Cargando…
Effects of Water–Ethanol Extracts from Four Sphagnum Species on Gene Expression of Selected Enzymes in Normal Human Dermal Fibroblasts and Their Antioxidant Properties
Mosses (Bryophyta), particularly species of the genus Sphagnum, which have been used for centuries for the treatment of skin diseases and damage, are still not explored enough in terms of their use in cosmetics. The purpose of this study was to determine the antioxidant properties of water–ethanol e...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10458669/ https://www.ncbi.nlm.nih.gov/pubmed/37630991 http://dx.doi.org/10.3390/ph16081076 |
_version_ | 1785097221526519808 |
---|---|
author | Zych, Maria Urbisz, Katarzyna Kimsa-Dudek, Magdalena Kamionka, Maria Dudek, Sławomir Raczak, Barbara Klaudia Wacławek, Stanisław Chmura, Damian Kaczmarczyk-Żebrowska, Ilona Stebel, Adam |
author_facet | Zych, Maria Urbisz, Katarzyna Kimsa-Dudek, Magdalena Kamionka, Maria Dudek, Sławomir Raczak, Barbara Klaudia Wacławek, Stanisław Chmura, Damian Kaczmarczyk-Żebrowska, Ilona Stebel, Adam |
author_sort | Zych, Maria |
collection | PubMed |
description | Mosses (Bryophyta), particularly species of the genus Sphagnum, which have been used for centuries for the treatment of skin diseases and damage, are still not explored enough in terms of their use in cosmetics. The purpose of this study was to determine the antioxidant properties of water–ethanol extracts from four selected species of the genus Sphagnum (S. girgenshonii Russow, S. magellanicum Brid., S. palustre L., and S. squarrosum Crome) and their impact on the expression of genes encoding key enzymes for the functioning of the skin. In this study, the effects of Sphagnum extracts on the expression of genes encoding tyrosinase, collagenase, elastase, hyaluronidase and hyaluronic acid synthase in human dermal fibroblasts were determined for the first time in vitro. The extracts inhibited tyrosinase gene expression and showed antioxidant activity. The experiment showed an increase in the expression of some genes encoding collagenase (MMP1) or hyaluronidase (HYAL2, HYAL3 and HYAL4) and a decrease in the hyaluronan synthase (HAS1, HAS2 and HAS3) genes expression by the tested extracts. The obtained results suggest that using extracts from the tested Sphagnum species in anti-aging cosmetics does not seem beneficial. Further studies are needed to clarify their impact on the skin. |
format | Online Article Text |
id | pubmed-10458669 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104586692023-08-27 Effects of Water–Ethanol Extracts from Four Sphagnum Species on Gene Expression of Selected Enzymes in Normal Human Dermal Fibroblasts and Their Antioxidant Properties Zych, Maria Urbisz, Katarzyna Kimsa-Dudek, Magdalena Kamionka, Maria Dudek, Sławomir Raczak, Barbara Klaudia Wacławek, Stanisław Chmura, Damian Kaczmarczyk-Żebrowska, Ilona Stebel, Adam Pharmaceuticals (Basel) Article Mosses (Bryophyta), particularly species of the genus Sphagnum, which have been used for centuries for the treatment of skin diseases and damage, are still not explored enough in terms of their use in cosmetics. The purpose of this study was to determine the antioxidant properties of water–ethanol extracts from four selected species of the genus Sphagnum (S. girgenshonii Russow, S. magellanicum Brid., S. palustre L., and S. squarrosum Crome) and their impact on the expression of genes encoding key enzymes for the functioning of the skin. In this study, the effects of Sphagnum extracts on the expression of genes encoding tyrosinase, collagenase, elastase, hyaluronidase and hyaluronic acid synthase in human dermal fibroblasts were determined for the first time in vitro. The extracts inhibited tyrosinase gene expression and showed antioxidant activity. The experiment showed an