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Protocol to track the biosynthesis of cholesterol in cultured HCC cells using (13)C compound-specific stable isotopic tracers

Cholesterol biosynthesis supports proliferation and drives resistance to tyrosine kinase inhibitor (TKI) therapy in hepatocellular carcinoma (HCC). Here, we present a protocol for using stable isotopic tracers to track the biosynthesis of cholesterol in cultured HCC cells. We describe steps for cell...

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Detalles Bibliográficos
Autores principales:	Cybulski, Jonathan D., Leung, Kit Sum, Leung, Carmen Oi Ning, Baker, David M., Lee, Terence Kin Wah
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10462878/ https://www.ncbi.nlm.nih.gov/pubmed/37594893 http://dx.doi.org/10.1016/j.xpro.2023.102506

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