, ring cutting, and spraying of 100 mg/kg 6-BA + 1000 mg/kg PP333 were effective in promoting phase change and early flowering in seedling trees.
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spelling pubmed-104584432023-08-27 Ring Stripping, Ring Cutting, and Growth Regulators Promote Phase Change and Early Flowering in Pear Seedlings Zhang, Xiaojie Wu, Yueju Wang, Xiaoming Wang, Wenfang Huang, Mingxia Ma, Zitan Peng, Jianying Plants (Basel) Article Hybrid breeding is the most important means of selecting pear (Pyrus) varieties, but a long juvenile period severely restricts the selection of new varieties. In this study, we used ‘Yuluxiang’ × ‘Akituki’ 4-year-old seedling trees to study the effects of plant growth regulators, ring stripping, and ring cutting on the promotion of phase change and flowering to assist in shortening the breeding cycle. A single application of 100 mg/kg 6-BA + 1000 mg/kg PP333 was most effective in promoting phase change and flowering. This treatment effectively inhibited the growth and thickening of annual shoots, significantly increased soluble sugar and protein contents in buds, increased the ABA content by 45.41%, decreased the IAA content by 7.35%, increased the expression of the flower-promoting genes FT and LFY by 2273.41% and 1153.71%, respectively, and decreased the expression of the flower-suppressing gene TFL1 by 74.92%. The flowering plant rate increased by 23.34% compared to the control. Both ring stripping and ring cutting were effective in promoting phase change and flowering, significantly increasing the flowering rate, inflorescence number, and the number of flowering plants. For improving the flowering rate, the ring-stripping treatment had the strongest effect and effectively inhibited the growth and thickening of annual shoots, while also significantly increasing the soluble sugar and protein contents in buds, reducing the contents of IAA and GA3 by 8.73% and 50.12%, respectively, increasing the expression of FT and LFY by 80.01% and 821.14%, respectively, and reducing the expression of the flower-suppressing gene TFL1 by 59.22%. In conclusion, ring stripping, ring cutting, and spraying of 100 mg/kg 6-BA + 1000 mg/kg PP333 were effective in promoting phase change and early flowering in seedling trees. MDPI 2023-08-14 /pmc/articles/PMC10458443/ /pubmed/37631145 http://dx.doi.org/10.3390/plants12162933 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Xiaojie
Wu, Yueju
Wang, Xiaoming
Wang, Wenfang
Huang, Mingxia
Ma, Zitan
Peng, Jianying
Ring Stripping, Ring Cutting, and Growth Regulators Promote Phase Change and Early Flowering in Pear Seedlings
title Ring Stripping, Ring Cutting, and Growth Regulators Promote Phase Change and Early Flowering in Pear Seedlings
title_full Ring Stripping, Ring Cutting, and Growth Regulators Promote Phase Change and Early Flowering in Pear Seedlings
title_fullStr Ring Stripping, Ring Cutting, and Growth Regulators Promote Phase Change and Early Flowering in Pear Seedlings
title_full_unstemmed Ring Stripping, Ring Cutting, and Growth Regulators Promote Phase Change and Early Flowering in Pear Seedlings
title_short Ring Stripping, Ring Cutting, and Growth Regulators Promote Phase Change and Early Flowering in Pear Seedlings
title_sort ring stripping, ring cutting, and growth regulators promote phase change and early flowering in pear seedlings
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10458443/
https://www.ncbi.nlm.nih.gov/pubmed/37631145
http://dx.doi.org/10.3390/plants12162933
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