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Dyslipidemia initiates keratinocytes proliferation through upregulation of lncRNA NEAT in psoriasis patients
BACKGROUND: Psoriasis is a chronic inflammatory immune-mediated and hyper proliferative skin disorder that has underlying genetic factors. Psoriasis can result from interaction of cytokines between keratinocytes and T-lymphocytes. NEAT is a lncRNA involved in immune modulation and has been previousl...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10460715/ https://www.ncbi.nlm.nih.gov/pubmed/37531036 http://dx.doi.org/10.1007/s11033-023-08527-w |
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author | Mostafa, Abeer Sabry, Dina Aboraia, Nesreen Fawzy, Ahmed Abou-Elalla, Amany A. |
author_facet | Mostafa, Abeer Sabry, Dina Aboraia, Nesreen Fawzy, Ahmed Abou-Elalla, Amany A. |
author_sort | Mostafa, Abeer |
collection | PubMed |
description | BACKGROUND: Psoriasis is a chronic inflammatory immune-mediated and hyper proliferative skin disorder that has underlying genetic factors. Psoriasis can result from interaction of cytokines between keratinocytes and T-lymphocytes. NEAT is a lncRNA involved in immune modulation and has been previously studied in cancers. This study aims to clarify the unprecedented role of NEAT in psoriasis pathogenesis. METHODS: The study was conducted on 50 healthy control subjects and 50 psoriasis patients. Blood samples from all participants were collected for analysis of their lipid profile. qRT-PCR was done for lncRNA NEAT, TNF-α, VEGF genes expression. The levels of ROS and caspase-3 were estimated by ELISA. ROC analysis was done to detect the diagnostic value of lncRNA NEAT gene expression. RESULTS: Dyslipidemia is more prevalent among psoriasis patients. A significant up regulation in lncRNA NEAT, TNF-α, VEGF genes expression (p value˂0.001) in psoriasis patients in addition to significant increase in ROS and caspase-3 levels (p value˂0.001) in compare to controls. Additionally, a positive significant correlation between TNF-α, ROS, NEAT, caspase-3 and dyslipidemia. NEAT had an area under the curve (AUC) of 0.931 (95% CI 0.844–0.978, p < 0.001). CONCLUSION: Dyslipidemia is an initiating signal in psoriasis pathogenesis that creates a state of chronic inflammation and oxidative stress. This state induces keratinocytes proliferation and release of NEAT with subsequent caspase-3 activation to counteract the proliferating cells. NEAT could be considered as a good diagnostic biomarker for psoriasis. GRAPHICAL ABSTRACT: [Image: see text] |
format | Online Article Text |
id | pubmed-10460715 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-104607152023-08-29 Dyslipidemia initiates keratinocytes proliferation through upregulation of lncRNA NEAT in psoriasis patients Mostafa, Abeer Sabry, Dina Aboraia, Nesreen Fawzy, Ahmed Abou-Elalla, Amany A. Mol Biol Rep Original Article BACKGROUND: Psoriasis is a chronic inflammatory immune-mediated and hyper proliferative skin disorder that has underlying genetic factors. Psoriasis can result from interaction of cytokines between keratinocytes and T-lymphocytes. NEAT is a lncRNA involved in immune modulation and has been previously studied in cancers. This study aims to clarify the unprecedented role of NEAT in psoriasis pathogenesis. METHODS: The study was conducted on 50 healthy control subjects and 50 psoriasis patients. Blood samples from all participants were collected for analysis of their lipid profile. qRT-PCR was done for lncRNA NEAT, TNF-α, VEGF genes expression. The levels of ROS and caspase-3 were estimated by ELISA. ROC analysis was done to detect the diagnostic value of lncRNA NEAT gene expression. RESULTS: Dyslipidemia is more prevalent among psoriasis patients. A significant up regulation in lncRNA NEAT, TNF-α, VEGF genes expression (p value˂0.001) in psoriasis patients in addition to significant increase in ROS and caspase-3 levels (p value˂0.001) in compare to controls. Additionally, a positive significant correlation between TNF-α, ROS, NEAT, caspase-3 and dyslipidemia. NEAT had an area under the curve (AUC) of 0.931 (95% CI 0.844–0.978, p < 0.001). CONCLUSION: Dyslipidemia is an initiating signal in psoriasis pathogenesis that creates a state of chronic inflammation and oxidative stress. This state induces keratinocytes proliferation and release of NEAT with subsequent caspase-3 activation to counteract the proliferating cells. NEAT could be considered as a good diagnostic biomarker for psoriasis. GRAPHICAL ABSTRACT: [Image: see text] Springer Netherlands 2023-08-02 2023 /pmc/articles/PMC10460715/ /pubmed/37531036 http://dx.doi.org/10.1007/s11033-023-08527-w Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Article Mostafa, Abeer Sabry, Dina Aboraia, Nesreen Fawzy, Ahmed Abou-Elalla, Amany A. Dyslipidemia initiates keratinocytes proliferation through upregulation of lncRNA NEAT in psoriasis patients |
title | Dyslipidemia initiates keratinocytes proliferation through upregulation of lncRNA NEAT in psoriasis patients |
title_full | Dyslipidemia initiates keratinocytes proliferation through upregulation of lncRNA NEAT in psoriasis patients |
title_fullStr | Dyslipidemia initiates keratinocytes proliferation through upregulation of lncRNA NEAT in psoriasis patients |
title_full_unstemmed | Dyslipidemia initiates keratinocytes proliferation through upregulation of lncRNA NEAT in psoriasis patients |
title_short | Dyslipidemia initiates keratinocytes proliferation through upregulation of lncRNA NEAT in psoriasis patients |
title_sort | dyslipidemia initiates keratinocytes proliferation through upregulation of lncrna neat in psoriasis patients |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10460715/ https://www.ncbi.nlm.nih.gov/pubmed/37531036 http://dx.doi.org/10.1007/s11033-023-08527-w |
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