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Transcriptome Analysis Identifies the Crosstalk between Dendritic and Natural Killer Cells in Human Cutaneous Leishmaniasis

Skin ulcers of cutaneous leishmaniasis (CL) are characterized by a localized inflammatory response mediated by innate and adaptive immune cells, including dendritic cells (DC) and natural killer (NK) cells. Bidirectional interactions between DCs and NK cells contribute to tailor leishmaniasis outcom...

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Autores principales: Nunes, Sara, Tibúrcio, Rafael, Bonyek-Silva, Icaro, Oliveira, Pablo Rafael, Khouri, Ricardo, Boaventura, Viviane, Barral, Aldina, Brodskyn, Cláudia, Tavares, Natalia Machado
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10459107/
https://www.ncbi.nlm.nih.gov/pubmed/37630497
http://dx.doi.org/10.3390/microorganisms11081937
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author Nunes, Sara
Tibúrcio, Rafael
Bonyek-Silva, Icaro
Oliveira, Pablo Rafael
Khouri, Ricardo
Boaventura, Viviane
Barral, Aldina
Brodskyn, Cláudia
Tavares, Natalia Machado
author_facet Nunes, Sara
Tibúrcio, Rafael
Bonyek-Silva, Icaro
Oliveira, Pablo Rafael
Khouri, Ricardo
Boaventura, Viviane
Barral, Aldina
Brodskyn, Cláudia
Tavares, Natalia Machado
author_sort Nunes, Sara
collection PubMed
description Skin ulcers of cutaneous leishmaniasis (CL) are characterized by a localized inflammatory response mediated by innate and adaptive immune cells, including dendritic cells (DC) and natural killer (NK) cells. Bidirectional interactions between DCs and NK cells contribute to tailor leishmaniasis outcome. Despite advances in the Leishmania biology field in recent decades, the mechanisms involved in DC/NK-mediated control of Leishmania sp. pathogenesis as well as the cellular and molecular players involved in such interaction remain unclear. The present study sought to investigate canonical pathways associated with CL arising from Leishmania braziliensis infection. Initially, two publicly available microarray datasets of skin biopsies from active CL lesions were analyzed, and five pathways were identified using differentially expressed genes. The “Crosstalk between DCs and NK cells” pathway was notable due to a high number of modulated genes. The molecules significantly involved in this pathway were identified, and our findings were validated in newly obtained CL biopsies. We found increased expression of TLR4, TNFRSF1B, IL-15, IL-6, CD40, CCR7, TNF and IFNG, confirming the analysis of publicly available datasets. These findings reveal the “crosstalk between DCs and NK cells” as a potential pathway to be further explored in the pathogenesis of CL, especially the expression of CCR7, which is correlated with lesion development.
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spelling pubmed-104591072023-08-27 Transcriptome Analysis Identifies the Crosstalk between Dendritic and Natural Killer Cells in Human Cutaneous Leishmaniasis Nunes, Sara Tibúrcio, Rafael Bonyek-Silva, Icaro Oliveira, Pablo Rafael Khouri, Ricardo Boaventura, Viviane Barral, Aldina Brodskyn, Cláudia Tavares, Natalia Machado Microorganisms Article Skin ulcers of cutaneous leishmaniasis (CL) are characterized by a localized inflammatory response mediated by innate and adaptive immune cells, including dendritic cells (DC) and natural killer (NK) cells. Bidirectional interactions between DCs and NK cells contribute to tailor leishmaniasis outcome. Despite advances in the Leishmania biology field in recent decades, the mechanisms involved in DC/NK-mediated control of Leishmania sp. pathogenesis as well as the cellular and molecular players involved in such interaction remain unclear. The present study sought to investigate canonical pathways associated with CL arising from Leishmania braziliensis infection. Initially, two publicly available microarray datasets of skin biopsies from active CL lesions were analyzed, and five pathways were identified using differentially expressed genes. The “Crosstalk between DCs and NK cells” pathway was notable due to a high number of modulated genes. The molecules significantly involved in this pathway were identified, and our findings were validated in newly obtained CL biopsies. We found increased expression of TLR4, TNFRSF1B, IL-15, IL-6, CD40, CCR7, TNF and IFNG, confirming the analysis of publicly available datasets. These findings reveal the “crosstalk between DCs and NK cells” as a potential pathway to be further explored in the pathogenesis of CL, especially the expression of CCR7, which is correlated with lesion development. MDPI 2023-07-29 /pmc/articles/PMC10459107/ /pubmed/37630497 http://dx.doi.org/10.3390/microorganisms11081937 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nunes, Sara
Tibúrcio, Rafael
Bonyek-Silva, Icaro
Oliveira, Pablo Rafael
Khouri, Ricardo
Boaventura, Viviane
Barral, Aldina
Brodskyn, Cláudia
Tavares, Natalia Machado
Transcriptome Analysis Identifies the Crosstalk between Dendritic and Natural Killer Cells in Human Cutaneous Leishmaniasis
title Transcriptome Analysis Identifies the Crosstalk between Dendritic and Natural Killer Cells in Human Cutaneous Leishmaniasis
title_full Transcriptome Analysis Identifies the Crosstalk between Dendritic and Natural Killer Cells in Human Cutaneous Leishmaniasis
title_fullStr Transcriptome Analysis Identifies the Crosstalk between Dendritic and Natural Killer Cells in Human Cutaneous Leishmaniasis
title_full_unstemmed Transcriptome Analysis Identifies the Crosstalk between Dendritic and Natural Killer Cells in Human Cutaneous Leishmaniasis
title_short Transcriptome Analysis Identifies the Crosstalk between Dendritic and Natural Killer Cells in Human Cutaneous Leishmaniasis
title_sort transcriptome analysis identifies the crosstalk between dendritic and natural killer cells in human cutaneous leishmaniasis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10459107/
https://www.ncbi.nlm.nih.gov/pubmed/37630497
http://dx.doi.org/10.3390/microorganisms11081937
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