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Cathepsin K‐Activated Probe for Fluoro‐Photoacoustic Imaging of Early Osteolytic Metastasis

Precise detection of early osteolytic metastases is crucial for their treatment but remains challenging in the clinic because of the limited sensitivity and specificity of traditional imaging techniques. Although fluorescence imaging offers attractive features for the diagnosis of osteolytic metasta...

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Autores principales: Song, Zhuorun, Miao, Jia, Miao, Minqian, Cheng, Baoliang, Li, Shenhua, Liu, Yinghua, Miao, Qingqing, Li, Qing, Gao, Mingyuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10460880/
https://www.ncbi.nlm.nih.gov/pubmed/37341286
http://dx.doi.org/10.1002/advs.202300217
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author Song, Zhuorun
Miao, Jia
Miao, Minqian
Cheng, Baoliang
Li, Shenhua
Liu, Yinghua
Miao, Qingqing
Li, Qing
Gao, Mingyuan
author_facet Song, Zhuorun
Miao, Jia
Miao, Minqian
Cheng, Baoliang
Li, Shenhua
Liu, Yinghua
Miao, Qingqing
Li, Qing
Gao, Mingyuan
author_sort Song, Zhuorun
collection PubMed
description Precise detection of early osteolytic metastases is crucial for their treatment but remains challenging in the clinic because of the limited sensitivity and specificity of traditional imaging techniques. Although fluorescence imaging offers attractive features for the diagnosis of osteolytic metastases, it is hampered by limited penetration depth. To address this issue, a fluoro‐photoacoustic dual‐modality imaging probe comprising a near‐infrared dye caged by a cathepsin K (CTSK)‐cleavable peptide sequence on one side and functionalized with osteophilic alendronate through a polyethylene glycol linker on the other side is reported. Through systematic in vitro and in vivo experiments, it is demonstrated that in response to CTSK, the probe generated both near‐infrared fluorescent and photoacoustic signals from bone metastatic regions, thus offering a potential strategy for detecting deep‐seated early osteolytic metastases.
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spelling pubmed-104608802023-08-29 Cathepsin K‐Activated Probe for Fluoro‐Photoacoustic Imaging of Early Osteolytic Metastasis Song, Zhuorun Miao, Jia Miao, Minqian Cheng, Baoliang Li, Shenhua Liu, Yinghua Miao, Qingqing Li, Qing Gao, Mingyuan Adv Sci (Weinh) Research Articles Precise detection of early osteolytic metastases is crucial for their treatment but remains challenging in the clinic because of the limited sensitivity and specificity of traditional imaging techniques. Although fluorescence imaging offers attractive features for the diagnosis of osteolytic metastases, it is hampered by limited penetration depth. To address this issue, a fluoro‐photoacoustic dual‐modality imaging probe comprising a near‐infrared dye caged by a cathepsin K (CTSK)‐cleavable peptide sequence on one side and functionalized with osteophilic alendronate through a polyethylene glycol linker on the other side is reported. Through systematic in vitro and in vivo experiments, it is demonstrated that in response to CTSK, the probe generated both near‐infrared fluorescent and photoacoustic signals from bone metastatic regions, thus offering a potential strategy for detecting deep‐seated early osteolytic metastases. John Wiley and Sons Inc. 2023-06-21 /pmc/articles/PMC10460880/ /pubmed/37341286 http://dx.doi.org/10.1002/advs.202300217 Text en © 2023 The Authors. Advanced Science published by Wiley‐VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Song, Zhuorun
Miao, Jia
Miao, Minqian
Cheng, Baoliang
Li, Shenhua
Liu, Yinghua
Miao, Qingqing
Li, Qing
Gao, Mingyuan
Cathepsin K‐Activated Probe for Fluoro‐Photoacoustic Imaging of Early Osteolytic Metastasis
title Cathepsin K‐Activated Probe for Fluoro‐Photoacoustic Imaging of Early Osteolytic Metastasis
title_full Cathepsin K‐Activated Probe for Fluoro‐Photoacoustic Imaging of Early Osteolytic Metastasis
title_fullStr Cathepsin K‐Activated Probe for Fluoro‐Photoacoustic Imaging of Early Osteolytic Metastasis
title_full_unstemmed Cathepsin K‐Activated Probe for Fluoro‐Photoacoustic Imaging of Early Osteolytic Metastasis
title_short Cathepsin K‐Activated Probe for Fluoro‐Photoacoustic Imaging of Early Osteolytic Metastasis
title_sort cathepsin k‐activated probe for fluoro‐photoacoustic imaging of early osteolytic metastasis
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10460880/
https://www.ncbi.nlm.nih.gov/pubmed/37341286
http://dx.doi.org/10.1002/advs.202300217
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