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Mycelium-based biomaterials as smart devices for skin wound healing
Introduction: Recently, mycelia of Ganoderma lucidum and Pleurotus ostreatus, edible fungi, have been characterized in vitro as self-growing biomaterials for tissue engineering since they are constituted of interconnected fibrous networks resembling the dermal collagen structure. Aim: This work aims...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10465301/ https://www.ncbi.nlm.nih.gov/pubmed/37650039 http://dx.doi.org/10.3389/fbioe.2023.1225722 |
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author | Ruggeri, Marco Miele, Dalila Contardi, Marco Vigani, Barbara Boselli, Cinzia Icaro Cornaglia, Antonia Rossi, Silvia Suarato, Giulia Athanassiou, Athanassia Sandri, Giuseppina |
author_facet | Ruggeri, Marco Miele, Dalila Contardi, Marco Vigani, Barbara Boselli, Cinzia Icaro Cornaglia, Antonia Rossi, Silvia Suarato, Giulia Athanassiou, Athanassia Sandri, Giuseppina |
author_sort | Ruggeri, Marco |
collection | PubMed |
description | Introduction: Recently, mycelia of Ganoderma lucidum and Pleurotus ostreatus, edible fungi, have been characterized in vitro as self-growing biomaterials for tissue engineering since they are constituted of interconnected fibrous networks resembling the dermal collagen structure. Aim: This work aims to investigate the biopharmaceutical properties of G. lucidum and P. ostreatus mycelia to prove their safety and effectiveness in tissue engineering as dermal substitutes. Methods: The mycelial materials were characterized using a multidisciplinary approach, including physicochemical properties (morphology, thermal behavior, surface charge, and isoelectric point). Moreover, preclinical properties such as gene expression and in vitro wound healing assay have been evaluated using fibroblasts. Finally, these naturally-grown substrates were applied in vivo using a murine burn/excisional wound model. Conclusions: Both G. lucidum and P. ostreatus mycelia are biocompatible and able to safely and effectively enhance tissue repair in vivo in our preclinical model. |
format | Online Article Text |
id | pubmed-10465301 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-104653012023-08-30 Mycelium-based biomaterials as smart devices for skin wound healing Ruggeri, Marco Miele, Dalila Contardi, Marco Vigani, Barbara Boselli, Cinzia Icaro Cornaglia, Antonia Rossi, Silvia Suarato, Giulia Athanassiou, Athanassia Sandri, Giuseppina Front Bioeng Biotechnol Bioengineering and Biotechnology Introduction: Recently, mycelia of Ganoderma lucidum and Pleurotus ostreatus, edible fungi, have been characterized in vitro as self-growing biomaterials for tissue engineering since they are constituted of interconnected fibrous networks resembling the dermal collagen structure. Aim: This work aims to investigate the biopharmaceutical properties of G. lucidum and P. ostreatus mycelia to prove their safety and effectiveness in tissue engineering as dermal substitutes. Methods: The mycelial materials were characterized using a multidisciplinary approach, including physicochemical properties (morphology, thermal behavior, surface charge, and isoelectric point). Moreover, preclinical properties such as gene expression and in vitro wound healing assay have been evaluated using fibroblasts. Finally, these naturally-grown substrates were applied in vivo using a murine burn/excisional wound model. Conclusions: Both G. lucidum and P. ostreatus mycelia are biocompatible and able to safely and effectively enhance tissue repair in vivo in our preclinical model. Frontiers Media S.A. 2023-08-15 /pmc/articles/PMC10465301/ /pubmed/37650039 http://dx.doi.org/10.3389/fbioe.2023.1225722 Text en Copyright © 2023 Ruggeri, Miele, Contardi, Vigani, Boselli, Icaro Cornaglia, Rossi, Suarato, Athanassiou and Sandri. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Ruggeri, Marco Miele, Dalila Contardi, Marco Vigani, Barbara Boselli, Cinzia Icaro Cornaglia, Antonia Rossi, Silvia Suarato, Giulia Athanassiou, Athanassia Sandri, Giuseppina Mycelium-based biomaterials as smart devices for skin wound healing |
title | Mycelium-based biomaterials as smart devices for skin wound healing |
title_full | Mycelium-based biomaterials as smart devices for skin wound healing |
title_fullStr | Mycelium-based biomaterials as smart devices for skin wound healing |
title_full_unstemmed | Mycelium-based biomaterials as smart devices for skin wound healing |
title_short | Mycelium-based biomaterials as smart devices for skin wound healing |
title_sort | mycelium-based biomaterials as smart devices for skin wound healing |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10465301/ https://www.ncbi.nlm.nih.gov/pubmed/37650039 http://dx.doi.org/10.3389/fbioe.2023.1225722 |
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