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Impact of the carbon flux regulator protein pirC on ethanol production in engineered cyanobacteria

Future sustainable energy production can be achieved using mass cultures of photoautotrophic microorganisms such as cyanobacteria, which are engineered to synthesize valuable products directly from CO(2) and sunlight. For example, strains of the model organism Synechocystis sp. PCC 6803 have been ge...

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Autores principales: Böhm, Julien, Kauss, Karsten, Michl, Klaudia, Engelhardt, Lisa, Brouwer, Eva-Maria, Hagemann, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10465007/
https://www.ncbi.nlm.nih.gov/pubmed/37649635
http://dx.doi.org/10.3389/fmicb.2023.1238737
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author Böhm, Julien
Kauss, Karsten
Michl, Klaudia
Engelhardt, Lisa
Brouwer, Eva-Maria
Hagemann, Martin
author_facet Böhm, Julien
Kauss, Karsten
Michl, Klaudia
Engelhardt, Lisa
Brouwer, Eva-Maria
Hagemann, Martin
author_sort Böhm, Julien
collection PubMed
description Future sustainable energy production can be achieved using mass cultures of photoautotrophic microorganisms such as cyanobacteria, which are engineered to synthesize valuable products directly from CO(2) and sunlight. For example, strains of the model organism Synechocystis sp. PCC 6803 have been generated to produce ethanol. Here, we performed a study to prove the hypothesis that carbon flux in the direction of pyruvate is one bottleneck to achieve high ethanol titers in cyanobacteria. Ethanol-producing strains of the cyanobacterium Synechocystis sp. PCC 6803 were generated that bear mutation in the gene pirC aiming to increase carbon flux towards pyruvate. The strains were cultivated at different nitrogen or carbon conditions and the ethanol production was analysed. Generally, a clear correlation between growth rate and ethanol production was found. The mutation of pirC, however, had only a positive impact on ethanol titers under nitrogen depletion. The increase in ethanol was accompanied by elevated pyruvate and lowered glycogen levels indicating that the absence of pirC indeed increased carbon partitioning towards lower glycolysis. Metabolome analysis revealed that this change in carbon flow had also a marked impact on the overall primary metabolism in Synechocystis sp. PCC 6803. Deletion of pirC improved ethanol production under specific conditions supporting the notion that a better understanding of regulatory mechanisms involved in cyanobacterial carbon partitioning is needed to engineer more productive cyanobacterial strains.
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spelling pubmed-104650072023-08-30 Impact of the carbon flux regulator protein pirC on ethanol production in engineered cyanobacteria Böhm, Julien Kauss, Karsten Michl, Klaudia Engelhardt, Lisa Brouwer, Eva-Maria Hagemann, Martin Front Microbiol Microbiology Future sustainable energy production can be achieved using mass cultures of photoautotrophic microorganisms such as cyanobacteria, which are engineered to synthesize valuable products directly from CO(2) and sunlight. For example, strains of the model organism Synechocystis sp. PCC 6803 have been generated to produce ethanol. Here, we performed a study to prove the hypothesis that carbon flux in the direction of pyruvate is one bottleneck to achieve high ethanol titers in cyanobacteria. Ethanol-producing strains of the cyanobacterium Synechocystis sp. PCC 6803 were generated that bear mutation in the gene pirC aiming to increase carbon flux towards pyruvate. The strains were cultivated at different nitrogen or carbon conditions and the ethanol production was analysed. Generally, a clear correlation between growth rate and ethanol production was found. The mutation of pirC, however, had only a positive impact on ethanol titers under nitrogen depletion. The increase in ethanol was accompanied by elevated pyruvate and lowered glycogen levels indicating that the absence of pirC indeed increased carbon partitioning towards lower glycolysis. Metabolome analysis revealed that this change in carbon flow had also a marked impact on the overall primary metabolism in Synechocystis sp. PCC 6803. Deletion of pirC improved ethanol production under specific conditions supporting the notion that a better understanding of regulatory mechanisms involved in cyanobacterial carbon partitioning is needed to engineer more productive cyanobacterial strains. Frontiers Media S.A. 2023-08-15 /pmc/articles/PMC10465007/ /pubmed/37649635 http://dx.doi.org/10.3389/fmicb.2023.1238737 Text en Copyright © 2023 Böhm, Kauss, Michl, Engelhardt, Brouwer and Hagemann. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Böhm, Julien
Kauss, Karsten
Michl, Klaudia
Engelhardt, Lisa
Brouwer, Eva-Maria
Hagemann, Martin
Impact of the carbon flux regulator protein pirC on ethanol production in engineered cyanobacteria
title Impact of the carbon flux regulator protein pirC on ethanol production in engineered cyanobacteria
title_full Impact of the carbon flux regulator protein pirC on ethanol production in engineered cyanobacteria
title_fullStr Impact of the carbon flux regulator protein pirC on ethanol production in engineered cyanobacteria
title_full_unstemmed Impact of the carbon flux regulator protein pirC on ethanol production in engineered cyanobacteria
title_short Impact of the carbon flux regulator protein pirC on ethanol production in engineered cyanobacteria
title_sort impact of the carbon flux regulator protein pirc on ethanol production in engineered cyanobacteria
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10465007/
https://www.ncbi.nlm.nih.gov/pubmed/37649635
http://dx.doi.org/10.3389/fmicb.2023.1238737
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