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Differences in the Susceptibility of Human Tubular Epithelial Cells for Infection with Orthohantaviruses

Diseases induced by infection with pathogenic orthohantaviruses are characterized by a pronounced organ-specific manifestation. Pathogenic Eurasian orthohantaviruses cause hemorrhagic fever with renal syndrome (HFRS) with often massive proteinuria. Therefore, the use of a relevant kidney cell cultur...

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Autores principales: Schreiber, Pamela, Friedrich, Ann-Kathrin, Gruber, Gefion, Nusshag, Christian, Boegelein, Lukas, Essbauer, Sandra, Uhrig, Josephine, Zeier, Martin, Krautkrämer, Ellen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10459294/
https://www.ncbi.nlm.nih.gov/pubmed/37632012
http://dx.doi.org/10.3390/v15081670
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author Schreiber, Pamela
Friedrich, Ann-Kathrin
Gruber, Gefion
Nusshag, Christian
Boegelein, Lukas
Essbauer, Sandra
Uhrig, Josephine
Zeier, Martin
Krautkrämer, Ellen
author_facet Schreiber, Pamela
Friedrich, Ann-Kathrin
Gruber, Gefion
Nusshag, Christian
Boegelein, Lukas
Essbauer, Sandra
Uhrig, Josephine
Zeier, Martin
Krautkrämer, Ellen
author_sort Schreiber, Pamela
collection PubMed
description Diseases induced by infection with pathogenic orthohantaviruses are characterized by a pronounced organ-specific manifestation. Pathogenic Eurasian orthohantaviruses cause hemorrhagic fever with renal syndrome (HFRS) with often massive proteinuria. Therefore, the use of a relevant kidney cell culture would be favorable to analyze the underlying cellular mechanisms of orthohantavirus-induced acute kidney injury (AKI). We tested different human tubular epithelial cell lines for their suitability as an in vitro infection model. Permissiveness and replication kinetics of highly pathogenic Hantaan virus (HTNV) and non-/low-pathogenic Tula virus (TULV) were analyzed in tubular epithelial cell lines and compared to human primary tubular epithelial cells. Ana-lysis of the cell line HK-2 revealed the same results for viral replication, morphological and functional effects as observed for HTNV in primary cells. In contrast, the cell lines RPTEC/TERT1 and TH1 demonstrated only poor infection rates after inoculation with HTNV and are unusable as an infection model. While pathogenic HNTV infects primary tubular and HK-2 cells, non-/low-pathogenic TULV infects neither primary tubular cells nor the cell line HK-2. Our results show that permissiveness of renal cells varies between orthohantaviruses with differences in pathogenicity and that HK-2 cells demonstrate a suitable in vitro model to study viral tropism and pathogenesis of orthohantavirus-induced AKI.
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spelling pubmed-104592942023-08-27 Differences in the Susceptibility of Human Tubular Epithelial Cells for Infection with Orthohantaviruses Schreiber, Pamela Friedrich, Ann-Kathrin Gruber, Gefion Nusshag, Christian Boegelein, Lukas Essbauer, Sandra Uhrig, Josephine Zeier, Martin Krautkrämer, Ellen Viruses Article Diseases induced by infection with pathogenic orthohantaviruses are characterized by a pronounced organ-specific manifestation. Pathogenic Eurasian orthohantaviruses cause hemorrhagic fever with renal syndrome (HFRS) with often massive proteinuria. Therefore, the use of a relevant kidney cell culture would be favorable to analyze the underlying cellular mechanisms of orthohantavirus-induced acute kidney injury (AKI). We tested different human tubular epithelial cell lines for their suitability as an in vitro infection model. Permissiveness and replication kinetics of highly pathogenic Hantaan virus (HTNV) and non-/low-pathogenic Tula virus (TULV) were analyzed in tubular epithelial cell lines and compared to human primary tubular epithelial cells. Ana-lysis of the cell line HK-2 revealed the same results for viral replication, morphological and functional effects as observed for HTNV in primary cells. In contrast, the cell lines RPTEC/TERT1 and TH1 demonstrated only poor infection rates after inoculation with HTNV and are unusable as an infection model. While pathogenic HNTV infects primary tubular and HK-2 cells, non-/low-pathogenic TULV infects neither primary tubular cells nor the cell line HK-2. Our results show that permissiveness of renal cells varies between orthohantaviruses with differences in pathogenicity and that HK-2 cells demonstrate a suitable in vitro model to study viral tropism and pathogenesis of orthohantavirus-induced AKI. MDPI 2023-07-31 /pmc/articles/PMC10459294/ /pubmed/37632012 http://dx.doi.org/10.3390/v15081670 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Schreiber, Pamela
Friedrich, Ann-Kathrin
Gruber, Gefion
Nusshag, Christian
Boegelein, Lukas
Essbauer, Sandra
Uhrig, Josephine
Zeier, Martin
Krautkrämer, Ellen
Differences in the Susceptibility of Human Tubular Epithelial Cells for Infection with Orthohantaviruses
title Differences in the Susceptibility of Human Tubular Epithelial Cells for Infection with Orthohantaviruses
title_full Differences in the Susceptibility of Human Tubular Epithelial Cells for Infection with Orthohantaviruses
title_fullStr Differences in the Susceptibility of Human Tubular Epithelial Cells for Infection with Orthohantaviruses
title_full_unstemmed Differences in the Susceptibility of Human Tubular Epithelial Cells for Infection with Orthohantaviruses
title_short Differences in the Susceptibility of Human Tubular Epithelial Cells for Infection with Orthohantaviruses
title_sort differences in the susceptibility of human tubular epithelial cells for infection with orthohantaviruses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10459294/
https://www.ncbi.nlm.nih.gov/pubmed/37632012
http://dx.doi.org/10.3390/v15081670
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