increase in the expression of some genes encoding collagenase (MMP1) or hyaluronidase (HYAL2, HYAL3 and HYAL4) and a decrease in the hyaluronan synthase (HAS1, HAS2 and HAS3) genes expression by the tested extracts. The obtained results suggest that using extracts from the tested Sphagnum species in anti-aging cosmetics does not seem beneficial. Further studies are needed to clarify their impact on the skin. MDPI 2023-07-28 /pmc/articles/PMC10458669/ /pubmed/37630991 http://dx.doi.org/10.3390/ph16081076 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zych, Maria Urbisz, Katarzyna Kimsa-Dudek, Magdalena Kamionka, Maria Dudek, Sławomir Raczak, Barbara Klaudia Wacławek, Stanisław Chmura, Damian Kaczmarczyk-Żebrowska, Ilona Stebel, Adam Effects of Water–Ethanol Extracts from Four Sphagnum Species on Gene Expression of Selected Enzymes in Normal Human Dermal Fibroblasts and Their Antioxidant Properties |
title | Effects of Water–Ethanol Extracts from Four Sphagnum Species on Gene Expression of Selected Enzymes in Normal Human Dermal Fibroblasts and Their Antioxidant Properties |
title_full | Effects of Water–Ethanol Extracts from Four Sphagnum Species on Gene Expression of Selected Enzymes in Normal Human Dermal Fibroblasts and Their Antioxidant Properties |
title_fullStr | Effects of Water–Ethanol Extracts from Four Sphagnum Species on Gene Expression of Selected Enzymes in Normal Human Dermal Fibroblasts and Their Antioxidant Properties |
title_full_unstemmed | Effects of Water–Ethanol Extracts from Four Sphagnum Species on Gene Expression of Selected Enzymes in Normal Human Dermal Fibroblasts and Their Antioxidant Properties |
title_short | Effects of Water–Ethanol Extracts from Four Sphagnum Species on Gene Expression of Selected Enzymes in Normal Human Dermal Fibroblasts and Their Antioxidant Properties |
title_sort | effects of water–ethanol extracts from four sphagnum species on gene expression of selected enzymes in normal human dermal fibroblasts and their antioxidant properties |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10458669/ https://www.ncbi.nlm.nih.gov/pubmed/37630991 http://dx.doi.org/10.3390/ph16081076 |
work_keys_str_mv | AT zychmaria effectsofwaterethanolextractsfromfoursphagnumspeciesongeneexpressionofselectedenzymesinnormalhumandermalfibroblastsandtheirantioxidantproperties AT urbiszkatarzyna effectsofwaterethanolextractsfromfoursphagnumspeciesongeneexpressionofselectedenzymesinnormalhumandermalfibroblastsandtheirantioxidantproperties AT kimsadudekmagdalena effectsofwaterethanolextractsfromfoursphagnumspeciesongeneexpressionofselectedenzymesinnormalhumandermalfibroblastsandtheirantioxidantproperties AT kamionkamaria effectsofwaterethanolextractsfromfoursphagnumspeciesongeneexpressionofselectedenzymesinnormalhumandermalfibroblastsandtheirantioxidantproperties AT dudeksławomir effectsofwaterethanolextractsfromfoursphagnumspeciesongeneexpressionofselectedenzymesinnormalhumandermalfibroblastsandtheirantioxidantproperties AT raczakbarbaraklaudia effectsofwaterethanolextractsfromfoursphagnumspeciesongeneexpressionofselectedenzymesinnormalhumandermalfibroblastsandtheirantioxidantproperties AT wacławekstanisław effectsofwaterethanolextractsfromfoursphagnumspeciesongeneexpressionofselectedenzymesinnormalhumandermalfibroblastsandtheirantioxidantproperties AT chmuradamian effectsofwaterethanolextractsfromfoursphagnumspeciesongeneexpressionofselectedenzymesinnormalhumandermalfibroblastsandtheirantioxidantproperties AT kaczmarczykzebrowskailona effectsofwaterethanolextractsfromfoursphagnumspeciesongeneexpressionofselectedenzymesinnormalhumandermalfibroblastsandtheirantioxidantproperties AT stebeladam effectsofwaterethanolextractsfromfoursphagnumspeciesongeneexpressionofselectedenzymesinnormalhumandermalfibroblastsandtheirantioxidantproperties |