Protocol to locally express cxcl12a during zebrafish olfactory organ development by combining IR-LEGO with live imaging

Temporal and spatial regulation of gene expression is crucial for proper embryonic development. Infrared laser-evoked gene operator (IR-LEGO) can provide information for various developmental processes. Here, we present a protocol to locally express cxcl12a during zebrafish olfactory organ developme...

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Detalles Bibliográficos
Autores principales: Zilliox, Marie, Tillement, Vanessa, Mangeat, Thomas, Polès, Sophie, Blader, Patrick, Batut, Julie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10463262/
https://www.ncbi.nlm.nih.gov/pubmed/37624700
http://dx.doi.org/10.1016/j.xpro.2023.102538
Descripción
Sumario:Temporal and spatial regulation of gene expression is crucial for proper embryonic development. Infrared laser-evoked gene operator (IR-LEGO) can provide information for various developmental processes. Here, we present a protocol to locally express cxcl12a during zebrafish olfactory organ development(1) using a combination of IR-LEGO and live imaging. We describe steps for implementing IR-LEGO, biological sample preparation, live imaging, data collection, and analysis. This protocol can be applied to virtually any genetically modified experimental organism.

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