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Temporal changes of periodontal tissue pathology in a periodontitis animal model

PURPOSE: This study aimed to characterize the early stages of periodontal disease and determine the optimal period for its evaluation in a mouse model. The association between the duration of ligation and its effect on the dentogingival area in mice was evaluated using micro-computed tomography (CT)...

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Autores principales: Yoon, Hyunpil, Jung, Bo Hyun, Yoo, Ki-Yeon, Lee, Jong-Bin, Um, Heung-Sik, Chang, Beom-Seok, Lee, Jae-Kwan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Academy of Periodontology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10465809/
https://www.ncbi.nlm.nih.gov/pubmed/36468486
http://dx.doi.org/10.5051/jpis.2203420171
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author Yoon, Hyunpil
Jung, Bo Hyun
Yoo, Ki-Yeon
Lee, Jong-Bin
Um, Heung-Sik
Chang, Beom-Seok
Lee, Jae-Kwan
author_facet Yoon, Hyunpil
Jung, Bo Hyun
Yoo, Ki-Yeon
Lee, Jong-Bin
Um, Heung-Sik
Chang, Beom-Seok
Lee, Jae-Kwan
author_sort Yoon, Hyunpil
collection PubMed
description PURPOSE: This study aimed to characterize the early stages of periodontal disease and determine the optimal period for its evaluation in a mouse model. The association between the duration of ligation and its effect on the dentogingival area in mice was evaluated using micro-computed tomography (CT) and histological analysis. METHODS: Ninety mice were allocated to an untreated control group or a ligation group in which periodontitis was induced by a 6-0 silk ligation around the left second maxillary molar. Mice were sacrificed at 1, 2, 3, 4, 5, 8, 11, and 14 days after ligature placement. Alveolar bone destruction was evaluated using micro-CT. Histological analysis was performed to assess the immune-inflammatory processes in the periodontal tissue. RESULTS: No significant difference in alveolar bone loss was found compared to the control group until day 3 after ligature placement, and a gradual increase in alveolar bone loss was observed from 4 to 8 days following ligature placement. No significant between-group differences were observed after 8 days. The histological analysis demonstrated that the inflammatory response was evident from day 4. CONCLUSIONS: Our findings in a mouse model provide experimental evidence that ligature-induced periodontitis models offer a consistent progression of disease with marginal attachment down-growth, inflammatory infiltration, and alveolar bone loss.
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spelling pubmed-104658092023-08-31 Temporal changes of periodontal tissue pathology in a periodontitis animal model Yoon, Hyunpil Jung, Bo Hyun Yoo, Ki-Yeon Lee, Jong-Bin Um, Heung-Sik Chang, Beom-Seok Lee, Jae-Kwan J Periodontal Implant Sci Research Article PURPOSE: This study aimed to characterize the early stages of periodontal disease and determine the optimal period for its evaluation in a mouse model. The association between the duration of ligation and its effect on the dentogingival area in mice was evaluated using micro-computed tomography (CT) and histological analysis. METHODS: Ninety mice were allocated to an untreated control group or a ligation group in which periodontitis was induced by a 6-0 silk ligation around the left second maxillary molar. Mice were sacrificed at 1, 2, 3, 4, 5, 8, 11, and 14 days after ligature placement. Alveolar bone destruction was evaluated using micro-CT. Histological analysis was performed to assess the immune-inflammatory processes in the periodontal tissue. RESULTS: No significant difference in alveolar bone loss was found compared to the control group until day 3 after ligature placement, and a gradual increase in alveolar bone loss was observed from 4 to 8 days following ligature placement. No significant between-group differences were observed after 8 days. The histological analysis demonstrated that the inflammatory response was evident from day 4. CONCLUSIONS: Our findings in a mouse model provide experimental evidence that ligature-induced periodontitis models offer a consistent progression of disease with marginal attachment down-growth, inflammatory infiltration, and alveolar bone loss. Korean Academy of Periodontology 2022-11-23 /pmc/articles/PMC10465809/ /pubmed/36468486 http://dx.doi.org/10.5051/jpis.2203420171 Text en Copyright © 2023. Korean Academy of Periodontology https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/).
spellingShingle Research Article
Yoon, Hyunpil
Jung, Bo Hyun
Yoo, Ki-Yeon
Lee, Jong-Bin
Um, Heung-Sik
Chang, Beom-Seok
Lee, Jae-Kwan
Temporal changes of periodontal tissue pathology in a periodontitis animal model
title Temporal changes of periodontal tissue pathology in a periodontitis animal model
title_full Temporal changes of periodontal tissue pathology in a periodontitis animal model
title_fullStr Temporal changes of periodontal tissue pathology in a periodontitis animal model
title_full_unstemmed Temporal changes of periodontal tissue pathology in a periodontitis animal model
title_short Temporal changes of periodontal tissue pathology in a periodontitis animal model
title_sort temporal changes of periodontal tissue pathology in a periodontitis animal model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10465809/
https://www.ncbi.nlm.nih.gov/pubmed/36468486
http://dx.doi.org/10.5051/jpis.2203420171
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