Cargando…

Dynamic changes in the levels of metabolites and endogenous hormones during the germination of Zanthoxylum nitidum (Roxb.) DC. Seeds

Accumulating experimental data have shown that endogenous hormones play important roles in regulating seed dormancy and germination. Zanthoxylum nitidum is a medicinal plant that propagates via seeds, which require a long dormancy period for normal germination, and complex changes in metabolites occ...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Liang, Zhu, Yanxia, Jiang, Jianping, Tan, Guiyu, Ma, Qing, Zhang, Hongsheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10464536/
https://www.ncbi.nlm.nih.gov/pubmed/37639213
http://dx.doi.org/10.1080/15592324.2023.2251750
_version_ 1785098490759610368
author Wang, Liang
Zhu, Yanxia
Jiang, Jianping
Tan, Guiyu
Ma, Qing
Zhang, Hongsheng
author_facet Wang, Liang
Zhu, Yanxia
Jiang, Jianping
Tan, Guiyu
Ma, Qing
Zhang, Hongsheng
author_sort Wang, Liang
collection PubMed
description Accumulating experimental data have shown that endogenous hormones play important roles in regulating seed dormancy and germination. Zanthoxylum nitidum is a medicinal plant that propagates via seeds, which require a long dormancy period for normal germination, and complex changes in metabolites occur during the germination process. However, the regulatory network of endogenous hormones and metabolites during the germination of Z. nitidum seeds remains unclear. This study investigated the dynamic changes in the levels of metabolites and endogenous hormones during the germination of Z. nitidum seeds. The results revealed an increase in the levels of gibberellin 3 (GA(3)), 12-oxophytodienoic acid (OPDA), 1-aminocyclopropane-1-carboxylic acid (ACC) and trans-zeatin (TZ) and decrease in the levels of abscisic acid (ABA), jasmonic acid (JA), N-[(-)-jasmonoyl]-(S)-isoleucine (JA-Ile) and trans-zeatin riboside (TZR). Overall, 112 differential metabolites (DAMs) were screened from 3 seed samples (Sa, Sb and Sc), most of which are related to primary metabolism. A total of 16 DAMs (including 3 monosaccharides, 3 phosphate lipids, 3 carboxylic acids, 1 amino acid, 2 pyrimidines, and 4 nucleotides) were identified in the three sample comparison pairs (Sa vs Sb, Sa vs Sc, and Sb vs Sc); these DAMs were significantly enriched in purine metabolism; glycerophospholipid metabolism, citrate cycle (TCA cycle), alanine, aspartate and glutamate metabolism and pyruvate metabolism. OPDA, ACC and GAs were significantly positively correlated with upregulated metabolites, whereas ABA and JA were significantly positively correlated with downregulated metabolites. Finally, a hypothetical metabolic network of endogenous hormones that regulate seed germination was constructed. This study deepens our understanding of the importance of endogenous hormonal profiles that mediate seed germination.
