Changes in an Enzyme Ensemble During Catalysis Observed by High Resolution XFEL Crystallography

Enzymes populate ensembles of structures with intrinsically different catalytic proficiencies that are difficult to experimentally characterize. We use time-resolved mix-and-inject serial crystallography (MISC) at an X-ray free electron laser (XFEL) to observe catalysis in a designed mutant (G150T)...

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Detalles Bibliográficos
Autores principales: Smith, Nathan, Dasgupta, Medhanjali, Wych, David C., Dolamore, Cole, Sierra, Raymond G., Lisova, Stella, Marchany-Rivera, Darya, Cohen, Aina E., Boutet, Sébastien, Hunter, Mark S., Kupitz, Christopher, Poitevin, Frédéric, Moss, Frank R., Brewster, Aaron S., Sauter, Nicholas K., Young, Iris D., Wolff, Alexander M., Tiwari, Virendra K., Kumar, Nivesh, Berkowitz, David B., Hadt, Ryan G., Thompson, Michael C., Follmer, Alec H., Wall, Michael E., Wilson, Mark A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10462001/
https://www.ncbi.nlm.nih.gov/pubmed/37645800
http://dx.doi.org/10.1101/2023.08.15.553460

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