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Analysis of the Effect of the TRPC4/TRPC5 Blocker, ML204, in Sucrose-Induced Metabolic Imbalance
Sugar-induced metabolic imbalances are a major health problem since an excessive consumption of saccharides has been linked to greater obesity rates at a global level. Sucrose, a disaccharide composed of 50% glucose and 50% fructose, is commonly used in the food industry and found in a range of fast...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10459798/ https://www.ncbi.nlm.nih.gov/pubmed/37631015 http://dx.doi.org/10.3390/ph16081100 |
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author | Araújo, Mizael C. Soczek, Suzany H. S. Pontes, Jaqueline P. Pinto, Bruno A. S. França, Lucas M. Soley, Bruna da Silva Santos, Gabriela S. Saminez, Warlison F. de Silva Fernandes, Fernanda K. M. Lima, João L. do Carmo Maria-Ferreira, Daniele Rodrigues, João F. S. Quintão, Nara L. M. Monteiro-Neto, Valério Paes, Antônio M. A. Fernandes, Elizabeth S. |
author_facet | Araújo, Mizael C. Soczek, Suzany H. S. Pontes, Jaqueline P. Pinto, Bruno A. S. França, Lucas M. Soley, Bruna da Silva Santos, Gabriela S. Saminez, Warlison F. de Silva Fernandes, Fernanda K. M. Lima, João L. do Carmo Maria-Ferreira, Daniele Rodrigues, João F. S. Quintão, Nara L. M. Monteiro-Neto, Valério Paes, Antônio M. A. Fernandes, Elizabeth S. |
author_sort | Araújo, Mizael C. |
collection | PubMed |
description | Sugar-induced metabolic imbalances are a major health problem since an excessive consumption of saccharides has been linked to greater obesity rates at a global level. Sucrose, a disaccharide composed of 50% glucose and 50% fructose, is commonly used in the food industry and found in a range of fast, restaurant, and processed foods. Herein, we investigated the effects of a TRPC4/TRPC5 blocker, ML204, in the metabolic imbalances triggered by early exposure to sucrose-enriched diet in mice. TRPC4 and TRPC5 belong to the family of non-selective Ca(+2) channels known as transient receptor potential channels. High-sucrose (HS)-fed animals with hyperglycaemia and dyslipidaemia, were accompanied by increased body mass index. mesenteric adipose tissue accumulation with larger diameter cells and hepatic steatosis in comparison to those fed normal diet. HS mice also exhibited enhanced adipose, liver, and pancreas TNFα and VEGF levels. ML204 exacerbated hyperglycaemia, dyslipidaemia, fat tissue deposition, hepatic steatosis, and adipose tissue and liver TNFα in HS-fed mice. Normal mice treated with the blocker had greater hepatic steatosis and adipose tissue cell numbers/diameter than those receiving vehicle, but showed no significant changes in tissue inflammation, glucose, and lipid levels. The results indicate that TRPC4/TRPC5 protect against the metabolic imbalances caused by HS ingestion. |
format | Online Article Text |
id | pubmed-10459798 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104597982023-08-27 Analysis of the Effect of the TRPC4/TRPC5 Blocker, ML204, in Sucrose-Induced Metabolic Imbalance Araújo, Mizael C. Soczek, Suzany H. S. Pontes, Jaqueline P. Pinto, Bruno A. S. França, Lucas M. Soley, Bruna da Silva Santos, Gabriela S. Saminez, Warlison F. de Silva Fernandes, Fernanda K. M. Lima, João L. do Carmo Maria-Ferreira, Daniele Rodrigues, João F. S. Quintão, Nara L. M. Monteiro-Neto, Valério Paes, Antônio M. A. Fernandes, Elizabeth S. Pharmaceuticals (Basel) Article Sugar-induced metabolic imbalances are a major health problem since an excessive consumption of saccharides has been linked to greater obesity rates at a global level. Sucrose, a disaccharide composed of 50% glucose and 50% fructose, is commonly used in the food industry and found in a range of fast, restaurant, and processed foods. Herein, we investigated the effects of a TRPC4/TRPC5 blocker, ML204, in the metabolic imbalances triggered by early exposure to sucrose-enriched diet in mice. TRPC4 and TRPC5 belong to the family of non-selective Ca(+2) channels known as transient receptor potential channels. High-sucrose (HS)-fed animals with hyperglycaemia and dyslipidaemia, were accompanied by increased body mass index. mesenteric adipose tissue accumulation with larger diameter cells and hepatic steatosis in comparison to those fed normal diet. HS mice also exhibited enhanced adipose, liver, and pancreas TNFα and VEGF levels. ML204 exacerbated hyperglycaemia, dyslipidaemia, fat tissue deposition, hepatic steatosis, and adipose tissue and liver TNFα in HS-fed mice. Normal mice treated with the blocker had greater hepatic steatosis and adipose tissue cell numbers/diameter than those receiving vehicle, but showed no significant changes in tissue inflammation, glucose, and lipid levels. The results indicate that TRPC4/TRPC5 protect against the metabolic imbalances caused by HS ingestion. MDPI 2023-08-03 /pmc/articles/PMC10459798/ /pubmed/37631015 http://dx.doi.org/10.3390/ph16081100 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Araújo, Mizael C. Soczek, Suzany H. S. Pontes, Jaqueline P. Pinto, Bruno A. S. França, Lucas M. Soley, Bruna da Silva Santos, Gabriela S. Saminez, Warlison F. de Silva Fernandes, Fernanda K. M. Lima, João L. do Carmo Maria-Ferreira, Daniele Rodrigues, João F. S. Quintão, Nara L. M. Monteiro-Neto, Valério Paes, Antônio M. A. Fernandes, Elizabeth S. Analysis of the Effect of the TRPC4/TRPC5 Blocker, ML204, in Sucrose-Induced Metabolic Imbalance |
title | Analysis of the Effect of the TRPC4/TRPC5 Blocker, ML204, in Sucrose-Induced Metabolic Imbalance |
title_full | Analysis of the Effect of the TRPC4/TRPC5 Blocker, ML204, in Sucrose-Induced Metabolic Imbalance |
title_fullStr | Analysis of the Effect of the TRPC4/TRPC5 Blocker, ML204, in Sucrose-Induced Metabolic Imbalance |
title_full_unstemmed | Analysis of the Effect of the TRPC4/TRPC5 Blocker, ML204, in Sucrose-Induced Metabolic Imbalance |
title_short | Analysis of the Effect of the TRPC4/TRPC5 Blocker, ML204, in Sucrose-Induced Metabolic Imbalance |
title_sort | analysis of the effect of the trpc4/trpc5 blocker, ml204, in sucrose-induced metabolic imbalance |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10459798/ https://www.ncbi.nlm.nih.gov/pubmed/37631015 http://dx.doi.org/10.3390/ph16081100 |
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