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Effect of betanin synthesis on photosynthesis and tyrosine metabolism in transgenic carrot
BACKGROUND: Betalain is a natural pigment with important nutritional value and broad application prospects. Previously, we produced betanin biosynthesis transgenic carrots via expressing optimized genes CYP76AD1S, cDOPA5GTS and DODA1S. Betanin can accumulate throughout the whole transgenic carrots....
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10464428/ https://www.ncbi.nlm.nih.gov/pubmed/37620775 http://dx.doi.org/10.1186/s12870-023-04383-9 |
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author | Wang, Bo Wang, Ya-Hui Deng, Yuan-Jie Yao, Quan-Hong Xiong, Ai-Sheng |
author_facet | Wang, Bo Wang, Ya-Hui Deng, Yuan-Jie Yao, Quan-Hong Xiong, Ai-Sheng |
author_sort | Wang, Bo |
collection | PubMed |
description | BACKGROUND: Betalain is a natural pigment with important nutritional value and broad application prospects. Previously, we produced betanin biosynthesis transgenic carrots via expressing optimized genes CYP76AD1S, cDOPA5GTS and DODA1S. Betanin can accumulate throughout the whole transgenic carrots. But the effects of betanin accumulation on the metabolism of transgenic plants and whether it produces unexpected effects are still unclear. RESULTS: The accumulation of betanin in leaves can significantly improve its antioxidant capacity and induce a decrease of chlorophyll content. Transcriptome and metabolomics analysis showed that 14.0% of genes and 33.1% of metabolites were significantly different, and metabolic pathways related to photosynthesis and tyrosine metabolism were markedly altered. Combined analysis showed that phenylpropane biosynthesis pathway significantly enriched the differentially expressed genes and significantly altered metabolites. CONCLUSIONS: Results showed that the metabolic status was significantly altered between transgenic and non-transgenic carrots, especially the photosynthesis and tyrosine metabolism. The extra consumption of tyrosine and accumulation of betanin might be the leading causes. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-023-04383-9. |
format | Online Article Text |
id | pubmed-10464428 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-104644282023-08-30 Effect of betanin synthesis on photosynthesis and tyrosine metabolism in transgenic carrot Wang, Bo Wang, Ya-Hui Deng, Yuan-Jie Yao, Quan-Hong Xiong, Ai-Sheng BMC Plant Biol Research BACKGROUND: Betalain is a natural pigment with important nutritional value and broad application prospects. Previously, we produced betanin biosynthesis transgenic carrots via expressing optimized genes CYP76AD1S, cDOPA5GTS and DODA1S. Betanin can accumulate throughout the whole transgenic carrots. But the effects of betanin accumulation on the metabolism of transgenic plants and whether it produces unexpected effects are still unclear. RESULTS: The accumulation of betanin in leaves can significantly improve its antioxidant capacity and induce a decrease of chlorophyll content. Transcriptome and metabolomics analysis showed that 14.0% of genes and 33.1% of metabolites were significantly different, and metabolic pathways related to photosynthesis and tyrosine metabolism were markedly altered. Combined analysis showed that phenylpropane biosynthesis pathway significantly enriched the differentially expressed genes and significantly altered metabolites. CONCLUSIONS: Results showed that the metabolic status was significantly altered between transgenic and non-transgenic carrots, especially the photosynthesis and tyrosine metabolism. The extra consumption of tyrosine and accumulation of betanin might be the leading causes. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-023-04383-9. BioMed Central 2023-08-24 /pmc/articles/PMC10464428/ /pubmed/37620775 http://dx.doi.org/10.1186/s12870-023-04383-9 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Wang, Bo Wang, Ya-Hui Deng, Yuan-Jie Yao, Quan-Hong Xiong, Ai-Sheng Effect of betanin synthesis on photosynthesis and tyrosine metabolism in transgenic carrot |
title | Effect of betanin synthesis on photosynthesis and tyrosine metabolism in transgenic carrot |
title_full | Effect of betanin synthesis on photosynthesis and tyrosine metabolism in transgenic carrot |
title_fullStr | Effect of betanin synthesis on photosynthesis and tyrosine metabolism in transgenic carrot |
title_full_unstemmed | Effect of betanin synthesis on photosynthesis and tyrosine metabolism in transgenic carrot |
title_short | Effect of betanin synthesis on photosynthesis and tyrosine metabolism in transgenic carrot |
title_sort | effect of betanin synthesis on photosynthesis and tyrosine metabolism in transgenic carrot |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10464428/ https://www.ncbi.nlm.nih.gov/pubmed/37620775 http://dx.doi.org/10.1186/s12870-023-04383-9 |
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