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Genomic and metabonomic methods reveal the probiotic functions of swine-derived Ligilactobacillus salivarius
BACKGROUND: As substitutes for antibiotics, probiotic bacteria protect against digestive infections caused by pathogenic bacteria. Ligilactobacillus salivarius is a species of native lactobacillus found in both humans and animals. Herein, a swine-derived Ligilactobacillus salivarius was isolated and...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10466738/ https://www.ncbi.nlm.nih.gov/pubmed/37648978 http://dx.doi.org/10.1186/s12866-023-02993-9 |
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author | Yang, Jiajun Shang, Peng Zhang, Bo Wang, Jing Du, Zhenyu Wang, Shanfeng Xing, Jun Zhang, Hao |
author_facet | Yang, Jiajun Shang, Peng Zhang, Bo Wang, Jing Du, Zhenyu Wang, Shanfeng Xing, Jun Zhang, Hao |
author_sort | Yang, Jiajun |
collection | PubMed |
description | BACKGROUND: As substitutes for antibiotics, probiotic bacteria protect against digestive infections caused by pathogenic bacteria. Ligilactobacillus salivarius is a species of native lactobacillus found in both humans and animals. Herein, a swine-derived Ligilactobacillus salivarius was isolated and shown to colonize the ileal mucous membrane, thereby promoting nutritional digestion, absorption, and immunity. To evaluate its probiotic role, the entire genome was sequenced, the genetic information was annotated, and the metabolic information was analyzed. RESULTS: The phylogenetic relationship indicated that the bacteria was closer to L. salivarius MT573555.1 and MT585431.1. Functional genes included transporters, membrane proteins, enzymes, heavy metal resistance proteins, and putative proteins; metabolism-related genes were the most abundant. The six types of metabolic pathways secreted by L. salivarius were mainly composed of secretory transmembrane proteins and peptides. The secretory proteins of L. salivarius were digestive enzymes, functional proteins that regulate apoptosis, antibodies, and hormones. Non-targeted metabolomic analysis of L. salivarius metabolites suggested that ceramide, pyrrolidone- 5- carboxylic acid, N2-acetyl-L-ornithine, 2-ethyl-2-hydroxybutyric acid, N-lactoyl-phenylalanine, and 12 others were involved in antioxidation, repair of the cellular membrane, anticonvulsant, hypnosis, and appetite inhibition. Metabolites of clavaminic acid, antibiotic X14889C, and five other types of bacteriocins were identified, namely phenyllactic acid, janthitrem G, 13-demethyl tacrolimus, medinoside E, and tertonasin. The adherence and antioxidation of L. salivarius were also predicted. No virulence genes were found. CONCLUSION: The main probiotic properties of L. salivarius were identified using genomic, metabonomic, and biochemical assays, which are beneficial for porcine feeding. Our results provided deeper insights into the probiotic effects of L. salivarius. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-023-02993-9. |
format | Online Article Text |
id | pubmed-10466738 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-104667382023-08-31 Genomic and metabonomic methods reveal the probiotic functions of swine-derived Ligilactobacillus salivarius Yang, Jiajun Shang, Peng Zhang, Bo Wang, Jing Du, Zhenyu Wang, Shanfeng Xing, Jun Zhang, Hao BMC Microbiol Research BACKGROUND: As substitutes for antibiotics, probiotic bacteria protect against digestive infections caused by pathogenic bacteria. Ligilactobacillus salivarius is a species of native lactobacillus found in both humans and animals. Herein, a swine-derived Ligilactobacillus salivarius was isolated and shown to colonize the ileal mucous membrane, thereby promoting nutritional digestion, absorption, and immunity. To evaluate its probiotic role, the entire genome was sequenced, the genetic information was annotated, and the metabolic information was analyzed. RESULTS: The phylogenetic relationship indicated that the bacteria was closer to L. salivarius MT573555.1 and MT585431.1. Functional genes included transporters, membrane proteins, enzymes, heavy metal resistance proteins, and putative proteins; metabolism-related genes were the most abundant. The six types of metabolic pathways secreted by L. salivarius were mainly composed of secretory transmembrane proteins and peptides. The secretory proteins of L. salivarius were digestive enzymes, functional proteins that regulate apoptosis, antibodies, and hormones. Non-targeted metabolomic analysis of L. salivarius metabolites suggested that ceramide, pyrrolidone- 5- carboxylic acid, N2-acetyl-L-ornithine, 2-ethyl-2-hydroxybutyric acid, N-lactoyl-phenylalanine, and 12 others were involved in antioxidation, repair of the cellular membrane, anticonvulsant, hypnosis, and appetite inhibition. Metabolites of clavaminic acid, antibiotic X14889C, and five other types of bacteriocins were identified, namely phenyllactic acid, janthitrem G, 13-demethyl tacrolimus, medinoside E, and tertonasin. The adherence and antioxidation of L. salivarius were also predicted. No virulence genes were found. CONCLUSION: The main probiotic properties of L. salivarius were identified using genomic, metabonomic, and biochemical assays, which are beneficial for porcine feeding. Our results provided deeper insights into the probiotic effects of L. salivarius. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-023-02993-9. BioMed Central 2023-08-30 /pmc/articles/PMC10466738/ /pubmed/37648978 http://dx.doi.org/10.1186/s12866-023-02993-9 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Yang, Jiajun Shang, Peng Zhang, Bo Wang, Jing Du, Zhenyu Wang, Shanfeng Xing, Jun Zhang, Hao Genomic and metabonomic methods reveal the probiotic functions of swine-derived Ligilactobacillus salivarius |
title | Genomic and metabonomic methods reveal the probiotic functions of swine-derived Ligilactobacillus salivarius |
title_full | Genomic and metabonomic methods reveal the probiotic functions of swine-derived Ligilactobacillus salivarius |
title_fullStr | Genomic and metabonomic methods reveal the probiotic functions of swine-derived Ligilactobacillus salivarius |
title_full_unstemmed | Genomic and metabonomic methods reveal the probiotic functions of swine-derived Ligilactobacillus salivarius |
title_short | Genomic and metabonomic methods reveal the probiotic functions of swine-derived Ligilactobacillus salivarius |
title_sort | genomic and metabonomic methods reveal the probiotic functions of swine-derived ligilactobacillus salivarius |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10466738/ https://www.ncbi.nlm.nih.gov/pubmed/37648978 http://dx.doi.org/10.1186/s12866-023-02993-9 |
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