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Noncanonical Condensation of Nucleic Acid Chains by Hydrophobic Gold Nanocrystals
[Image: see text] Nucleic acid condensates are essential for various biological processes and have numerous applications in nucleic acid nanotechnology, gene therapy, and mRNA vaccines. However, unlike the in vivo condensation that is dependent on motor proteins, the in vitro condensation efficiency...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10466341/ https://www.ncbi.nlm.nih.gov/pubmed/37654586 http://dx.doi.org/10.1021/jacsau.3c00252 |
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author | Li, Yu Zheng, Haoran Lu, Hui Duan, Mulin Li, Cong Li, Mingqiang Li, Jiang Wang, Lihua Li, Qian Chen, Jing Shen, Jianlei |
author_facet | Li, Yu Zheng, Haoran Lu, Hui Duan, Mulin Li, Cong Li, Mingqiang Li, Jiang Wang, Lihua Li, Qian Chen, Jing Shen, Jianlei |
author_sort | Li, Yu |
collection | PubMed |
description | [Image: see text] Nucleic acid condensates are essential for various biological processes and have numerous applications in nucleic acid nanotechnology, gene therapy, and mRNA vaccines. However, unlike the in vivo condensation that is dependent on motor proteins, the in vitro condensation efficiency remains to be improved. Here, we proposed a hydrophobic interaction-driven mechanism for condensing long nucleic acid chains using atomically precise hydrophobic gold nanoclusters (Au NCs). We found that hydrophobic Au NCs could condense long single-stranded DNA or RNA to form composites of spherical nanostructures, which further assembled into bead-shaped suprastructures in the presence of excessive Au NCs. Thus, suprastructures displayed gel-like behaviors, and Au NCs could diffuse freely inside the condensates, which resemble the collective motions of condensin complexes inside chromosomes. The dynamic hydrophobic interactions between Au NCs and bases allow for the reversible release of nucleic acids in the presence of mild triggering agents. Our method represents a significant advancement toward the development of more efficient and versatile nucleic acid condensation techniques. |
format | Online Article Text |
id | pubmed-10466341 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-104663412023-08-31 Noncanonical Condensation of Nucleic Acid Chains by Hydrophobic Gold Nanocrystals Li, Yu Zheng, Haoran Lu, Hui Duan, Mulin Li, Cong Li, Mingqiang Li, Jiang Wang, Lihua Li, Qian Chen, Jing Shen, Jianlei JACS Au [Image: see text] Nucleic acid condensates are essential for various biological processes and have numerous applications in nucleic acid nanotechnology, gene therapy, and mRNA vaccines. However, unlike the in vivo condensation that is dependent on motor proteins, the in vitro condensation efficiency remains to be improved. Here, we proposed a hydrophobic interaction-driven mechanism for condensing long nucleic acid chains using atomically precise hydrophobic gold nanoclusters (Au NCs). We found that hydrophobic Au NCs could condense long single-stranded DNA or RNA to form composites of spherical nanostructures, which further assembled into bead-shaped suprastructures in the presence of excessive Au NCs. Thus, suprastructures displayed gel-like behaviors, and Au NCs could diffuse freely inside the condensates, which resemble the collective motions of condensin complexes inside chromosomes. The dynamic hydrophobic interactions between Au NCs and bases allow for the reversible release of nucleic acids in the presence of mild triggering agents. Our method represents a significant advancement toward the development of more efficient and versatile nucleic acid condensation techniques. American Chemical Society 2023-08-07 /pmc/articles/PMC10466341/ /pubmed/37654586 http://dx.doi.org/10.1021/jacsau.3c00252 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Li, Yu Zheng, Haoran Lu, Hui Duan, Mulin Li, Cong Li, Mingqiang Li, Jiang Wang, Lihua Li, Qian Chen, Jing Shen, Jianlei Noncanonical Condensation of Nucleic Acid Chains by Hydrophobic Gold Nanocrystals |
title | Noncanonical Condensation of Nucleic Acid Chains by
Hydrophobic Gold Nanocrystals |
title_full | Noncanonical Condensation of Nucleic Acid Chains by
Hydrophobic Gold Nanocrystals |
title_fullStr | Noncanonical Condensation of Nucleic Acid Chains by
Hydrophobic Gold Nanocrystals |
title_full_unstemmed | Noncanonical Condensation of Nucleic Acid Chains by
Hydrophobic Gold Nanocrystals |
title_short | Noncanonical Condensation of Nucleic Acid Chains by
Hydrophobic Gold Nanocrystals |
title_sort | noncanonical condensation of nucleic acid chains by
hydrophobic gold nanocrystals |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10466341/ https://www.ncbi.nlm.nih.gov/pubmed/37654586 http://dx.doi.org/10.1021/jacsau.3c00252 |
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