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Protocol for density gradient neutrophil isolation and flow cytometry-based characterization from human peripheral blood
Neutrophils are the first immune responders to bacterial or viral infection and play key roles in the host immune response; however, handling and investigating fresh neutrophils can be challenging. Here, we present a protocol for isolating neutrophils from the peripheral blood of healthy donors usin...
Autores principales: | Kuang, Yi, Parthasarathy, Upasana, Martinelli, Roberta |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10461015/ https://www.ncbi.nlm.nih.gov/pubmed/37590147 http://dx.doi.org/10.1016/j.xpro.2023.102497 |
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