Cargando…
Biodegradation of Uric Acid by Bacillus paramycoides-YC02
High serum uric acid levels, known as hyperuricemia (HUA), are associated with an increased risk of developing gout, chronic kidney disease, cardiovascular disease, diabetes, and other metabolic syndromes. In this study, a promising bacterial strain capable of biodegrading uric acid (UA) was success...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10460076/ https://www.ncbi.nlm.nih.gov/pubmed/37630550 http://dx.doi.org/10.3390/microorganisms11081989 |
_version_ | 1785097565073571840 |
---|---|
author | Cao, Xiaoyu Cai, Jingyuan Zhang, Yu Liu, Chao Song, Meijie Xu, Qianqian Liu, Yang Yan, Hai |
author_facet | Cao, Xiaoyu Cai, Jingyuan Zhang, Yu Liu, Chao Song, Meijie Xu, Qianqian Liu, Yang Yan, Hai |
author_sort | Cao, Xiaoyu |
collection | PubMed |
description | High serum uric acid levels, known as hyperuricemia (HUA), are associated with an increased risk of developing gout, chronic kidney disease, cardiovascular disease, diabetes, and other metabolic syndromes. In this study, a promising bacterial strain capable of biodegrading uric acid (UA) was successfully isolated from Baijiu cellar mud using UA as the sole carbon and energy source. The bacterial strain was identified as Bacillus paramycoides-YC02 through 16S rDNA sequence analysis. Under optimal culture conditions at an initial pH of 7.0 and 38 °C, YC02 completely biodegraded an initial UA concentration of 500 mg/L within 48 h. Furthermore, cell-free extracts of YC02 were found to catalyze and remove UA. These results demonstrate the strong biodegradation ability of YC02 toward UA. To gain further insight into the mechanisms underlying UA biodegradation by YC02, the draft genome of YC02 was sequenced using Illumina HiSeq. Subsequent analysis revealed the presence of gene1779 and gene2008, which encode for riboflavin kinase, flavin mononucleotide adenylyl transferase, and flavin adenine dinucleotide (FAD)-dependent urate hydroxylase. This annotation was based on GO or the KEEG database. These enzymes play a crucial role in the metabolism pathway, converting vitamin B(2) to FAD and subsequently converting UA to 5-hydroxyisourate (HIU) with the assistance of FAD. Notably, HIU undergoes a slow non-enzymatic breakdown into 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU) and (S)-allantoin. The findings of this study provide valuable insights into the metabolism pathway of UA biodegradation by B. paramycoides-YC02 and offer a potential avenue for the development of bacterioactive drugs against HUA and gout. |
format | Online Article Text |
id | pubmed-10460076 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104600762023-08-27 Biodegradation of Uric Acid by Bacillus paramycoides-YC02 Cao, Xiaoyu Cai, Jingyuan Zhang, Yu Liu, Chao Song, Meijie Xu, Qianqian Liu, Yang Yan, Hai Microorganisms Article High serum uric acid levels, known as hyperuricemia (HUA), are associated with an increased risk of developing gout, chronic kidney disease, cardiovascular disease, diabetes, and other metabolic syndromes. In this study, a promising bacterial strain capable of biodegrading uric acid (UA) was successfully isolated from Baijiu cellar mud using UA as the sole carbon and energy source. The bacterial strain was identified as Bacillus paramycoides-YC02 through 16S rDNA sequence analysis. Under optimal culture conditions at an initial pH of 7.0 and 38 °C, YC02 completely biodegraded an initial UA concentration of 500 mg/L within 48 h. Furthermore, cell-free extracts of YC02 were found to catalyze and remove UA. These results demonstrate the strong biodegradation ability of YC02 toward UA. To gain further insight into the mechanisms underlying UA biodegradation by YC02, the draft genome of YC02 was sequenced using Illumina HiSeq. Subsequent analysis revealed the presence of gene1779 and gene2008, which encode for riboflavin kinase, flavin mononucleotide adenylyl transferase, and flavin adenine dinucleotide (FAD)-dependent urate hydroxylase. This annotation was based on GO or the KEEG database. These enzymes play a crucial role in the metabolism pathway, converting vitamin B(2) to FAD and subsequently converting UA to 5-hydroxyisourate (HIU) with the assistance of FAD. Notably, HIU undergoes a slow non-enzymatic breakdown into 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU) and (S)-allantoin. The findings of this study provide valuable insights into the metabolism pathway of UA biodegradation by B. paramycoides-YC02 and offer a potential avenue for the development of bacterioactive drugs against HUA and gout. MDPI 2023-08-02 /pmc/articles/PMC10460076/ /pubmed/37630550 http://dx.doi.org/10.3390/microorganisms11081989 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cao, Xiaoyu Cai, Jingyuan Zhang, Yu Liu, Chao Song, Meijie Xu, Qianqian Liu, Yang Yan, Hai Biodegradation of Uric Acid by Bacillus paramycoides-YC02 |
title | Biodegradation of Uric Acid by Bacillus paramycoides-YC02 |
title_full | Biodegradation of Uric Acid by Bacillus paramycoides-YC02 |
title_fullStr | Biodegradation of Uric Acid by Bacillus paramycoides-YC02 |
title_full_unstemmed | Biodegradation of Uric Acid by Bacillus paramycoides-YC02 |
title_short | Biodegradation of Uric Acid by Bacillus paramycoides-YC02 |
title_sort | biodegradation of uric acid by bacillus paramycoides-yc02 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10460076/ https://www.ncbi.nlm.nih.gov/pubmed/37630550 http://dx.doi.org/10.3390/microorganisms11081989 |
work_keys_str_mv | AT caoxiaoyu biodegradationofuricacidbybacillusparamycoidesyc02 AT caijingyuan biodegradationofuricacidbybacillusparamycoidesyc02 AT zhangyu biodegradationofuricacidbybacillusparamycoidesyc02 AT liuchao biodegradationofuricacidbybacillusparamycoidesyc02 AT songmeijie biodegradationofuricacidbybacillusparamycoidesyc02 AT xuqianqian biodegradationofuricacidbybacillusparamycoidesyc02 AT liuyang biodegradationofuricacidbybacillusparamycoidesyc02 AT yanhai biodegradationofuricacidbybacillusparamycoidesyc02 |