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Biodegradation of Uric Acid by Bacillus paramycoides-YC02

High serum uric acid levels, known as hyperuricemia (HUA), are associated with an increased risk of developing gout, chronic kidney disease, cardiovascular disease, diabetes, and other metabolic syndromes. In this study, a promising bacterial strain capable of biodegrading uric acid (UA) was success...

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Autores principales: Cao, Xiaoyu, Cai, Jingyuan, Zhang, Yu, Liu, Chao, Song, Meijie, Xu, Qianqian, Liu, Yang, Yan, Hai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10460076/
https://www.ncbi.nlm.nih.gov/pubmed/37630550
http://dx.doi.org/10.3390/microorganisms11081989
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author Cao, Xiaoyu
Cai, Jingyuan
Zhang, Yu
Liu, Chao
Song, Meijie
Xu, Qianqian
Liu, Yang
Yan, Hai
author_facet Cao, Xiaoyu
Cai, Jingyuan
Zhang, Yu
Liu, Chao
Song, Meijie
Xu, Qianqian
Liu, Yang
Yan, Hai
author_sort Cao, Xiaoyu
collection PubMed
description High serum uric acid levels, known as hyperuricemia (HUA), are associated with an increased risk of developing gout, chronic kidney disease, cardiovascular disease, diabetes, and other metabolic syndromes. In this study, a promising bacterial strain capable of biodegrading uric acid (UA) was successfully isolated from Baijiu cellar mud using UA as the sole carbon and energy source. The bacterial strain was identified as Bacillus paramycoides-YC02 through 16S rDNA sequence analysis. Under optimal culture conditions at an initial pH of 7.0 and 38 °C, YC02 completely biodegraded an initial UA concentration of 500 mg/L within 48 h. Furthermore, cell-free extracts of YC02 were found to catalyze and remove UA. These results demonstrate the strong biodegradation ability of YC02 toward UA. To gain further insight into the mechanisms underlying UA biodegradation by YC02, the draft genome of YC02 was sequenced using Illumina HiSeq. Subsequent analysis revealed the presence of gene1779 and gene2008, which encode for riboflavin kinase, flavin mononucleotide adenylyl transferase, and flavin adenine dinucleotide (FAD)-dependent urate hydroxylase. This annotation was based on GO or the KEEG database. These enzymes play a crucial role in the metabolism pathway, converting vitamin B(2) to FAD and subsequently converting UA to 5-hydroxyisourate (HIU) with the assistance of FAD. Notably, HIU undergoes a slow non-enzymatic breakdown into 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU) and (S)-allantoin. The findings of this study provide valuable insights into the metabolism pathway of UA biodegradation by B. paramycoides-YC02 and offer a potential avenue for the development of bacterioactive drugs against HUA and gout.
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spelling pubmed-104600762023-08-27 Biodegradation of Uric Acid by Bacillus paramycoides-YC02 Cao, Xiaoyu Cai, Jingyuan Zhang, Yu Liu, Chao Song, Meijie Xu, Qianqian Liu, Yang Yan, Hai Microorganisms Article High serum uric acid levels, known as hyperuricemia (HUA), are associated with an increased risk of developing gout, chronic kidney disease, cardiovascular disease, diabetes, and other metabolic syndromes. In this study, a promising bacterial strain capable of biodegrading uric acid (UA) was successfully isolated from Baijiu cellar mud using UA as the sole carbon and energy source. The bacterial strain was identified as Bacillus paramycoides-YC02 through 16S rDNA sequence analysis. Under optimal culture conditions at an initial pH of 7.0 and 38 °C, YC02 completely biodegraded an initial UA concentration of 500 mg/L within 48 h. Furthermore, cell-free extracts of YC02 were found to catalyze and remove UA. These results demonstrate the strong biodegradation ability of YC02 toward UA. To gain further insight into the mechanisms underlying UA biodegradation by YC02, the draft genome of YC02 was sequenced using Illumina HiSeq. Subsequent analysis revealed the presence of gene1779 and gene2008, which encode for riboflavin kinase, flavin mononucleotide adenylyl transferase, and flavin adenine dinucleotide (FAD)-dependent urate hydroxylase. This annotation was based on GO or the KEEG database. These enzymes play a crucial role in the metabolism pathway, converting vitamin B(2) to FAD and subsequently converting UA to 5-hydroxyisourate (HIU) with the assistance of FAD. Notably, HIU undergoes a slow non-enzymatic breakdown into 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU) and (S)-allantoin. The findings of this study provide valuable insights into the metabolism pathway of UA biodegradation by B. paramycoides-YC02 and offer a potential avenue for the development of bacterioactive drugs against HUA and gout. MDPI 2023-08-02 /pmc/articles/PMC10460076/ /pubmed/37630550 http://dx.doi.org/10.3390/microorganisms11081989 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cao, Xiaoyu
Cai, Jingyuan
Zhang, Yu
Liu, Chao
Song, Meijie
Xu, Qianqian
Liu, Yang
Yan, Hai
Biodegradation of Uric Acid by Bacillus paramycoides-YC02
title Biodegradation of Uric Acid by Bacillus paramycoides-YC02
title_full Biodegradation of Uric Acid by Bacillus paramycoides-YC02
title_fullStr Biodegradation of Uric Acid by Bacillus paramycoides-YC02
title_full_unstemmed Biodegradation of Uric Acid by Bacillus paramycoides-YC02
title_short Biodegradation of Uric Acid by Bacillus paramycoides-YC02
title_sort biodegradation of uric acid by bacillus paramycoides-yc02
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10460076/
https://www.ncbi.nlm.nih.gov/pubmed/37630550
http://dx.doi.org/10.3390/microorganisms11081989
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