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Dietary fat differentially modulates the response of bone marrow-derived macrophages to TLR4 and NOD2 agonists
Consumption of diets high in fat has been linked to the development of obesity and related metabolic complications. Such associations originate from the enhanced, chronic, low-grade inflammation mediated by macrophages in response to translocated bacteria, bacterial products, or dietary constituents...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10468623/ https://www.ncbi.nlm.nih.gov/pubmed/37545346 http://dx.doi.org/10.1177/17534259231193926 |
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author | Shehat, Michael G. Miller, Madelyn H. Calder, Ashley N. Gilbertson, Timothy A. Tigno-Aranjuez, Justine T. |
author_facet | Shehat, Michael G. Miller, Madelyn H. Calder, Ashley N. Gilbertson, Timothy A. Tigno-Aranjuez, Justine T. |
author_sort | Shehat, Michael G. |
collection | PubMed |
description | Consumption of diets high in fat has been linked to the development of obesity and related metabolic complications. Such associations originate from the enhanced, chronic, low-grade inflammation mediated by macrophages in response to translocated bacteria, bacterial products, or dietary constituents such as fatty acids (FAs). Nucleotide-binding Oligomerization Domain 2 (NOD2) senses muramyl dipeptide (MDP), a component of bacterial peptidoglycan. The inability to sense peptidoglycan through NOD2 has been demonstrated to lead to dysbiosis, increased bacterial translocation, inflammation and metabolic dysfunction. Currently, it is unknown how consumption of HFDs with different FA compositions might influence NOD2-dependent responses. In this study, we subjected WT mice to a control diet or to HFDs comprised of various ratios of unsaturated to saturated fats and determined the macrophage response to TLR4 and NOD2 agonists. A HFD with equal ratios of saturated and unsaturated fats enhanced subsequent responsiveness of macrophages to LPS but not to MDP. However, a high-unsaturated fat diet (HUFD) or a high-saturated fat diet (HSFD) both decreased the responsiveness to NOD2 agonists compared to that observed in control diet (CD) fed mice. These data suggest that dietary fatty acid composition can influence the subsequent macrophage responsiveness to bacterial products. |
format | Online Article Text |
id | pubmed-10468623 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-104686232023-09-01 Dietary fat differentially modulates the response of bone marrow-derived macrophages to TLR4 and NOD2 agonists Shehat, Michael G. Miller, Madelyn H. Calder, Ashley N. Gilbertson, Timothy A. Tigno-Aranjuez, Justine T. Innate Immun Original Article Consumption of diets high in fat has been linked to the development of obesity and related metabolic complications. Such associations originate from the enhanced, chronic, low-grade inflammation mediated by macrophages in response to translocated bacteria, bacterial products, or dietary constituents such as fatty acids (FAs). Nucleotide-binding Oligomerization Domain 2 (NOD2) senses muramyl dipeptide (MDP), a component of bacterial peptidoglycan. The inability to sense peptidoglycan through NOD2 has been demonstrated to lead to dysbiosis, increased bacterial translocation, inflammation and metabolic dysfunction. Currently, it is unknown how consumption of HFDs with different FA compositions might influence NOD2-dependent responses. In this study, we subjected WT mice to a control diet or to HFDs comprised of various ratios of unsaturated to saturated fats and determined the macrophage response to TLR4 and NOD2 agonists. A HFD with equal ratios of saturated and unsaturated fats enhanced subsequent responsiveness of macrophages to LPS but not to MDP. However, a high-unsaturated fat diet (HUFD) or a high-saturated fat diet (HSFD) both decreased the responsiveness to NOD2 agonists compared to that observed in control diet (CD) fed mice. These data suggest that dietary fatty acid composition can influence the subsequent macrophage responsiveness to bacterial products. SAGE Publications 2023-08-07 2023-08 /pmc/articles/PMC10468623/ /pubmed/37545346 http://dx.doi.org/10.1177/17534259231193926 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Article Shehat, Michael G. Miller, Madelyn H. Calder, Ashley N. Gilbertson, Timothy A. Tigno-Aranjuez, Justine T. Dietary fat differentially modulates the response of bone marrow-derived macrophages to TLR4 and NOD2 agonists |
title | Dietary fat differentially modulates the response of bone marrow-derived macrophages to TLR4 and NOD2 agonists |
title_full | Dietary fat differentially modulates the response of bone marrow-derived macrophages to TLR4 and NOD2 agonists |
title_fullStr | Dietary fat differentially modulates the response of bone marrow-derived macrophages to TLR4 and NOD2 agonists |
title_full_unstemmed | Dietary fat differentially modulates the response of bone marrow-derived macrophages to TLR4 and NOD2 agonists |
title_short | Dietary fat differentially modulates the response of bone marrow-derived macrophages to TLR4 and NOD2 agonists |
title_sort | dietary fat differentially modulates the response of bone marrow-derived macrophages to tlr4 and nod2 agonists |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10468623/ https://www.ncbi.nlm.nih.gov/pubmed/37545346 http://dx.doi.org/10.1177/17534259231193926 |
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