format Online
Article
Text
id pubmed-10464536
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Taylor & Francis
record_format MEDLINE/PubMed
spelling pubmed-104645362023-08-30 Dynamic changes in the levels of metabolites and endogenous hormones during the germination of Zanthoxylum nitidum (Roxb.) DC. Seeds Wang, Liang Zhu, Yanxia Jiang, Jianping Tan, Guiyu Ma, Qing Zhang, Hongsheng Plant Signal Behav Research Paper Accumulating experimental data have shown that endogenous hormones play important roles in regulating seed dormancy and germination. Zanthoxylum nitidum is a medicinal plant that propagates via seeds, which require a long dormancy period for normal germination, and complex changes in metabolites occur during the germination process. However, the regulatory network of endogenous hormones and metabolites during the germination of Z. nitidum seeds remains unclear. This study investigated the dynamic changes in the levels of metabolites and endogenous hormones during the germination of Z. nitidum seeds. The results revealed an increase in the levels of gibberellin 3 (GA(3)), 12-oxophytodienoic acid (OPDA), 1-aminocyclopropane-1-carboxylic acid (ACC) and trans-zeatin (TZ) and decrease in the levels of abscisic acid (ABA), jasmonic acid (JA), N-[(-)-jasmonoyl]-(S)-isoleucine (JA-Ile) and trans-zeatin riboside (TZR). Overall, 112 differential metabolites (DAMs) were screened from 3 seed samples (Sa, Sb and Sc), most of which are related to primary metabolism. A total of 16 DAMs (including 3 monosaccharides, 3 phosphate lipids, 3 carboxylic acids, 1 amino acid, 2 pyrimidines, and 4 nucleotides) were identified in the three sample comparison pairs (Sa vs Sb, Sa vs Sc, and Sb vs Sc); these DAMs were significantly enriched in purine metabolism; glycerophospholipid metabolism, citrate cycle (TCA cycle), alanine, aspartate and glutamate metabolism and pyruvate metabolism. OPDA, ACC and GAs were significantly positively correlated with upregulated metabolites, whereas ABA and JA were significantly positively correlated with downregulated metabolites. Finally, a hypothetical metabolic network of endogenous hormones that regulate seed germination was constructed. This study deepens our understanding of the importance of endogenous hormonal profiles that mediate seed germination. Taylor & Francis 2023-08-28 /pmc/articles/PMC10464536/ /pubmed/37639213 http://dx.doi.org/10.1080/15592324.2023.2251750 Text en © 2023 The Author(s). Published with license by Taylor & Francis Group, LLC. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.
spellingShingle Research Paper
Wang, Liang
Zhu, Yanxia
Jiang, Jianping
Tan, Guiyu
Ma, Qing
Zhang, Hongsheng
Dynamic changes in the levels of metabolites and endogenous hormones during the germination of Zanthoxylum nitidum (Roxb.) DC. Seeds
title Dynamic changes in the levels of metabolites and endogenous hormones during the germination of Zanthoxylum nitidum (Roxb.) DC. Seeds
title_full Dynamic changes in the levels of metabolites and endogenous hormones during the germination of Zanthoxylum nitidum (Roxb.) DC. Seeds
title_fullStr Dynamic changes in the levels of metabolites and endogenous hormones during the germination of Zanthoxylum nitidum (Roxb.) DC. Seeds
title_full_unstemmed Dynamic changes in the levels of metabolites and endogenous hormones during the germination of Zanthoxylum nitidum (Roxb.) DC. Seeds
title_short Dynamic changes in the levels of metabolites and endogenous hormones during the germination of Zanthoxylum nitidum (Roxb.) DC. Seeds
title_sort dynamic changes in the levels of metabolites and endogenous hormones during the germination of zanthoxylum nitidum (roxb.) dc. seeds
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10464536/
https://www.ncbi.nlm.nih.gov/pubmed/37639213
http://dx.doi.org/10.1080/15592324.2023.2251750
work_keys_str_mv AT wangliang dynamicchangesinthelevelsofmetabolitesandendogenoushormonesduringthegerminationofzanthoxylumnitidumroxbdcseeds
AT zhuyanxia dynamicchangesinthelevelsofmetabolitesandendogenoushormonesduringthegerminationofzanthoxylumnitidumroxbdcseeds
AT jiangjianping dynamicchangesinthelevelsofmetabolitesandendogenoushormonesduringthegerminationofzanthoxylumnitidumroxbdcseeds
AT tanguiyu dynamicchangesinthelevelsofmetabolitesandendogenoushormonesduringthegerminationofzanthoxylumnitidumroxbdcseeds
AT maqing dynamicchangesinthelevelsofmetabolitesandendogenoushormonesduringthegerminationofzanthoxylumnitidumroxbdcseeds
AT zhanghongsheng dynamicchangesinthelevelsofmetabolitesandendogenoushormonesduringthegerminationofzanthoxylumnitidumroxbdcseeds