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The Impact of Storage Temperature and Time on Ergot Alkaloid Concentrations

Ergot sclerotia produce toxic secondary metabolites, ergot alkaloids, that infect cereal crops and grasses. Ergot alkaloids have two isomeric configurations: the C-8-R-isomer (R-epimer), and the C-8-S-isomer (S-epimer). Ergot contaminated matrices, such as cereal grains or grasses, may be stored for...

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Autores principales: Cherewyk, Jensen E., Grusie-Ogilvie, Taylor J., Parker, Sarah E., Blakley, Barry R., Al-Dissi, Ahmad N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10467117/
https://www.ncbi.nlm.nih.gov/pubmed/37624254
http://dx.doi.org/10.3390/toxins15080497
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author Cherewyk, Jensen E.
Grusie-Ogilvie, Taylor J.
Parker, Sarah E.
Blakley, Barry R.
Al-Dissi, Ahmad N.
author_facet Cherewyk, Jensen E.
Grusie-Ogilvie, Taylor J.
Parker, Sarah E.
Blakley, Barry R.
Al-Dissi, Ahmad N.
author_sort Cherewyk, Jensen E.
collection PubMed
description Ergot sclerotia produce toxic secondary metabolites, ergot alkaloids, that infect cereal crops and grasses. Ergot alkaloids have two isomeric configurations: the C-8-R-isomer (R-epimer), and the C-8-S-isomer (S-epimer). Ergot contaminated matrices, such as cereal grains or grasses, may be stored for extended periods at various temperatures before being analyzed, utilized, or consumed. This study assessed the concentration of six common ergot alkaloids in both configurations found in naturally contaminated wheat over time (one, two, and four months) at different temperatures (room temperature, +4 °C, and −20 °C) using ultra-high-performance liquid chromatography–tandem mass spectrometry. The data indicate that the total ergot concentration within a natural contaminated sample varies over time at room temperature, +4 °C, and −20 °C. The total ergot concentration increased until month two, and decreased at month four, independent of temperature (p < 0.05). The total R-epimer concentration appeared to be less stable over time than the total S-epimer concentration. The changes in the total R and total S-epimer concentrations may have been caused by changes in the ergocristine and ergocristinine concentrations, respectively. Time and temperature should be considered when storing potentially contaminated matrices in a laboratory or practical agriculture situations. Quantification of ergot contaminated matrices should occur prior to their use to ensure the most reliable estimates of the concentration of ergot.
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spelling pubmed-104671172023-08-31 The Impact of Storage Temperature and Time on Ergot Alkaloid Concentrations Cherewyk, Jensen E. Grusie-Ogilvie, Taylor J. Parker, Sarah E. Blakley, Barry R. Al-Dissi, Ahmad N. Toxins (Basel) Article Ergot sclerotia produce toxic secondary metabolites, ergot alkaloids, that infect cereal crops and grasses. Ergot alkaloids have two isomeric configurations: the C-8-R-isomer (R-epimer), and the C-8-S-isomer (S-epimer). Ergot contaminated matrices, such as cereal grains or grasses, may be stored for extended periods at various temperatures before being analyzed, utilized, or consumed. This study assessed the concentration of six common ergot alkaloids in both configurations found in naturally contaminated wheat over time (one, two, and four months) at different temperatures (room temperature, +4 °C, and −20 °C) using ultra-high-performance liquid chromatography–tandem mass spectrometry. The data indicate that the total ergot concentration within a natural contaminated sample varies over time at room temperature, +4 °C, and −20 °C. The total ergot concentration increased until month two, and decreased at month four, independent of temperature (p < 0.05). The total R-epimer concentration appeared to be less stable over time than the total S-epimer concentration. The changes in the total R and total S-epimer concentrations may have been caused by changes in the ergocristine and ergocristinine concentrations, respectively. Time and temperature should be considered when storing potentially contaminated matrices in a laboratory or practical agriculture situations. Quantification of ergot contaminated matrices should occur prior to their use to ensure the most reliable estimates of the concentration of ergot. MDPI 2023-08-05 /pmc/articles/PMC10467117/ /pubmed/37624254 http://dx.doi.org/10.3390/toxins15080497 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cherewyk, Jensen E.
Grusie-Ogilvie, Taylor J.
Parker, Sarah E.
Blakley, Barry R.
Al-Dissi, Ahmad N.
The Impact of Storage Temperature and Time on Ergot Alkaloid Concentrations
title The Impact of Storage Temperature and Time on Ergot Alkaloid Concentrations
title_full The Impact of Storage Temperature and Time on Ergot Alkaloid Concentrations
title_fullStr The Impact of Storage Temperature and Time on Ergot Alkaloid Concentrations
title_full_unstemmed The Impact of Storage Temperature and Time on Ergot Alkaloid Concentrations
title_short The Impact of Storage Temperature and Time on Ergot Alkaloid Concentrations
title_sort impact of storage temperature and time on ergot alkaloid concentrations
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10467117/
https://www.ncbi.nlm.nih.gov/pubmed/37624254
http://dx.doi.org/10.3390/toxins15080497
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