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Optimization of Callus Induction and Shoot Regeneration from Tomato Cotyledon Explants

Cultivated tomato (Solanum lycopersicum L.) is one of the most important horticultural crops in the world. The optimization of culture media for callus formation and tissue regeneration of different tomato genotypes presents numerous biotechnological applications. In this work, we have analyzed the...

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Autores principales: Yaroshko, Olha, Pasternak, Taras, Larriba, Eduardo, Pérez-Pérez, José Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10459365/
https://www.ncbi.nlm.nih.gov/pubmed/37631154
http://dx.doi.org/10.3390/plants12162942
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author Yaroshko, Olha
Pasternak, Taras
Larriba, Eduardo
Pérez-Pérez, José Manuel
author_facet Yaroshko, Olha
Pasternak, Taras
Larriba, Eduardo
Pérez-Pérez, José Manuel
author_sort Yaroshko, Olha
collection PubMed
description Cultivated tomato (Solanum lycopersicum L.) is one of the most important horticultural crops in the world. The optimization of culture media for callus formation and tissue regeneration of different tomato genotypes presents numerous biotechnological applications. In this work, we have analyzed the effect of different concentrations of zeatin and indole-3-acetic acid on the regeneration of cotyledon explants in tomato cultivars M82 and Micro-Tom. We evaluated regeneration parameters such as the percentage of callus formation and the area of callus formed, as well as the initiation percentage and the number of adventitious shoots. The best hormone combination produced shoot-like structures after 2–3 weeks. We observed the formation of leaf primordia from these structures after about 3–4 weeks. Upon transferring the regenerating micro-stems to a defined growth medium, it was possible to obtain whole plantlets between 4 and 6 weeks. This hormone combination was applied to other genotypes of S. lycopersicum, including commercial varieties and ancestral tomato varieties. Our method is suitable for obtaining many plantlets of different tomato genotypes from cotyledon explants in a very short time, with direct applications for plant transformation, use of gene editing techniques, and vegetative propagation of elite cultivars.
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spelling pubmed-104593652023-08-27 Optimization of Callus Induction and Shoot Regeneration from Tomato Cotyledon Explants Yaroshko, Olha Pasternak, Taras Larriba, Eduardo Pérez-Pérez, José Manuel Plants (Basel) Article Cultivated tomato (Solanum lycopersicum L.) is one of the most important horticultural crops in the world. The optimization of culture media for callus formation and tissue regeneration of different tomato genotypes presents numerous biotechnological applications. In this work, we have analyzed the effect of different concentrations of zeatin and indole-3-acetic acid on the regeneration of cotyledon explants in tomato cultivars M82 and Micro-Tom. We evaluated regeneration parameters such as the percentage of callus formation and the area of callus formed, as well as the initiation percentage and the number of adventitious shoots. The best hormone combination produced shoot-like structures after 2–3 weeks. We observed the formation of leaf primordia from these structures after about 3–4 weeks. Upon transferring the regenerating micro-stems to a defined growth medium, it was possible to obtain whole plantlets between 4 and 6 weeks. This hormone combination was applied to other genotypes of S. lycopersicum, including commercial varieties and ancestral tomato varieties. Our method is suitable for obtaining many plantlets of different tomato genotypes from cotyledon explants in a very short time, with direct applications for plant transformation, use of gene editing techniques, and vegetative propagation of elite cultivars. MDPI 2023-08-14 /pmc/articles/PMC10459365/ /pubmed/37631154 http://dx.doi.org/10.3390/plants12162942 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yaroshko, Olha
Pasternak, Taras
Larriba, Eduardo
Pérez-Pérez, José Manuel
Optimization of Callus Induction and Shoot Regeneration from Tomato Cotyledon Explants
title Optimization of Callus Induction and Shoot Regeneration from Tomato Cotyledon Explants
title_full Optimization of Callus Induction and Shoot Regeneration from Tomato Cotyledon Explants
title_fullStr Optimization of Callus Induction and Shoot Regeneration from Tomato Cotyledon Explants
title_full_unstemmed Optimization of Callus Induction and Shoot Regeneration from Tomato Cotyledon Explants
title_short Optimization of Callus Induction and Shoot Regeneration from Tomato Cotyledon Explants
title_sort optimization of callus induction and shoot regeneration from tomato cotyledon explants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10459365/
https://www.ncbi.nlm.nih.gov/pubmed/37631154
http://dx.doi.org/10.3390/plants12162942
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