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Corynebacterium ulcerans Infections in Eurasian Beavers (Castor fiber)

The Eurasian beaver (Castor fiber) has been reintroduced successfully in Germany since the 1990s. Since wildlife is an important source of zoonotic infectious diseases, monitoring of invasive and reintroduced species is crucial with respect to the One Health approach. Three Eurasian beavers were fou...

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Autores principales: Sting, Reinhard, Pölzelbauer, Catharina, Eisenberg, Tobias, Bonke, Rebecca, Blazey, Birgit, Peters, Martin, Riße, Karin, Sing, Andreas, Berger, Anja, Dangel, Alexandra, Rau, Jörg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10459376/
https://www.ncbi.nlm.nih.gov/pubmed/37623939
http://dx.doi.org/10.3390/pathogens12080979
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author Sting, Reinhard
Pölzelbauer, Catharina
Eisenberg, Tobias
Bonke, Rebecca
Blazey, Birgit
Peters, Martin
Riße, Karin
Sing, Andreas
Berger, Anja
Dangel, Alexandra
Rau, Jörg
author_facet Sting, Reinhard
Pölzelbauer, Catharina
Eisenberg, Tobias
Bonke, Rebecca
Blazey, Birgit
Peters, Martin
Riße, Karin
Sing, Andreas
Berger, Anja
Dangel, Alexandra
Rau, Jörg
author_sort Sting, Reinhard
collection PubMed
description The Eurasian beaver (Castor fiber) has been reintroduced successfully in Germany since the 1990s. Since wildlife is an important source of zoonotic infectious diseases, monitoring of invasive and reintroduced species is crucial with respect to the One Health approach. Three Eurasian beavers were found dead in the German federal states of Bavaria, North Rhine–Westphalia and Baden–Wuerttemberg in 2015, 2021 and 2022, respectively. During post-mortem examinations, Corynebacterium (C.) ulcerans could be isolated from the abscesses of two beavers and from the lungs of one of the animals. Identification of the bacterial isolates at the species level was carried out by spectroscopic analysis using MALDI-TOF MS, FT-IR and biochemical profiles and were verified by molecular analysis based on 16-23S internal transcribed spacer (ITS) region sequencing. Molecular characterization of the C. ulcerans isolates using whole-genome sequencing (WGS) revealed a genome size of about 2.5 Mbp and a GC content of 53.4%. Multilocus sequence typing (MLST) analysis classified all three isolates as the sequence type ST-332. A minimum spanning tree (MST) based on cgMLST allelic profiles, including 1211 core genes of the sequenced C. ulcerans isolates, showed that the beaver-derived isolates clearly group on the branch of C. ulcerans with the closest relationship to each other, in close similarity to an isolate from a dog. Antibiotic susceptibility testing revealed resistance to clindamycin and, in one strain, to erythromycin according to EUCAST, while all isolates were susceptible to the other antimicrobials tested.
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spelling pubmed-104593762023-08-27 Corynebacterium ulcerans Infections in Eurasian Beavers (Castor fiber) Sting, Reinhard Pölzelbauer, Catharina Eisenberg, Tobias Bonke, Rebecca Blazey, Birgit Peters, Martin Riße, Karin Sing, Andreas Berger, Anja Dangel, Alexandra Rau, Jörg Pathogens Article The Eurasian beaver (Castor fiber) has been reintroduced successfully in Germany since the 1990s. Since wildlife is an important source of zoonotic infectious diseases, monitoring of invasive and reintroduced species is crucial with respect to the One Health approach. Three Eurasian beavers were found dead in the German federal states of Bavaria, North Rhine–Westphalia and Baden–Wuerttemberg in 2015, 2021 and 2022, respectively. During post-mortem examinations, Corynebacterium (C.) ulcerans could be isolated from the abscesses of two beavers and from the lungs of one of the animals. Identification of the bacterial isolates at the species level was carried out by spectroscopic analysis using MALDI-TOF MS, FT-IR and biochemical profiles and were verified by molecular analysis based on 16-23S internal transcribed spacer (ITS) region sequencing. Molecular characterization of the C. ulcerans isolates using whole-genome sequencing (WGS) revealed a genome size of about 2.5 Mbp and a GC content of 53.4%. Multilocus sequence typing (MLST) analysis classified all three isolates as the sequence type ST-332. A minimum spanning tree (MST) based on cgMLST allelic profiles, including 1211 core genes of the sequenced C. ulcerans isolates, showed that the beaver-derived isolates clearly group on the branch of C. ulcerans with the closest relationship to each other, in close similarity to an isolate from a dog. Antibiotic susceptibility testing revealed resistance to clindamycin and, in one strain, to erythromycin according to EUCAST, while all isolates were susceptible to the other antimicrobials tested. MDPI 2023-07-26 /pmc/articles/PMC10459376/ /pubmed/37623939 http://dx.doi.org/10.3390/pathogens12080979 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sting, Reinhard
Pölzelbauer, Catharina
Eisenberg, Tobias
Bonke, Rebecca
Blazey, Birgit
Peters, Martin
Riße, Karin
Sing, Andreas
Berger, Anja
Dangel, Alexandra
Rau, Jörg
Corynebacterium ulcerans Infections in Eurasian Beavers (Castor fiber)
title Corynebacterium ulcerans Infections in Eurasian Beavers (Castor fiber)
title_full Corynebacterium ulcerans Infections in Eurasian Beavers (Castor fiber)
title_fullStr Corynebacterium ulcerans Infections in Eurasian Beavers (Castor fiber)
title_full_unstemmed Corynebacterium ulcerans Infections in Eurasian Beavers (Castor fiber)
title_short Corynebacterium ulcerans Infections in Eurasian Beavers (Castor fiber)
title_sort corynebacterium ulcerans infections in eurasian beavers (castor fiber)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10459376/
https://www.ncbi.nlm.nih.gov/pubmed/37623939
http://dx.doi.org/10.3390/pathogens12080979
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