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Influence of Different Diets on the Degradation of Sulfasalazine by Colon Bacteria Determined Using MimiCol(3)

The microbiome of the colon is characterized by its great diversity. This varies not only intra- but also interindividually and is influenced by endogenous and exogenous factors, such as dietary and lifestyle factors. The aim of this work was to investigate the extent to which the degradation of the...

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Autores principales: Seradj, Dariah-Sohreh, Beeck, Regine, Haase, Annika, Krause, Julius, Schick, Philipp, Weitschies, Werner
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10459966/
https://www.ncbi.nlm.nih.gov/pubmed/37631043
http://dx.doi.org/10.3390/ph16081128
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author Seradj, Dariah-Sohreh
Beeck, Regine
Haase, Annika
Krause, Julius
Schick, Philipp
Weitschies, Werner
author_facet Seradj, Dariah-Sohreh
Beeck, Regine
Haase, Annika
Krause, Julius
Schick, Philipp
Weitschies, Werner
author_sort Seradj, Dariah-Sohreh
collection PubMed
description The microbiome of the colon is characterized by its great diversity. This varies not only intra- but also interindividually and is influenced by endogenous and exogenous factors, such as dietary and lifestyle factors. The aim of this work was to investigate the extent to which the degradation of the drug sulfasalazine is influenced by different microbiota. Therefore, the in vitro model MimiCol(3) was used, which represents the physiological conditions of the ascending colon. In addition to a representative physiological volume, the pH value, redox potential and an anaerobic atmosphere are important to provide the bacteria with the best possible growth conditions. Stool samples were taken from three healthy subjects, comparing omnivorous, vegetarian and meat-rich diets, and cultured for 24 h. However, the nutrient medium used for cultivation led to the alignment of the bacterial composition of the microbiota. The previously observed differences between the diets could not be maintained. Nevertheless, the similar degradation of sulfasalazine was observed in all microbiota studied in MimiCol(3). This makes MimiCol(3) a suitable in vitro model for metabolism studies in the gut microbiome.
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spelling pubmed-104599662023-08-27 Influence of Different Diets on the Degradation of Sulfasalazine by Colon Bacteria Determined Using MimiCol(3) Seradj, Dariah-Sohreh Beeck, Regine Haase, Annika Krause, Julius Schick, Philipp Weitschies, Werner Pharmaceuticals (Basel) Article The microbiome of the colon is characterized by its great diversity. This varies not only intra- but also interindividually and is influenced by endogenous and exogenous factors, such as dietary and lifestyle factors. The aim of this work was to investigate the extent to which the degradation of the drug sulfasalazine is influenced by different microbiota. Therefore, the in vitro model MimiCol(3) was used, which represents the physiological conditions of the ascending colon. In addition to a representative physiological volume, the pH value, redox potential and an anaerobic atmosphere are important to provide the bacteria with the best possible growth conditions. Stool samples were taken from three healthy subjects, comparing omnivorous, vegetarian and meat-rich diets, and cultured for 24 h. However, the nutrient medium used for cultivation led to the alignment of the bacterial composition of the microbiota. The previously observed differences between the diets could not be maintained. Nevertheless, the similar degradation of sulfasalazine was observed in all microbiota studied in MimiCol(3). This makes MimiCol(3) a suitable in vitro model for metabolism studies in the gut microbiome. MDPI 2023-08-10 /pmc/articles/PMC10459966/ /pubmed/37631043 http://dx.doi.org/10.3390/ph16081128 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Seradj, Dariah-Sohreh
Beeck, Regine
Haase, Annika
Krause, Julius
Schick, Philipp
Weitschies, Werner
Influence of Different Diets on the Degradation of Sulfasalazine by Colon Bacteria Determined Using MimiCol(3)
title Influence of Different Diets on the Degradation of Sulfasalazine by Colon Bacteria Determined Using MimiCol(3)
title_full Influence of Different Diets on the Degradation of Sulfasalazine by Colon Bacteria Determined Using MimiCol(3)
title_fullStr Influence of Different Diets on the Degradation of Sulfasalazine by Colon Bacteria Determined Using MimiCol(3)
title_full_unstemmed Influence of Different Diets on the Degradation of Sulfasalazine by Colon Bacteria Determined Using MimiCol(3)
title_short Influence of Different Diets on the Degradation of Sulfasalazine by Colon Bacteria Determined Using MimiCol(3)
title_sort influence of different diets on the degradation of sulfasalazine by colon bacteria determined using mimicol(3)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10459966/
https://www.ncbi.nlm.nih.gov/pubmed/37631043
http://dx.doi.org/10.3390/ph16081128
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