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Differential systemic decellularization in vivo to study molecular changes in each vasculature layer in murine models of disease

Vascular dysfunction underlies the onset and progression of many life-threatening diseases, highlighting the need for improved understanding of its molecular basis. Here, we present differential systemic decellularization in vivo (DISDIVO), a protocol that enables systemic and independent study of t...

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Detalles Bibliográficos
Autores principales: Gallart-Palau, Xavier, Lorca, Cristina, Mulet, María, Sánchez Milán, José Antonio, Lisa, Julia, Ngan, SoFong Cam, Iyappan, Ranjith, Katoueezadeh, Maryam, Serra, Aida, Sze, Siu Kwan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10463255/
https://www.ncbi.nlm.nih.gov/pubmed/37624701
http://dx.doi.org/10.1016/j.xpro.2023.102524
Descripción
Sumario:Vascular dysfunction underlies the onset and progression of many life-threatening diseases, highlighting the need for improved understanding of its molecular basis. Here, we present differential systemic decellularization in vivo (DISDIVO), a protocol that enables systemic and independent study of the molecular changes in each vasculature layer in murine models of disease. We describe steps for anesthesia, perfusion surgery, and exsanguination. We then detail detachment and collection of glycocalyx and decellularization and collection of both endothelial and smooth muscle cells. For complete details on the use and execution of this protocol, please refer to Serra et al., Gallart-Palau et al., and Vinaiphat et al.(1)(,)(2)(,)(3)