Protocol to locally express cxcl12a during zebrafish olfactory organ development by combining IR-LEGO with live imaging

Temporal and spatial regulation of gene expression is crucial for proper embryonic development. Infrared laser-evoked gene operator (IR-LEGO) can provide information for various developmental processes. Here, we present a protocol to locally express cxcl12a during zebrafish olfactory organ developme...

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Autores principales: Zilliox, Marie, Tillement, Vanessa, Mangeat, Thomas, Polès, Sophie, Blader, Patrick, Batut, Julie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10463262/
https://www.ncbi.nlm.nih.gov/pubmed/37624700
http://dx.doi.org/10.1016/j.xpro.2023.102538
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author Zilliox, Marie
Tillement, Vanessa
Mangeat, Thomas
Polès, Sophie
Blader, Patrick
Batut, Julie
author_facet Zilliox, Marie
Tillement, Vanessa
Mangeat, Thomas
Polès, Sophie
Blader, Patrick
Batut, Julie
author_sort Zilliox, Marie
collection PubMed
description Temporal and spatial regulation of gene expression is crucial for proper embryonic development. Infrared laser-evoked gene operator (IR-LEGO) can provide information for various developmental processes. Here, we present a protocol to locally express cxcl12a during zebrafish olfactory organ development(1) using a combination of IR-LEGO and live imaging. We describe steps for implementing IR-LEGO, biological sample preparation, live imaging, data collection, and analysis. This protocol can be applied to virtually any genetically modified experimental organism.
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spelling pubmed-104632622023-08-30 Protocol to locally express cxcl12a during zebrafish olfactory organ development by combining IR-LEGO with live imaging Zilliox, Marie Tillement, Vanessa Mangeat, Thomas Polès, Sophie Blader, Patrick Batut, Julie STAR Protoc Protocol Temporal and spatial regulation of gene expression is crucial for proper embryonic development. Infrared laser-evoked gene operator (IR-LEGO) can provide information for various developmental processes. Here, we present a protocol to locally express cxcl12a during zebrafish olfactory organ development(1) using a combination of IR-LEGO and live imaging. We describe steps for implementing IR-LEGO, biological sample preparation, live imaging, data collection, and analysis. This protocol can be applied to virtually any genetically modified experimental organism. Elsevier 2023-08-23 /pmc/articles/PMC10463262/ /pubmed/37624700 http://dx.doi.org/10.1016/j.xpro.2023.102538 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Zilliox, Marie
Tillement, Vanessa
Mangeat, Thomas
Polès, Sophie
Blader, Patrick
Batut, Julie
Protocol to locally express cxcl12a during zebrafish olfactory organ development by combining IR-LEGO with live imaging
title Protocol to locally express cxcl12a during zebrafish olfactory organ development by combining IR-LEGO with live imaging
title_full Protocol to locally express cxcl12a during zebrafish olfactory organ development by combining IR-LEGO with live imaging
title_fullStr Protocol to locally express cxcl12a during zebrafish olfactory organ development by combining IR-LEGO with live imaging
title_full_unstemmed Protocol to locally express cxcl12a during zebrafish olfactory organ development by combining IR-LEGO with live imaging
title_short Protocol to locally express cxcl12a during zebrafish olfactory organ development by combining IR-LEGO with live imaging
title_sort protocol to locally express cxcl12a during zebrafish olfactory organ development by combining ir-lego with live imaging
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10463262/
https://www.ncbi.nlm.nih.gov/pubmed/37624700
http://dx.doi.org/10.1016/j.xpro.2023.102538
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