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Convergent evolution and B-cell recirculation in germinal centers in a human lymph node
Germinal centers (GCs) play a central role in generating an effective immune response against infectious pathogens, and failures in their regulating mechanisms can lead to the development of autoimmune diseases and cancer. Although previous works study experimental systems of the immune response wit...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Life Science Alliance LLC
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10462906/ https://www.ncbi.nlm.nih.gov/pubmed/37640448 http://dx.doi.org/10.26508/lsa.202301959 |
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author | Pelissier, Aurelien Stratigopoulou, Maria Donner, Naomi Dimitriadis, Evangelos Bende, Richard J Guikema, Jeroen E Rodriguez Martinez, Maria van Noesel, Carel JM |
author_facet | Pelissier, Aurelien Stratigopoulou, Maria Donner, Naomi Dimitriadis, Evangelos Bende, Richard J Guikema, Jeroen E Rodriguez Martinez, Maria van Noesel, Carel JM |
author_sort | Pelissier, Aurelien |
collection | PubMed |
description | Germinal centers (GCs) play a central role in generating an effective immune response against infectious pathogens, and failures in their regulating mechanisms can lead to the development of autoimmune diseases and cancer. Although previous works study experimental systems of the immune response with mouse models that are immunized with specific antigens, our study focused on a real-life situation, with an ongoing GC response in a human lymph node (LN) involving multiple asynchronized GCs reacting simultaneously to unknown antigens. We combined laser capture microdissection of individual GCs from human LN with next-generation repertoire sequencing to characterize individual GCs as distinct evolutionary spaces. In line with well-characterized GC responses in mice, elicited by immunization with model antigens, we observe a heterogeneous clonal diversity across individual GCs from the same human LN. Still, we identify shared clones in several individual GCs, and phylogenetic tree analysis combined with paratope modeling suggest the re-engagement and rediversification of B-cell clones across GCs and expanded clones exhibiting shared antigen responses across distinct GCs, indicating convergent evolution of the GCs. |
format | Online Article Text |
id | pubmed-10462906 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Life Science Alliance LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-104629062023-08-30 Convergent evolution and B-cell recirculation in germinal centers in a human lymph node Pelissier, Aurelien Stratigopoulou, Maria Donner, Naomi Dimitriadis, Evangelos Bende, Richard J Guikema, Jeroen E Rodriguez Martinez, Maria van Noesel, Carel JM Life Sci Alliance Research Articles Germinal centers (GCs) play a central role in generating an effective immune response against infectious pathogens, and failures in their regulating mechanisms can lead to the development of autoimmune diseases and cancer. Although previous works study experimental systems of the immune response with mouse models that are immunized with specific antigens, our study focused on a real-life situation, with an ongoing GC response in a human lymph node (LN) involving multiple asynchronized GCs reacting simultaneously to unknown antigens. We combined laser capture microdissection of individual GCs from human LN with next-generation repertoire sequencing to characterize individual GCs as distinct evolutionary spaces. In line with well-characterized GC responses in mice, elicited by immunization with model antigens, we observe a heterogeneous clonal diversity across individual GCs from the same human LN. Still, we identify shared clones in several individual GCs, and phylogenetic tree analysis combined with paratope modeling suggest the re-engagement and rediversification of B-cell clones across GCs and expanded clones exhibiting shared antigen responses across distinct GCs, indicating convergent evolution of the GCs. Life Science Alliance LLC 2023-08-28 /pmc/articles/PMC10462906/ /pubmed/37640448 http://dx.doi.org/10.26508/lsa.202301959 Text en © 2023 Pelissier et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Articles Pelissier, Aurelien Stratigopoulou, Maria Donner, Naomi Dimitriadis, Evangelos Bende, Richard J Guikema, Jeroen E Rodriguez Martinez, Maria van Noesel, Carel JM Convergent evolution and B-cell recirculation in germinal centers in a human lymph node |
title | Convergent evolution and B-cell recirculation in germinal centers in a human lymph node |
title_full | Convergent evolution and B-cell recirculation in germinal centers in a human lymph node |
title_fullStr | Convergent evolution and B-cell recirculation in germinal centers in a human lymph node |
title_full_unstemmed | Convergent evolution and B-cell recirculation in germinal centers in a human lymph node |
title_short | Convergent evolution and B-cell recirculation in germinal centers in a human lymph node |
title_sort | convergent evolution and b-cell recirculation in germinal centers in a human lymph node |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10462906/ https://www.ncbi.nlm.nih.gov/pubmed/37640448 http://dx.doi.org/10.26508/lsa.202301959 |
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