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Surface Characteristics Affect the Properties of PLGA Nanoparticles as Photothermal Agents
Photothermal therapy is one of the most promising and rapidly developing fields in modern oncology due to its high efficiency, localized action, and minimal invasiveness. Polymeric nanoparticles (NPs) incorporating low molecular-weight photothermal dyes are capable of delivering therapeutic agents t...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10458446/ https://www.ncbi.nlm.nih.gov/pubmed/37630183 http://dx.doi.org/10.3390/mi14081647 |
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author | Kovalenko, Vera L. Kolesnikova, Olga A. Nikitin, Maxim P. Shipunova, Victoria O. Komedchikova, Elena N. |
author_facet | Kovalenko, Vera L. Kolesnikova, Olga A. Nikitin, Maxim P. Shipunova, Victoria O. Komedchikova, Elena N. |
author_sort | Kovalenko, Vera L. |
collection | PubMed |
description | Photothermal therapy is one of the most promising and rapidly developing fields in modern oncology due to its high efficiency, localized action, and minimal invasiveness. Polymeric nanoparticles (NPs) incorporating low molecular-weight photothermal dyes are capable of delivering therapeutic agents to the tumor site, releasing them in a controlled manner, and providing tumor treatment under external light irradiation. The nanoparticle synthesis components are critically important factors that influence the therapeutically significant characteristics of polymeric NPs. Here, we show the impact of stabilizers and solvents used for synthesis on the properties of PLGA NPs for photothermal therapy. We synthesized PLGA nanocarriers using the microemulsion method and varied the nature of the solvent and the concentration of the stabilizer—namely, chitosan oligosaccharide lactate. A phthalocyanine-based photosensitizer, which absorbs light in the NIR window, was encapsulated in the PLGA NPs. When mQ water was used as a solvent and chitosan oligosaccharide lactate was used at a concentration of 1 g/L, the PLGA NPs exhibited highly promising photothermal properties. The final composite of the nanocarriers demonstrated photoinduced cytotoxicity against EMT6/P cells under NIR laser irradiation in vitro and was suitable for bioimaging. |
format | Online Article Text |
id | pubmed-10458446 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104584462023-08-27 Surface Characteristics Affect the Properties of PLGA Nanoparticles as Photothermal Agents Kovalenko, Vera L. Kolesnikova, Olga A. Nikitin, Maxim P. Shipunova, Victoria O. Komedchikova, Elena N. Micromachines (Basel) Article Photothermal therapy is one of the most promising and rapidly developing fields in modern oncology due to its high efficiency, localized action, and minimal invasiveness. Polymeric nanoparticles (NPs) incorporating low molecular-weight photothermal dyes are capable of delivering therapeutic agents to the tumor site, releasing them in a controlled manner, and providing tumor treatment under external light irradiation. The nanoparticle synthesis components are critically important factors that influence the therapeutically significant characteristics of polymeric NPs. Here, we show the impact of stabilizers and solvents used for synthesis on the properties of PLGA NPs for photothermal therapy. We synthesized PLGA nanocarriers using the microemulsion method and varied the nature of the solvent and the concentration of the stabilizer—namely, chitosan oligosaccharide lactate. A phthalocyanine-based photosensitizer, which absorbs light in the NIR window, was encapsulated in the PLGA NPs. When mQ water was used as a solvent and chitosan oligosaccharide lactate was used at a concentration of 1 g/L, the PLGA NPs exhibited highly promising photothermal properties. The final composite of the nanocarriers demonstrated photoinduced cytotoxicity against EMT6/P cells under NIR laser irradiation in vitro and was suitable for bioimaging. MDPI 2023-08-21 /pmc/articles/PMC10458446/ /pubmed/37630183 http://dx.doi.org/10.3390/mi14081647 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kovalenko, Vera L. Kolesnikova, Olga A. Nikitin, Maxim P. Shipunova, Victoria O. Komedchikova, Elena N. Surface Characteristics Affect the Properties of PLGA Nanoparticles as Photothermal Agents |
title | Surface Characteristics Affect the Properties of PLGA Nanoparticles as Photothermal Agents |
title_full | Surface Characteristics Affect the Properties of PLGA Nanoparticles as Photothermal Agents |
title_fullStr | Surface Characteristics Affect the Properties of PLGA Nanoparticles as Photothermal Agents |
title_full_unstemmed | Surface Characteristics Affect the Properties of PLGA Nanoparticles as Photothermal Agents |
title_short | Surface Characteristics Affect the Properties of PLGA Nanoparticles as Photothermal Agents |
title_sort | surface characteristics affect the properties of plga nanoparticles as photothermal agents |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10458446/ https://www.ncbi.nlm.nih.gov/pubmed/37630183 http://dx.doi.org/10.3390/mi14081647 |
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