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Synthesis and Characterization of Photo-Cross-Linkable Silk Fibroin Methacryloyl Hydrogel for Biomedical Applications

[Image: see text] Photo-cross-linkable hydrogels have recently gained increased interest in the field of biomedical applications. In this study, silk fibroin was derivatized with methacrylic anhydride (MA) to obtain silk fibroin methacryloyl (SFMA), forming hydrogel under UV light exposure in 1 min....

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Autores principales: Wu, Jianhua, Lei, Jiang, Chen, Ming, Sun, Yusheng, Jianwen, Hou, Li, Suanao, Gang, Liu, Zhang, Mingyang, Yixin, Shen, Zhang, Feng, Zhengshi, Zhang, Fan, Zhihai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10468767/
https://www.ncbi.nlm.nih.gov/pubmed/37663496
http://dx.doi.org/10.1021/acsomega.3c01483
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author Wu, Jianhua
Lei, Jiang
Chen, Ming
Sun, Yusheng
Jianwen, Hou
Li, Suanao
Gang, Liu
Zhang, Mingyang
Yixin, Shen
Zhang, Feng
Zhengshi, Zhang
Fan, Zhihai
author_facet Wu, Jianhua
Lei, Jiang
Chen, Ming
Sun, Yusheng
Jianwen, Hou
Li, Suanao
Gang, Liu
Zhang, Mingyang
Yixin, Shen
Zhang, Feng
Zhengshi, Zhang
Fan, Zhihai
author_sort Wu, Jianhua
collection PubMed
description [Image: see text] Photo-cross-linkable hydrogels have recently gained increased interest in the field of biomedical applications. In this study, silk fibroin was derivatized with methacrylic anhydride (MA) to obtain silk fibroin methacryloyl (SFMA), forming hydrogel under UV light exposure in 1 min. The SFMA sol–gel transition did not involve significant structural change at the early stage. Then, the formation of the irreversible β-sheet was confirmed after 24 h. The resulting SFMA hydrogel showed a homogeneous porous structure with pore sizes ranging from 400 to 700 μm, depending on the content. In addition, these hydrogels demonstrated a lower swelling capacity, higher rheological properties and compressive modulus, and slow degradation behavior at higher content, likely due to the higher degree of cross-linking. An experiment with cells indicated the good cell compatibility of these hydrogels, as revealed by Cell Counting Kit-8 (CCK-8) assays. As a tissue-engineered material, this photo-cross-linkable SFMA is expected to have a wide range of applications in the biomedical field.
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spelling pubmed-104687672023-09-01 Synthesis and Characterization of Photo-Cross-Linkable Silk Fibroin Methacryloyl Hydrogel for Biomedical Applications Wu, Jianhua Lei, Jiang Chen, Ming Sun, Yusheng Jianwen, Hou Li, Suanao Gang, Liu Zhang, Mingyang Yixin, Shen Zhang, Feng Zhengshi, Zhang Fan, Zhihai ACS Omega [Image: see text] Photo-cross-linkable hydrogels have recently gained increased interest in the field of biomedical applications. In this study, silk fibroin was derivatized with methacrylic anhydride (MA) to obtain silk fibroin methacryloyl (SFMA), forming hydrogel under UV light exposure in 1 min. The SFMA sol–gel transition did not involve significant structural change at the early stage. Then, the formation of the irreversible β-sheet was confirmed after 24 h. The resulting SFMA hydrogel showed a homogeneous porous structure with pore sizes ranging from 400 to 700 μm, depending on the content. In addition, these hydrogels demonstrated a lower swelling capacity, higher rheological properties and compressive modulus, and slow degradation behavior at higher content, likely due to the higher degree of cross-linking. An experiment with cells indicated the good cell compatibility of these hydrogels, as revealed by Cell Counting Kit-8 (CCK-8) assays. As a tissue-engineered material, this photo-cross-linkable SFMA is expected to have a wide range of applications in the biomedical field. American Chemical Society 2023-08-15 /pmc/articles/PMC10468767/ /pubmed/37663496 http://dx.doi.org/10.1021/acsomega.3c01483 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Wu, Jianhua
Lei, Jiang
Chen, Ming
Sun, Yusheng
Jianwen, Hou
Li, Suanao
Gang, Liu
Zhang, Mingyang
Yixin, Shen
Zhang, Feng
Zhengshi, Zhang
Fan, Zhihai
Synthesis and Characterization of Photo-Cross-Linkable Silk Fibroin Methacryloyl Hydrogel for Biomedical Applications
title Synthesis and Characterization of Photo-Cross-Linkable Silk Fibroin Methacryloyl Hydrogel for Biomedical Applications
title_full Synthesis and Characterization of Photo-Cross-Linkable Silk Fibroin Methacryloyl Hydrogel for Biomedical Applications
title_fullStr Synthesis and Characterization of Photo-Cross-Linkable Silk Fibroin Methacryloyl Hydrogel for Biomedical Applications
title_full_unstemmed Synthesis and Characterization of Photo-Cross-Linkable Silk Fibroin Methacryloyl Hydrogel for Biomedical Applications
title_short Synthesis and Characterization of Photo-Cross-Linkable Silk Fibroin Methacryloyl Hydrogel for Biomedical Applications
title_sort synthesis and characterization of photo-cross-linkable silk fibroin methacryloyl hydrogel for biomedical applications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10468767/
https://www.ncbi.nlm.nih.gov/pubmed/37663496
http://dx.doi.org/10.1021/acsomega.3c01483
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