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High-throughput bactericidal assays for monoclonal antibody screening against antimicrobial resistant Neisseria gonorrhoeae

Neisseria gonorrhoeae (gonococcus) is an obligate human pathogen and the etiological agent of the sexually transmitted disease gonorrhea. The rapid rise in gonococcal resistance to all currently available antimicrobials has become a significant public health burden and the need to develop novel ther...

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Autores principales: Stazzoni, Samuele, Troisi, Marco, Abbiento, Valentina, Sala, Claudia, Andreano, Emanuele, Rappuoli, Rino
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10466782/
https://www.ncbi.nlm.nih.gov/pubmed/37655342
http://dx.doi.org/10.3389/fmicb.2023.1243427
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author Stazzoni, Samuele
Troisi, Marco
Abbiento, Valentina
Sala, Claudia
Andreano, Emanuele
Rappuoli, Rino
author_facet Stazzoni, Samuele
Troisi, Marco
Abbiento, Valentina
Sala, Claudia
Andreano, Emanuele
Rappuoli, Rino
author_sort Stazzoni, Samuele
collection PubMed
description Neisseria gonorrhoeae (gonococcus) is an obligate human pathogen and the etiological agent of the sexually transmitted disease gonorrhea. The rapid rise in gonococcal resistance to all currently available antimicrobials has become a significant public health burden and the need to develop novel therapeutic and prophylactic tools is now a global priority. While high-throughput screening methods allowed rapid discovery of extremely potent monoclonal antibodies (mAbs) against viral pathogens, the field of bacteriology suffers from the lack of assays that allow efficient screening of large panels of samples. To address this point, we developed luminescence-based (L-ABA) and resazurin-based (R-ABA) antibody bactericidal assays that measure N. gonorrhoeae metabolic activity as a proxy of bacterial viability. Both L-ABA and R-ABA are applicable on the large scale for the rapid identification of bactericidal antibodies and were validated by conventional methods. Implementation of these approaches will be instrumental to the development of new medications and vaccines against N. gonorrhoeae and other bacterial pathogens to support the fight against antimicrobial resistance.
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spelling pubmed-104667822023-08-31 High-throughput bactericidal assays for monoclonal antibody screening against antimicrobial resistant Neisseria gonorrhoeae Stazzoni, Samuele Troisi, Marco Abbiento, Valentina Sala, Claudia Andreano, Emanuele Rappuoli, Rino Front Microbiol Microbiology Neisseria gonorrhoeae (gonococcus) is an obligate human pathogen and the etiological agent of the sexually transmitted disease gonorrhea. The rapid rise in gonococcal resistance to all currently available antimicrobials has become a significant public health burden and the need to develop novel therapeutic and prophylactic tools is now a global priority. While high-throughput screening methods allowed rapid discovery of extremely potent monoclonal antibodies (mAbs) against viral pathogens, the field of bacteriology suffers from the lack of assays that allow efficient screening of large panels of samples. To address this point, we developed luminescence-based (L-ABA) and resazurin-based (R-ABA) antibody bactericidal assays that measure N. gonorrhoeae metabolic activity as a proxy of bacterial viability. Both L-ABA and R-ABA are applicable on the large scale for the rapid identification of bactericidal antibodies and were validated by conventional methods. Implementation of these approaches will be instrumental to the development of new medications and vaccines against N. gonorrhoeae and other bacterial pathogens to support the fight against antimicrobial resistance. Frontiers Media S.A. 2023-08-16 /pmc/articles/PMC10466782/ /pubmed/37655342 http://dx.doi.org/10.3389/fmicb.2023.1243427 Text en Copyright © 2023 Stazzoni, Troisi, Abbiento, Sala, Andreano and Rappuoli. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Stazzoni, Samuele
Troisi, Marco
Abbiento, Valentina
Sala, Claudia
Andreano, Emanuele
Rappuoli, Rino
High-throughput bactericidal assays for monoclonal antibody screening against antimicrobial resistant Neisseria gonorrhoeae
title High-throughput bactericidal assays for monoclonal antibody screening against antimicrobial resistant Neisseria gonorrhoeae
title_full High-throughput bactericidal assays for monoclonal antibody screening against antimicrobial resistant Neisseria gonorrhoeae
title_fullStr High-throughput bactericidal assays for monoclonal antibody screening against antimicrobial resistant Neisseria gonorrhoeae
title_full_unstemmed High-throughput bactericidal assays for monoclonal antibody screening against antimicrobial resistant Neisseria gonorrhoeae
title_short High-throughput bactericidal assays for monoclonal antibody screening against antimicrobial resistant Neisseria gonorrhoeae
title_sort high-throughput bactericidal assays for monoclonal antibody screening against antimicrobial resistant neisseria gonorrhoeae
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10466782/
https://www.ncbi.nlm.nih.gov/pubmed/37655342
http://dx.doi.org/10.3389/fmicb.2023.1243427
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