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Bioinformatic Surveillance Leads to Discovery of Two Novel Putative Bunyaviruses Associated with Black Soldier Fly

The black soldier fly (Hermetia illucens, BSF) has emerged as an industrial insect of high promise because of its ability to convert organic waste into nutritious feedstock, making it an environmentally sustainable alternative protein source. As global interest rises, rearing efforts have also been...

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Autores principales: Walt, Hunter K., Kooienga, Emilia, Cammack, Jonathan A., Tomberlin, Jeffery K., Jordan, Heather R., Meyer, Florencia, Hoffmann, Federico G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10460066/
https://www.ncbi.nlm.nih.gov/pubmed/37631997
http://dx.doi.org/10.3390/v15081654
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author Walt, Hunter K.
Kooienga, Emilia
Cammack, Jonathan A.
Tomberlin, Jeffery K.
Jordan, Heather R.
Meyer, Florencia
Hoffmann, Federico G.
author_facet Walt, Hunter K.
Kooienga, Emilia
Cammack, Jonathan A.
Tomberlin, Jeffery K.
Jordan, Heather R.
Meyer, Florencia
Hoffmann, Federico G.
author_sort Walt, Hunter K.
collection PubMed
description The black soldier fly (Hermetia illucens, BSF) has emerged as an industrial insect of high promise because of its ability to convert organic waste into nutritious feedstock, making it an environmentally sustainable alternative protein source. As global interest rises, rearing efforts have also been upscaled, which is highly conducive to pathogen transmission. Viral epidemics have stifled mass-rearing efforts of other insects of economic importance, such as crickets, silkworms, and honeybees, but little is known about the viruses that associate with BSF. Although BSFs are thought to be unusually resistant to pathogens because of their expansive antimicrobial gene repertoire, surveillance techniques could be useful in identifying emerging pathogens and common BSF microbes. In this study, we used high-throughput sequencing data to survey BSF larvae and frass samples, and we identified two novel bunyavirus-like sequences. Our phylogenetic analysis grouped one in the family Nairoviridae and the other with two unclassified bunyaviruses. We describe these putative novel viruses as BSF Nairovirus-like 1 and BSF uncharacterized bunyavirus-like 1. We identified candidate segments for the full BSF Nairovirus-like 1 genome using a technique based on transcript co-occurrence and only a partial genome for BSF uncharacterized bunyavirus-like 1. These results emphasize the value of routine BSF colony surveillance and add to the number of viruses associated with BSF.
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spelling pubmed-104600662023-08-27 Bioinformatic Surveillance Leads to Discovery of Two Novel Putative Bunyaviruses Associated with Black Soldier Fly Walt, Hunter K. Kooienga, Emilia Cammack, Jonathan A. Tomberlin, Jeffery K. Jordan, Heather R. Meyer, Florencia Hoffmann, Federico G. Viruses Communication The black soldier fly (Hermetia illucens, BSF) has emerged as an industrial insect of high promise because of its ability to convert organic waste into nutritious feedstock, making it an environmentally sustainable alternative protein source. As global interest rises, rearing efforts have also been upscaled, which is highly conducive to pathogen transmission. Viral epidemics have stifled mass-rearing efforts of other insects of economic importance, such as crickets, silkworms, and honeybees, but little is known about the viruses that associate with BSF. Although BSFs are thought to be unusually resistant to pathogens because of their expansive antimicrobial gene repertoire, surveillance techniques could be useful in identifying emerging pathogens and common BSF microbes. In this study, we used high-throughput sequencing data to survey BSF larvae and frass samples, and we identified two novel bunyavirus-like sequences. Our phylogenetic analysis grouped one in the family Nairoviridae and the other with two unclassified bunyaviruses. We describe these putative novel viruses as BSF Nairovirus-like 1 and BSF uncharacterized bunyavirus-like 1. We identified candidate segments for the full BSF Nairovirus-like 1 genome using a technique based on transcript co-occurrence and only a partial genome for BSF uncharacterized bunyavirus-like 1. These results emphasize the value of routine BSF colony surveillance and add to the number of viruses associated with BSF. MDPI 2023-07-29 /pmc/articles/PMC10460066/ /pubmed/37631997 http://dx.doi.org/10.3390/v15081654 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Walt, Hunter K.
Kooienga, Emilia
Cammack, Jonathan A.
Tomberlin, Jeffery K.
Jordan, Heather R.
Meyer, Florencia
Hoffmann, Federico G.
Bioinformatic Surveillance Leads to Discovery of Two Novel Putative Bunyaviruses Associated with Black Soldier Fly
title Bioinformatic Surveillance Leads to Discovery of Two Novel Putative Bunyaviruses Associated with Black Soldier Fly
title_full Bioinformatic Surveillance Leads to Discovery of Two Novel Putative Bunyaviruses Associated with Black Soldier Fly
title_fullStr Bioinformatic Surveillance Leads to Discovery of Two Novel Putative Bunyaviruses Associated with Black Soldier Fly
title_full_unstemmed Bioinformatic Surveillance Leads to Discovery of Two Novel Putative Bunyaviruses Associated with Black Soldier Fly
title_short Bioinformatic Surveillance Leads to Discovery of Two Novel Putative Bunyaviruses Associated with Black Soldier Fly
title_sort bioinformatic surveillance leads to discovery of two novel putative bunyaviruses associated with black soldier fly
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10460066/
https://www.ncbi.nlm.nih.gov/pubmed/37631997
http://dx.doi.org/10.3390/v15081654
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