Protocol for constructing GQD-PMO functionalized FET-biosensor for ultrasensitive exosomal miRNA detection

Here, we present a protocol for constructing an ultrasensitive biosensor for exosomal-miRNA detection. We describe steps for preparing graphene quantum dot-phosphorodiamidate morpholino oligomer hybrids, depositing them onto the reduced graphene oxide field surface, hybridizing analyte miRNA with th...

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Detalles Bibliográficos
Autores principales: Li, Kun, Liang, Chunzi, Pan, Yuling, Mei, Linghan, Zhang, Guo-Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10461019/
https://www.ncbi.nlm.nih.gov/pubmed/37590152
http://dx.doi.org/10.1016/j.xpro.2023.102516
Descripción
Sumario:Here, we present a protocol for constructing an ultrasensitive biosensor for exosomal-miRNA detection. We describe steps for preparing graphene quantum dot-phosphorodiamidate morpholino oligomer hybrids, depositing them onto the reduced graphene oxide field surface, hybridizing analyte miRNA with the sensor probe, and capturing and calculating electrical signals. We also detail procedures for optimizing biosensor construction and evaluating performance. By quantifying plasma exosomal miRNA21, this protocol can identify cancer patients from healthy individuals. For complete details on the use and execution of this protocol, please refer to Li et al.(1)

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