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Rapid Direct Detection of SARS-CoV-2 Aerosols in Exhaled Breath at the Point of Care

[Image: see text] Airborne transmission via virus-laden aerosols is a dominant route for the transmission of respiratory diseases, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Direct, non-invasive screening of respiratory virus aerosols in patients has been a long-standing...

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Autores principales: Ghumra, Dishit P., Shetty, Nishit, McBrearty, Kevin R., Puthussery, Joseph V., Sumlin, Benjamin J., Gardiner, Woodrow D., Doherty, Brookelyn M., Magrecki, Jordan P., Brody, David L., Esparza, Thomas J., O’Halloran, Jane A., Presti, Rachel M., Bricker, Traci L., Boon, Adrianus C. M., Yuede, Carla M., Cirrito, John R., Chakrabarty, Rajan K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10463275/
https://www.ncbi.nlm.nih.gov/pubmed/37498298
http://dx.doi.org/10.1021/acssensors.3c00512
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author Ghumra, Dishit P.
Shetty, Nishit
McBrearty, Kevin R.
Puthussery, Joseph V.
Sumlin, Benjamin J.
Gardiner, Woodrow D.
Doherty, Brookelyn M.
Magrecki, Jordan P.
Brody, David L.
Esparza, Thomas J.
O’Halloran, Jane A.
Presti, Rachel M.
Bricker, Traci L.
Boon, Adrianus C. M.
Yuede, Carla M.
Cirrito, John R.
Chakrabarty, Rajan K.
author_facet Ghumra, Dishit P.
Shetty, Nishit
McBrearty, Kevin R.
Puthussery, Joseph V.
Sumlin, Benjamin J.
Gardiner, Woodrow D.
Doherty, Brookelyn M.
Magrecki, Jordan P.
Brody, David L.
Esparza, Thomas J.
O’Halloran, Jane A.
Presti, Rachel M.
Bricker, Traci L.
Boon, Adrianus C. M.
Yuede, Carla M.
Cirrito, John R.
Chakrabarty, Rajan K.
author_sort Ghumra, Dishit P.
collection PubMed
description [Image: see text] Airborne transmission via virus-laden aerosols is a dominant route for the transmission of respiratory diseases, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Direct, non-invasive screening of respiratory virus aerosols in patients has been a long-standing technical challenge. Here, we introduce a point-of-care testing platform that directly detects SARS-CoV-2 aerosols in as little as two exhaled breaths of patients and provides results in under 60 s. It integrates a hand-held breath aerosol collector and a llama-derived, SARS-CoV-2 spike-protein specific nanobody bound to an ultrasensitive micro-immunoelectrode biosensor, which detects the oxidation of tyrosine amino acids present in SARS-CoV-2 viral particles. Laboratory and clinical trial results were within 20% of those obtained using standard testing methods. Importantly, the electrochemical biosensor directly detects the virus itself, as opposed to a surrogate or signature of the virus, and is sensitive to as little as 10 viral particles in a sample. Our platform holds the potential to be adapted for multiplexed detection of different respiratory viruses. It provides a rapid and non-invasive alternative to conventional viral diagnostics.
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spelling pubmed-104632752023-08-30 Rapid Direct Detection of SARS-CoV-2 Aerosols in Exhaled Breath at the Point of Care Ghumra, Dishit P. Shetty, Nishit McBrearty, Kevin R. Puthussery, Joseph V. Sumlin, Benjamin J. Gardiner, Woodrow D. Doherty, Brookelyn M. Magrecki, Jordan P. Brody, David L. Esparza, Thomas J. O’Halloran, Jane A. Presti, Rachel M. Bricker, Traci L. Boon, Adrianus C. M. Yuede, Carla M. Cirrito, John R. Chakrabarty, Rajan K. ACS Sens [Image: see text] Airborne transmission via virus-laden aerosols is a dominant route for the transmission of respiratory diseases, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Direct, non-invasive screening of respiratory virus aerosols in patients has been a long-standing technical challenge. Here, we introduce a point-of-care testing platform that directly detects SARS-CoV-2 aerosols in as little as two exhaled breaths of patients and provides results in under 60 s. It integrates a hand-held breath aerosol collector and a llama-derived, SARS-CoV-2 spike-protein specific nanobody bound to an ultrasensitive micro-immunoelectrode biosensor, which detects the oxidation of tyrosine amino acids present in SARS-CoV-2 viral particles. Laboratory and clinical trial results were within 20% of those obtained using standard testing methods. Importantly, the electrochemical biosensor directly detects the virus itself, as opposed to a surrogate or signature of the virus, and is sensitive to as little as 10 viral particles in a sample. Our platform holds the potential to be adapted for multiplexed detection of different respiratory viruses. It provides a rapid and non-invasive alternative to conventional viral diagnostics. American Chemical Society 2023-07-27 /pmc/articles/PMC10463275/ /pubmed/37498298 http://dx.doi.org/10.1021/acssensors.3c00512 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Ghumra, Dishit P.
Shetty, Nishit
McBrearty, Kevin R.
Puthussery, Joseph V.
Sumlin, Benjamin J.
Gardiner, Woodrow D.
Doherty, Brookelyn M.
Magrecki, Jordan P.
Brody, David L.
Esparza, Thomas J.
O’Halloran, Jane A.
Presti, Rachel M.
Bricker, Traci L.
Boon, Adrianus C. M.
Yuede, Carla M.
Cirrito, John R.
Chakrabarty, Rajan K.
Rapid Direct Detection of SARS-CoV-2 Aerosols in Exhaled Breath at the Point of Care
title Rapid Direct Detection of SARS-CoV-2 Aerosols in Exhaled Breath at the Point of Care
title_full Rapid Direct Detection of SARS-CoV-2 Aerosols in Exhaled Breath at the Point of Care
title_fullStr Rapid Direct Detection of SARS-CoV-2 Aerosols in Exhaled Breath at the Point of Care
title_full_unstemmed Rapid Direct Detection of SARS-CoV-2 Aerosols in Exhaled Breath at the Point of Care
title_short Rapid Direct Detection of SARS-CoV-2 Aerosols in Exhaled Breath at the Point of Care
title_sort rapid direct detection of sars-cov-2 aerosols in exhaled breath at the point of care
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10463275/
https://www.ncbi.nlm.nih.gov/pubmed/37498298
http://dx.doi.org/10.1021/acssensors.3c00512
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