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Salting-Out-Assisted Liquid–Liquid Extraction Method for the Determination of Nicotine from Oral Traditional and Innovative Tobacco Products Using UPLC-MS/MS
[Image: see text] In this study, we developed and validated a novel method that allows for the extraction and quantitation of nicotine from a variety of commercially available oral tobacco products including loose and pouched traditional moist smokeless tobacco products, and oral tobacco-derived nic...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10468829/ https://www.ncbi.nlm.nih.gov/pubmed/37663509 http://dx.doi.org/10.1021/acsomega.3c03474 |
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author | Aldeek, Fadi Lopez, Vanessa Miller, John H. |
author_facet | Aldeek, Fadi Lopez, Vanessa Miller, John H. |
author_sort | Aldeek, Fadi |
collection | PubMed |
description | [Image: see text] In this study, we developed and validated a novel method that allows for the extraction and quantitation of nicotine from a variety of commercially available oral tobacco products including loose and pouched traditional moist smokeless tobacco products, and oral tobacco-derived nicotine (OTDN) lozenges, gums, and pouches. The method employed an extraction technique consisting of salting-out assisted liquid–liquid extraction using sodium hydroxide and acetonitrile in conjunction with ultra-high pressure liquid chromatography coupled to mass spectrometry. Accurate quantitation was obtained using nicotine methyl-d(3) isotopically labeled internal standard. Chromatographic separation of nicotine and nicotine methyl-d(3) internal standard was achieved using a Waters Acquity C18 column (50 mm × 2.1 mm i.d., 2.5 μm) with 10 mM ammonium acetate buffer (pH = 10) and acetonitrile as mobile phase A and B, respectively. Using a gradient elution and a flow rate of 0.4 mL/min for 5 min runtime, nicotine eluted at 1.74 min. The method was validated according to ICH guidelines for all the sample types with an accuracy for nicotine within 89–109%. Repeatability and intermediate precision were both estimated to be ≤7% relative standard deviation (% RSD). This method is applicable for a wide range of traditional moist smokeless and OTDN tobacco products. |
format | Online Article Text |
id | pubmed-10468829 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-104688292023-09-01 Salting-Out-Assisted Liquid–Liquid Extraction Method for the Determination of Nicotine from Oral Traditional and Innovative Tobacco Products Using UPLC-MS/MS Aldeek, Fadi Lopez, Vanessa Miller, John H. ACS Omega [Image: see text] In this study, we developed and validated a novel method that allows for the extraction and quantitation of nicotine from a variety of commercially available oral tobacco products including loose and pouched traditional moist smokeless tobacco products, and oral tobacco-derived nicotine (OTDN) lozenges, gums, and pouches. The method employed an extraction technique consisting of salting-out assisted liquid–liquid extraction using sodium hydroxide and acetonitrile in conjunction with ultra-high pressure liquid chromatography coupled to mass spectrometry. Accurate quantitation was obtained using nicotine methyl-d(3) isotopically labeled internal standard. Chromatographic separation of nicotine and nicotine methyl-d(3) internal standard was achieved using a Waters Acquity C18 column (50 mm × 2.1 mm i.d., 2.5 μm) with 10 mM ammonium acetate buffer (pH = 10) and acetonitrile as mobile phase A and B, respectively. Using a gradient elution and a flow rate of 0.4 mL/min for 5 min runtime, nicotine eluted at 1.74 min. The method was validated according to ICH guidelines for all the sample types with an accuracy for nicotine within 89–109%. Repeatability and intermediate precision were both estimated to be ≤7% relative standard deviation (% RSD). This method is applicable for a wide range of traditional moist smokeless and OTDN tobacco products. American Chemical Society 2023-08-16 /pmc/articles/PMC10468829/ /pubmed/37663509 http://dx.doi.org/10.1021/acsomega.3c03474 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Aldeek, Fadi Lopez, Vanessa Miller, John H. Salting-Out-Assisted Liquid–Liquid Extraction Method for the Determination of Nicotine from Oral Traditional and Innovative Tobacco Products Using UPLC-MS/MS |
title | Salting-Out-Assisted
Liquid–Liquid Extraction
Method for the Determination of Nicotine from Oral Traditional and
Innovative Tobacco Products Using UPLC-MS/MS |
title_full | Salting-Out-Assisted
Liquid–Liquid Extraction
Method for the Determination of Nicotine from Oral Traditional and
Innovative Tobacco Products Using UPLC-MS/MS |
title_fullStr | Salting-Out-Assisted
Liquid–Liquid Extraction
Method for the Determination of Nicotine from Oral Traditional and
Innovative Tobacco Products Using UPLC-MS/MS |
title_full_unstemmed | Salting-Out-Assisted
Liquid–Liquid Extraction
Method for the Determination of Nicotine from Oral Traditional and
Innovative Tobacco Products Using UPLC-MS/MS |
title_short | Salting-Out-Assisted
Liquid–Liquid Extraction
Method for the Determination of Nicotine from Oral Traditional and
Innovative Tobacco Products Using UPLC-MS/MS |
title_sort | salting-out-assisted
liquid–liquid extraction
method for the determination of nicotine from oral traditional and
innovative tobacco products using uplc-ms/ms |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10468829/ https://www.ncbi.nlm.nih.gov/pubmed/37663509 http://dx.doi.org/10.1021/acsomega.3c03474 |
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