Cargando…
Usefulness of Next-Generation Sequencing in Excluding Bovine Leukemia Virus as a Cause of Adult Camel Leukosis in Dromedaries
Adult camel leukosis is an emerging hematological and neoplastic disease in dromedaries. It has been hypothesized that bovine leukemia virus (BLV) or its genetic variants may be associated with adult camel leukosis. In this study, we used next-generation sequencing (NGS) to detect all possible virus...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10459180/ https://www.ncbi.nlm.nih.gov/pubmed/37623955 http://dx.doi.org/10.3390/pathogens12080995 |
_version_ | 1785097348096983040 |
---|---|
author | Wernery, Ulrich Teng, Jade L. L. Ma, Yuanchao Kinne, Joerg Yeung, Man-Lung Anas, Safna Lau, Susanna K. P. Woo, Patrick C. Y. |
author_facet | Wernery, Ulrich Teng, Jade L. L. Ma, Yuanchao Kinne, Joerg Yeung, Man-Lung Anas, Safna Lau, Susanna K. P. Woo, Patrick C. Y. |
author_sort | Wernery, Ulrich |
collection | PubMed |
description | Adult camel leukosis is an emerging hematological and neoplastic disease in dromedaries. It has been hypothesized that bovine leukemia virus (BLV) or its genetic variants may be associated with adult camel leukosis. In this study, we used next-generation sequencing (NGS) to detect all possible viruses in five lung samples from five dromedaries with histopathological evidence of adult camel leukosis and four tissue samples from two control dromedaries. A total throughput of 114.7 Gb was achieved, with an average of 12.7 Gb/sample. For each sample, all the pair-end 151-bp reads were filtered to remove rRNA sequences, bacterial genomes and redundant sequences, resulting in 1–7 Gb clean reads, of which <3% matched to viruses. The largest portion of these viral sequences was composed of bacterial phages. About 100–300 reads in each sample matched “multiple sclerosis-associated retrovirus”, but manual analysis showed that they were only repetitive sequences commonly present in mammalian genomes. All viral reads were also extracted for analysis, confirming that no BLV or its genetic variants or any other virus was detected in the nine tissue samples. NGS is not only useful for detecting microorganisms associated with infectious diseases, but also important for excluding an infective cause in scenarios where such a possibility is suspected. |
format | Online Article Text |
id | pubmed-10459180 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104591802023-08-27 Usefulness of Next-Generation Sequencing in Excluding Bovine Leukemia Virus as a Cause of Adult Camel Leukosis in Dromedaries Wernery, Ulrich Teng, Jade L. L. Ma, Yuanchao Kinne, Joerg Yeung, Man-Lung Anas, Safna Lau, Susanna K. P. Woo, Patrick C. Y. Pathogens Brief Report Adult camel leukosis is an emerging hematological and neoplastic disease in dromedaries. It has been hypothesized that bovine leukemia virus (BLV) or its genetic variants may be associated with adult camel leukosis. In this study, we used next-generation sequencing (NGS) to detect all possible viruses in five lung samples from five dromedaries with histopathological evidence of adult camel leukosis and four tissue samples from two control dromedaries. A total throughput of 114.7 Gb was achieved, with an average of 12.7 Gb/sample. For each sample, all the pair-end 151-bp reads were filtered to remove rRNA sequences, bacterial genomes and redundant sequences, resulting in 1–7 Gb clean reads, of which <3% matched to viruses. The largest portion of these viral sequences was composed of bacterial phages. About 100–300 reads in each sample matched “multiple sclerosis-associated retrovirus”, but manual analysis showed that they were only repetitive sequences commonly present in mammalian genomes. All viral reads were also extracted for analysis, confirming that no BLV or its genetic variants or any other virus was detected in the nine tissue samples. NGS is not only useful for detecting microorganisms associated with infectious diseases, but also important for excluding an infective cause in scenarios where such a possibility is suspected. MDPI 2023-07-29 /pmc/articles/PMC10459180/ /pubmed/37623955 http://dx.doi.org/10.3390/pathogens12080995 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Brief Report Wernery, Ulrich Teng, Jade L. L. Ma, Yuanchao Kinne, Joerg Yeung, Man-Lung Anas, Safna Lau, Susanna K. P. Woo, Patrick C. Y. Usefulness of Next-Generation Sequencing in Excluding Bovine Leukemia Virus as a Cause of Adult Camel Leukosis in Dromedaries |
title | Usefulness of Next-Generation Sequencing in Excluding Bovine Leukemia Virus as a Cause of Adult Camel Leukosis in Dromedaries |
title_full | Usefulness of Next-Generation Sequencing in Excluding Bovine Leukemia Virus as a Cause of Adult Camel Leukosis in Dromedaries |
title_fullStr | Usefulness of Next-Generation Sequencing in Excluding Bovine Leukemia Virus as a Cause of Adult Camel Leukosis in Dromedaries |
title_full_unstemmed | Usefulness of Next-Generation Sequencing in Excluding Bovine Leukemia Virus as a Cause of Adult Camel Leukosis in Dromedaries |
title_short | Usefulness of Next-Generation Sequencing in Excluding Bovine Leukemia Virus as a Cause of Adult Camel Leukosis in Dromedaries |
title_sort | usefulness of next-generation sequencing in excluding bovine leukemia virus as a cause of adult camel leukosis in dromedaries |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10459180/ https://www.ncbi.nlm.nih.gov/pubmed/37623955 http://dx.doi.org/10.3390/pathogens12080995 |
work_keys_str_mv | AT werneryulrich usefulnessofnextgenerationsequencinginexcludingbovineleukemiavirusasacauseofadultcamelleukosisindromedaries AT tengjadell usefulnessofnextgenerationsequencinginexcludingbovineleukemiavirusasacauseofadultcamelleukosisindromedaries AT mayuanchao usefulnessofnextgenerationsequencinginexcludingbovineleukemiavirusasacauseofadultcamelleukosisindromedaries AT kinnejoerg usefulnessofnextgenerationsequencinginexcludingbovineleukemiavirusasacauseofadultcamelleukosisindromedaries AT yeungmanlung usefulnessofnextgenerationsequencinginexcludingbovineleukemiavirusasacauseofadultcamelleukosisindromedaries AT anassafna usefulnessofnextgenerationsequencinginexcludingbovineleukemiavirusasacauseofadultcamelleukosisindromedaries AT laususannakp usefulnessofnextgenerationsequencinginexcludingbovineleukemiavirusasacauseofadultcamelleukosisindromedaries AT woopatrickcy usefulnessofnextgenerationsequencinginexcludingbovineleukemiavirusasacauseofadultcamelleukosisindromedaries |