Cargando…

The Efficient Extraction Method of Collagen from Deteriorated Leather Artifacts

Collagen is the most crucial component of leather artifacts and analyzing collagen can provide vital information for studying and conserving such artifacts. However, collagen in leather artifacts often faces challenges such as degradation, denaturation, and contamination, which make it difficult to...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Li, Zhang, Meng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10459694/
https://www.ncbi.nlm.nih.gov/pubmed/37631517
http://dx.doi.org/10.3390/polym15163459
_version_ 1785097473380843520
author Li, Li
Zhang, Meng
author_facet Li, Li
Zhang, Meng
author_sort Li, Li
collection PubMed
description Collagen is the most crucial component of leather artifacts and analyzing collagen can provide vital information for studying and conserving such artifacts. However, collagen in leather artifacts often faces challenges such as degradation, denaturation, and contamination, which make it difficult to achieve an ideal protein extract using traditional extraction methods. This study aimed to find an efficient collagen extraction strategy for aging leather by comparing and improving commonly used methods. The results of comparing different extraction methods indicated that a NaOH solution was highly effective in extracting collagen from aged leather. To determine the optimal conditions for collagen extraction from the NaOH solution, we conducted orthogonal experiments. The results revealed that a NaOH concentration of 0.05 mol/L, a dissolution temperature of 80 °C, and a dissolution time of 12 h were the most favorable conditions. To validate the effectiveness of this method, we performed SDS-PAGE and biological mass spectrometry tests on collagen extracts from leather samples with varying degrees of aging. All collagen extracts exhibited distinct bands in the gel, and the molecular weight of collagen in each sample exceeded 20 kDa. Furthermore, even with a reduced sample mass of 1 mg (micro-destructive sampling), biological mass spectrometry identified 124 peptides in the protein extract. Notably, four of these peptides were unique to cattle hide collagen and were not present in the collagen of pig, sheep, horse, deer, or human skins. These experimental findings confirm the efficacy of the NaOH solution for extracting collagen from aging leather, suggesting that it can serve as a significant method for collagen identification and analysis in leather artifacts.
format Online
Article
Text
id pubmed-10459694
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-104596942023-08-27 The Efficient Extraction Method of Collagen from Deteriorated Leather Artifacts Li, Li Zhang, Meng Polymers (Basel) Article Collagen is the most crucial component of leather artifacts and analyzing collagen can provide vital information for studying and conserving such artifacts. However, collagen in leather artifacts often faces challenges such as degradation, denaturation, and contamination, which make it difficult to achieve an ideal protein extract using traditional extraction methods. This study aimed to find an efficient collagen extraction strategy for aging leather by comparing and improving commonly used methods. The results of comparing different extraction methods indicated that a NaOH solution was highly effective in extracting collagen from aged leather. To determine the optimal conditions for collagen extraction from the NaOH solution, we conducted orthogonal experiments. The results revealed that a NaOH concentration of 0.05 mol/L, a dissolution temperature of 80 °C, and a dissolution time of 12 h were the most favorable conditions. To validate the effectiveness of this method, we performed SDS-PAGE and biological mass spectrometry tests on collagen extracts from leather samples with varying degrees of aging. All collagen extracts exhibited distinct bands in the gel, and the molecular weight of collagen in each sample exceeded 20 kDa. Furthermore, even with a reduced sample mass of 1 mg (micro-destructive sampling), biological mass spectrometry identified 124 peptides in the protein extract. Notably, four of these peptides were unique to cattle hide collagen and were not present in the collagen of pig, sheep, horse, deer, or human skins. These experimental findings confirm the efficacy of the NaOH solution for extracting collagen from aging leather, suggesting that it can serve as a significant method for collagen identification and analysis in leather artifacts. MDPI 2023-08-18 /pmc/articles/PMC10459694/ /pubmed/37631517 http://dx.doi.org/10.3390/polym15163459 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Li
Zhang, Meng
The Efficient Extraction Method of Collagen from Deteriorated Leather Artifacts
title The Efficient Extraction Method of Collagen from Deteriorated Leather Artifacts
title_full The Efficient Extraction Method of Collagen from Deteriorated Leather Artifacts
title_fullStr The Efficient Extraction Method of Collagen from Deteriorated Leather Artifacts
title_full_unstemmed The Efficient Extraction Method of Collagen from Deteriorated Leather Artifacts
title_short The Efficient Extraction Method of Collagen from Deteriorated Leather Artifacts
title_sort efficient extraction method of collagen from deteriorated leather artifacts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10459694/
https://www.ncbi.nlm.nih.gov/pubmed/37631517
http://dx.doi.org/10.3390/polym15163459
work_keys_str_mv AT lili theefficientextractionmethodofcollagenfromdeterioratedleatherartifacts
AT zhangmeng theefficientextractionmethodofcollagenfromdeterioratedleatherartifacts
AT lili efficientextractionmethodofcollagenfromdeterioratedleatherartifacts
AT zhangmeng efficientextractionmethodofcollagenfromdeterioratedleatherartifacts