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Detection of Cyclomodulin CNF-1 Toxin-Producing Strains of Escherichia coli in Pig Kidneys at a Slaughterhouse

Food is often contaminated with Escherichia coli (E. coli) bacteria strains, which have been associated with different diseases, including urinary tract infections. The consumption of meat by humans is a potential route of transmission of antimicrobial resistance, and food-producing animals have bee...

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Autores principales: Herrera-Vázquez, Arturo, Arellano-Aranda, Rebeca, Hernández-Cueto, Daniel, Rodríguez-Miranda, Esmeralda, López-Briones, Sergio, Hernández-Luna, Marco Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10458685/
https://www.ncbi.nlm.nih.gov/pubmed/37630625
http://dx.doi.org/10.3390/microorganisms11082065
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author Herrera-Vázquez, Arturo
Arellano-Aranda, Rebeca
Hernández-Cueto, Daniel
Rodríguez-Miranda, Esmeralda
López-Briones, Sergio
Hernández-Luna, Marco Antonio
author_facet Herrera-Vázquez, Arturo
Arellano-Aranda, Rebeca
Hernández-Cueto, Daniel
Rodríguez-Miranda, Esmeralda
López-Briones, Sergio
Hernández-Luna, Marco Antonio
author_sort Herrera-Vázquez, Arturo
collection PubMed
description Food is often contaminated with Escherichia coli (E. coli) bacteria strains, which have been associated with different diseases, including urinary tract infections. The consumption of meat by humans is a potential route of transmission of antimicrobial resistance, and food-producing animals have been associated as a major reservoir of resistant bacterial strains. The aim of this study was to determine the presence of the E. coli strains producing the CNF-1 toxin in pig kidneys. Pig kidneys were collected from a Mexican slaughterhouse and classified according to their coloration into reddish kidneys (RK) and yellowish kidneys (YK). A tissue sample from each kidney was processed for histological analysis, the presence of E. coli was determined by conventional PCR assay, and the CNF-1 toxin was detected by both conventional PCR and Western blotting. Herein, an inflammatory cell infiltrate was found in all collected kidneys, regardless of macroscopic differences. Surprisingly, E. coli and the CNF-1 toxin were detected in all kidney samples. We clearly demonstrate contamination by CNF-1 toxin-producing E. coli in pork kidneys from a slaughterhouse, even in those without apparent damage. This suggests that pork may serve as a reservoir for pathogens, representing an important risk to human health.
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spelling pubmed-104586852023-08-27 Detection of Cyclomodulin CNF-1 Toxin-Producing Strains of Escherichia coli in Pig Kidneys at a Slaughterhouse Herrera-Vázquez, Arturo Arellano-Aranda, Rebeca Hernández-Cueto, Daniel Rodríguez-Miranda, Esmeralda López-Briones, Sergio Hernández-Luna, Marco Antonio Microorganisms Article Food is often contaminated with Escherichia coli (E. coli) bacteria strains, which have been associated with different diseases, including urinary tract infections. The consumption of meat by humans is a potential route of transmission of antimicrobial resistance, and food-producing animals have been associated as a major reservoir of resistant bacterial strains. The aim of this study was to determine the presence of the E. coli strains producing the CNF-1 toxin in pig kidneys. Pig kidneys were collected from a Mexican slaughterhouse and classified according to their coloration into reddish kidneys (RK) and yellowish kidneys (YK). A tissue sample from each kidney was processed for histological analysis, the presence of E. coli was determined by conventional PCR assay, and the CNF-1 toxin was detected by both conventional PCR and Western blotting. Herein, an inflammatory cell infiltrate was found in all collected kidneys, regardless of macroscopic differences. Surprisingly, E. coli and the CNF-1 toxin were detected in all kidney samples. We clearly demonstrate contamination by CNF-1 toxin-producing E. coli in pork kidneys from a slaughterhouse, even in those without apparent damage. This suggests that pork may serve as a reservoir for pathogens, representing an important risk to human health. MDPI 2023-08-11 /pmc/articles/PMC10458685/ /pubmed/37630625 http://dx.doi.org/10.3390/microorganisms11082065 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Herrera-Vázquez, Arturo
Arellano-Aranda, Rebeca
Hernández-Cueto, Daniel
Rodríguez-Miranda, Esmeralda
López-Briones, Sergio
Hernández-Luna, Marco Antonio
Detection of Cyclomodulin CNF-1 Toxin-Producing Strains of Escherichia coli in Pig Kidneys at a Slaughterhouse
title Detection of Cyclomodulin CNF-1 Toxin-Producing Strains of Escherichia coli in Pig Kidneys at a Slaughterhouse
title_full Detection of Cyclomodulin CNF-1 Toxin-Producing Strains of Escherichia coli in Pig Kidneys at a Slaughterhouse
title_fullStr Detection of Cyclomodulin CNF-1 Toxin-Producing Strains of Escherichia coli in Pig Kidneys at a Slaughterhouse
title_full_unstemmed Detection of Cyclomodulin CNF-1 Toxin-Producing Strains of Escherichia coli in Pig Kidneys at a Slaughterhouse
title_short Detection of Cyclomodulin CNF-1 Toxin-Producing Strains of Escherichia coli in Pig Kidneys at a Slaughterhouse
title_sort detection of cyclomodulin cnf-1 toxin-producing strains of escherichia coli in pig kidneys at a slaughterhouse
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10458685/
https://www.ncbi.nlm.nih.gov/pubmed/37630625
http://dx.doi.org/10.3390/microorganisms11082065
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