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Targeted panel sequencing in the routine diagnosis of mature T- and NK-cell lymphomas: report of 128 cases from two German reference centers
Diagnosing any of the more than 30 types of T-cell lymphomas is considered a challenging task for many pathologists and currently requires morphological expertise as well as the integration of clinical data, immunophenotype, flow cytometry and clonality analyses. Even considering all available infor...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10468607/ https://www.ncbi.nlm.nih.gov/pubmed/37664054 http://dx.doi.org/10.3389/fonc.2023.1231601 |
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author | Böck, Julia Maurus, Katja Gerhard-Hartmann, Elena Brändlein, Stephanie Kurz, Katrin S. Ott, German Anagnostopoulos, Ioannis Rosenwald, Andreas Zamò, Alberto |
author_facet | Böck, Julia Maurus, Katja Gerhard-Hartmann, Elena Brändlein, Stephanie Kurz, Katrin S. Ott, German Anagnostopoulos, Ioannis Rosenwald, Andreas Zamò, Alberto |
author_sort | Böck, Julia |
collection | PubMed |
description | Diagnosing any of the more than 30 types of T-cell lymphomas is considered a challenging task for many pathologists and currently requires morphological expertise as well as the integration of clinical data, immunophenotype, flow cytometry and clonality analyses. Even considering all available information, some margin of doubt might remain using the current diagnostic procedures. In recent times, the genetic landscape of most T-cell lymphomas has been elucidated, showing a number of diagnostically relevant mutations. In addition, recent data indicate that some of these genetic alterations might bear prognostic and predictive value. Extensive genetic analyses, such as whole exome or large panel sequencing are still expensive and time consuming, therefore limiting their application in routine diagnostic. We therefore devoted our effort to develop a lean approach for genetic analysis of T-cell lymphomas, focusing on maximum efficiency rather than exhaustively covering all possible targets. Here we report the results generated with our small amplicon-based panel that could be used routinely on paraffin-embedded and even decalcified samples, on a single sample basis in parallel with other NGS-panels used in our routine diagnostic lab, in a relatively short time and with limited costs. We tested 128 available samples from two German reference centers as part of our routine work up (among which 116 T-cell lymphomas), which is the largest routine diagnostic series reported to date. Our results showed that this assay had a very high rate of technical success (97%) and could detect mutations in the majority (79%) of tested T-cell lymphoma samples. |
format | Online Article Text |
id | pubmed-10468607 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-104686072023-09-01 Targeted panel sequencing in the routine diagnosis of mature T- and NK-cell lymphomas: report of 128 cases from two German reference centers Böck, Julia Maurus, Katja Gerhard-Hartmann, Elena Brändlein, Stephanie Kurz, Katrin S. Ott, German Anagnostopoulos, Ioannis Rosenwald, Andreas Zamò, Alberto Front Oncol Oncology Diagnosing any of the more than 30 types of T-cell lymphomas is considered a challenging task for many pathologists and currently requires morphological expertise as well as the integration of clinical data, immunophenotype, flow cytometry and clonality analyses. Even considering all available information, some margin of doubt might remain using the current diagnostic procedures. In recent times, the genetic landscape of most T-cell lymphomas has been elucidated, showing a number of diagnostically relevant mutations. In addition, recent data indicate that some of these genetic alterations might bear prognostic and predictive value. Extensive genetic analyses, such as whole exome or large panel sequencing are still expensive and time consuming, therefore limiting their application in routine diagnostic. We therefore devoted our effort to develop a lean approach for genetic analysis of T-cell lymphomas, focusing on maximum efficiency rather than exhaustively covering all possible targets. Here we report the results generated with our small amplicon-based panel that could be used routinely on paraffin-embedded and even decalcified samples, on a single sample basis in parallel with other NGS-panels used in our routine diagnostic lab, in a relatively short time and with limited costs. We tested 128 available samples from two German reference centers as part of our routine work up (among which 116 T-cell lymphomas), which is the largest routine diagnostic series reported to date. Our results showed that this assay had a very high rate of technical success (97%) and could detect mutations in the majority (79%) of tested T-cell lymphoma samples. Frontiers Media S.A. 2023-08-16 /pmc/articles/PMC10468607/ /pubmed/37664054 http://dx.doi.org/10.3389/fonc.2023.1231601 Text en Copyright © 2023 Böck, Maurus, Gerhard-Hartmann, Brändlein, Kurz, Ott, Anagnostopoulos, Rosenwald and Zamò https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Oncology Böck, Julia Maurus, Katja Gerhard-Hartmann, Elena Brändlein, Stephanie Kurz, Katrin S. Ott, German Anagnostopoulos, Ioannis Rosenwald, Andreas Zamò, Alberto Targeted panel sequencing in the routine diagnosis of mature T- and NK-cell lymphomas: report of 128 cases from two German reference centers |
title | Targeted panel sequencing in the routine diagnosis of mature T- and NK-cell lymphomas: report of 128 cases from two German reference centers |
title_full | Targeted panel sequencing in the routine diagnosis of mature T- and NK-cell lymphomas: report of 128 cases from two German reference centers |
title_fullStr | Targeted panel sequencing in the routine diagnosis of mature T- and NK-cell lymphomas: report of 128 cases from two German reference centers |
title_full_unstemmed | Targeted panel sequencing in the routine diagnosis of mature T- and NK-cell lymphomas: report of 128 cases from two German reference centers |
title_short | Targeted panel sequencing in the routine diagnosis of mature T- and NK-cell lymphomas: report of 128 cases from two German reference centers |
title_sort | targeted panel sequencing in the routine diagnosis of mature t- and nk-cell lymphomas: report of 128 cases from two german reference centers |
topic | Oncology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10468607/ https://www.ncbi.nlm.nih.gov/pubmed/37664054 http://dx.doi.org/10.3389/fonc.2023.1231601 |
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