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Rapid Direct Detection of SARS-CoV-2 Aerosols in Exhaled Breath at the Point of Care
[Image: see text] Airborne transmission via virus-laden aerosols is a dominant route for the transmission of respiratory diseases, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Direct, non-invasive screening of respiratory virus aerosols in patients has been a long-standing...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10463275/ https://www.ncbi.nlm.nih.gov/pubmed/37498298 http://dx.doi.org/10.1021/acssensors.3c00512 |
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author | Ghumra, Dishit P. Shetty, Nishit McBrearty, Kevin R. Puthussery, Joseph V. Sumlin, Benjamin J. Gardiner, Woodrow D. Doherty, Brookelyn M. Magrecki, Jordan P. Brody, David L. Esparza, Thomas J. O’Halloran, Jane A. Presti, Rachel M. Bricker, Traci L. Boon, Adrianus C. M. Yuede, Carla M. Cirrito, John R. Chakrabarty, Rajan K. |
author_facet | Ghumra, Dishit P. Shetty, Nishit McBrearty, Kevin R. Puthussery, Joseph V. Sumlin, Benjamin J. Gardiner, Woodrow D. Doherty, Brookelyn M. Magrecki, Jordan P. Brody, David L. Esparza, Thomas J. O’Halloran, Jane A. Presti, Rachel M. Bricker, Traci L. Boon, Adrianus C. M. Yuede, Carla M. Cirrito, John R. Chakrabarty, Rajan K. |
author_sort | Ghumra, Dishit P. |
collection | PubMed |
description | [Image: see text] Airborne transmission via virus-laden aerosols is a dominant route for the transmission of respiratory diseases, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Direct, non-invasive screening of respiratory virus aerosols in patients has been a long-standing technical challenge. Here, we introduce a point-of-care testing platform that directly detects SARS-CoV-2 aerosols in as little as two exhaled breaths of patients and provides results in under 60 s. It integrates a hand-held breath aerosol collector and a llama-derived, SARS-CoV-2 spike-protein specific nanobody bound to an ultrasensitive micro-immunoelectrode biosensor, which detects the oxidation of tyrosine amino acids present in SARS-CoV-2 viral particles. Laboratory and clinical trial results were within 20% of those obtained using standard testing methods. Importantly, the electrochemical biosensor directly detects the virus itself, as opposed to a surrogate or signature of the virus, and is sensitive to as little as 10 viral particles in a sample. Our platform holds the potential to be adapted for multiplexed detection of different respiratory viruses. It provides a rapid and non-invasive alternative to conventional viral diagnostics. |
format | Online Article Text |
id | pubmed-10463275 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-104632752023-08-30 Rapid Direct Detection of SARS-CoV-2 Aerosols in Exhaled Breath at the Point of Care Ghumra, Dishit P. Shetty, Nishit McBrearty, Kevin R. Puthussery, Joseph V. Sumlin, Benjamin J. Gardiner, Woodrow D. Doherty, Brookelyn M. Magrecki, Jordan P. Brody, David L. Esparza, Thomas J. O’Halloran, Jane A. Presti, Rachel M. Bricker, Traci L. Boon, Adrianus C. M. Yuede, Carla M. Cirrito, John R. Chakrabarty, Rajan K. ACS Sens [Image: see text] Airborne transmission via virus-laden aerosols is a dominant route for the transmission of respiratory diseases, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Direct, non-invasive screening of respiratory virus aerosols in patients has been a long-standing technical challenge. Here, we introduce a point-of-care testing platform that directly detects SARS-CoV-2 aerosols in as little as two exhaled breaths of patients and provides results in under 60 s. It integrates a hand-held breath aerosol collector and a llama-derived, SARS-CoV-2 spike-protein specific nanobody bound to an ultrasensitive micro-immunoelectrode biosensor, which detects the oxidation of tyrosine amino acids present in SARS-CoV-2 viral particles. Laboratory and clinical trial results were within 20% of those obtained using standard testing methods. Importantly, the electrochemical biosensor directly detects the virus itself, as opposed to a surrogate or signature of the virus, and is sensitive to as little as 10 viral particles in a sample. Our platform holds the potential to be adapted for multiplexed detection of different respiratory viruses. It provides a rapid and non-invasive alternative to conventional viral diagnostics. American Chemical Society 2023-07-27 /pmc/articles/PMC10463275/ /pubmed/37498298 http://dx.doi.org/10.1021/acssensors.3c00512 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Ghumra, Dishit P. Shetty, Nishit McBrearty, Kevin R. Puthussery, Joseph V. Sumlin, Benjamin J. Gardiner, Woodrow D. Doherty, Brookelyn M. Magrecki, Jordan P. Brody, David L. Esparza, Thomas J. O’Halloran, Jane A. Presti, Rachel M. Bricker, Traci L. Boon, Adrianus C. M. Yuede, Carla M. Cirrito, John R. Chakrabarty, Rajan K. Rapid Direct Detection of SARS-CoV-2 Aerosols in Exhaled Breath at the Point of Care |
title | Rapid Direct
Detection of SARS-CoV-2 Aerosols
in Exhaled Breath at the Point of Care |
title_full | Rapid Direct
Detection of SARS-CoV-2 Aerosols
in Exhaled Breath at the Point of Care |
title_fullStr | Rapid Direct
Detection of SARS-CoV-2 Aerosols
in Exhaled Breath at the Point of Care |
title_full_unstemmed | Rapid Direct
Detection of SARS-CoV-2 Aerosols
in Exhaled Breath at the Point of Care |
title_short | Rapid Direct
Detection of SARS-CoV-2 Aerosols
in Exhaled Breath at the Point of Care |
title_sort | rapid direct
detection of sars-cov-2 aerosols
in exhaled breath at the point of care |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10463275/ https://www.ncbi.nlm.nih.gov/pubmed/37498298 http://dx.doi.org/10.1021/acssensors.3c00512 |
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