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The protective role of chicken cathelicidin-1 against Streptococcus suis serotype 2 in vitro and in vivo

Streptococcus suis serotype 2 (SS2) is an important zoonotic pathogen with the characteristics of high mortality and morbidity, which brings great challenges to prevent and control epidemic disease in the swine industry. Cathelicidins (CATH) are antimicrobial peptides with antimicrobial and immunomo...

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Autores principales: Lu, Yi, Xiang, Fa, Xu, Liuyi, Tian, Hongliang, Tao, Qi, Jia, Kaixiang, Yin, Hang, Ye, Chao, Fang, Rendong, Peng, Lianci
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10463303/
https://www.ncbi.nlm.nih.gov/pubmed/37605242
http://dx.doi.org/10.1186/s13567-023-01199-1
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author Lu, Yi
Xiang, Fa
Xu, Liuyi
Tian, Hongliang
Tao, Qi
Jia, Kaixiang
Yin, Hang
Ye, Chao
Fang, Rendong
Peng, Lianci
author_facet Lu, Yi
Xiang, Fa
Xu, Liuyi
Tian, Hongliang
Tao, Qi
Jia, Kaixiang
Yin, Hang
Ye, Chao
Fang, Rendong
Peng, Lianci
author_sort Lu, Yi
collection PubMed
description Streptococcus suis serotype 2 (SS2) is an important zoonotic pathogen with the characteristics of high mortality and morbidity, which brings great challenges to prevent and control epidemic disease in the swine industry. Cathelicidins (CATH) are antimicrobial peptides with antimicrobial and immunomodulatory activities. In this study, bactericidal and anti-inflammatory effects of chicken cathelicidin-1 (CATH-1) were investigated in vitro and in vivo against SS2 infection. The results show that CATH-1 exhibited a better bactericidal effect compared to other species’ cathelicidins including chickens (CATH-2, -3, and -B1), mice (CRAMP) and pigs (PMAP-36 and PR-39), which rapidly killed bacteria in 20 min by a time-killing curve assay. Furthermore, CATH-1 destroyed the bacterial morphology and affected bacterial ultrastructure as observed under electron microscopy. Moreover, CATH-1 antibacterial activity in vivo shows that CATH-1 increased survival rate of SS2-infected mice by 60% and significantly reduced the bacterial load in the lungs, liver, spleen, blood, and peritoneal lavage as well as the release of SS2-induced inflammatory cytokines including IL-1α, IL-1β, IL-12, and IL-18. Importantly, CATH-1 did not show severe histopathological changes in mice. Further studies on the mechanism of anti-inflammatory activity show that CATH-1 not only reduced the inflammatory response through direct neutralization, but also by regulating the TLR2/4/NF-κB/ERK pathway. This study provides a scientific basis for the research and development of antimicrobial peptides as new antimicrobial agents. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13567-023-01199-1.
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spelling pubmed-104633032023-08-30 The protective role of chicken cathelicidin-1 against Streptococcus suis serotype 2 in vitro and in vivo Lu, Yi Xiang, Fa Xu, Liuyi Tian, Hongliang Tao, Qi Jia, Kaixiang Yin, Hang Ye, Chao Fang, Rendong Peng, Lianci Vet Res Research Article Streptococcus suis serotype 2 (SS2) is an important zoonotic pathogen with the characteristics of high mortality and morbidity, which brings great challenges to prevent and control epidemic disease in the swine industry. Cathelicidins (CATH) are antimicrobial peptides with antimicrobial and immunomodulatory activities. In this study, bactericidal and anti-inflammatory effects of chicken cathelicidin-1 (CATH-1) were investigated in vitro and in vivo against SS2 infection. The results show that CATH-1 exhibited a better bactericidal effect compared to other species’ cathelicidins including chickens (CATH-2, -3, and -B1), mice (CRAMP) and pigs (PMAP-36 and PR-39), which rapidly killed bacteria in 20 min by a time-killing curve assay. Furthermore, CATH-1 destroyed the bacterial morphology and affected bacterial ultrastructure as observed under electron microscopy. Moreover, CATH-1 antibacterial activity in vivo shows that CATH-1 increased survival rate of SS2-infected mice by 60% and significantly reduced the bacterial load in the lungs, liver, spleen, blood, and peritoneal lavage as well as the release of SS2-induced inflammatory cytokines including IL-1α, IL-1β, IL-12, and IL-18. Importantly, CATH-1 did not show severe histopathological changes in mice. Further studies on the mechanism of anti-inflammatory activity show that CATH-1 not only reduced the inflammatory response through direct neutralization, but also by regulating the TLR2/4/NF-κB/ERK pathway. This study provides a scientific basis for the research and development of antimicrobial peptides as new antimicrobial agents. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13567-023-01199-1. BioMed Central 2023-08-21 2023 /pmc/articles/PMC10463303/ /pubmed/37605242 http://dx.doi.org/10.1186/s13567-023-01199-1 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Lu, Yi
Xiang, Fa
Xu, Liuyi
Tian, Hongliang
Tao, Qi
Jia, Kaixiang
Yin, Hang
Ye, Chao
Fang, Rendong
Peng, Lianci
The protective role of chicken cathelicidin-1 against Streptococcus suis serotype 2 in vitro and in vivo
title The protective role of chicken cathelicidin-1 against Streptococcus suis serotype 2 in vitro and in vivo
title_full The protective role of chicken cathelicidin-1 against Streptococcus suis serotype 2 in vitro and in vivo
title_fullStr The protective role of chicken cathelicidin-1 against Streptococcus suis serotype 2 in vitro and in vivo
title_full_unstemmed The protective role of chicken cathelicidin-1 against Streptococcus suis serotype 2 in vitro and in vivo
title_short The protective role of chicken cathelicidin-1 against Streptococcus suis serotype 2 in vitro and in vivo
title_sort protective role of chicken cathelicidin-1 against streptococcus suis serotype 2 in vitro and in vivo
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10463303/
https://www.ncbi.nlm.nih.gov/pubmed/37605242
http://dx.doi.org/10.1186/s13567-023-01199-1
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