Cargando…

A comparative metabolomics study between grain-sized moxibustion and suspended moxibustion on rats with gastric ulcers

Grain-sized moxibustion (GS-Moxi) and suspended moxibustion (S-Moxi) represent the two typical local heat therapies in Traditional Chinese Medicine (TCM) and have been extensively used in treating gastric ulcers (GU) in China. However, the difference in biological response between the two moxibustio...

Descripción completa

Detalles Bibliográficos
Autores principales: Wei, Guhang, Xie, Yufeng, Pei, Mengran, Yang, Jinlan, Yu, Yunjin, Cheng, Yanbin, Chen, Baohua, Guo, Jingjing, Yang, Zongbao, Feng, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10469062/
https://www.ncbi.nlm.nih.gov/pubmed/37664739
http://dx.doi.org/10.1016/j.heliyon.2023.e19108
_version_ 1785099362229026816
author Wei, Guhang
Xie, Yufeng
Pei, Mengran
Yang, Jinlan
Yu, Yunjin
Cheng, Yanbin
Chen, Baohua
Guo, Jingjing
Yang, Zongbao
Feng, Jun
author_facet Wei, Guhang
Xie, Yufeng
Pei, Mengran
Yang, Jinlan
Yu, Yunjin
Cheng, Yanbin
Chen, Baohua
Guo, Jingjing
Yang, Zongbao
Feng, Jun
author_sort Wei, Guhang
collection PubMed
description Grain-sized moxibustion (GS-Moxi) and suspended moxibustion (S-Moxi) represent the two typical local heat therapies in Traditional Chinese Medicine (TCM) and have been extensively used in treating gastric ulcers (GU) in China. However, the difference in biological response between the two moxibustion therapies in treating GU remains unclear. Here we investigated the therapeutic effect and potential mechanistic difference underlying the two moxibustion methods. Ethanol-induced GU model was established and was treated with GS-Moxi or S-Moxi at ST36 and ST21 for 5 days separately. And then, gastric histopathological examination, immunohistochemical staining for repair factors (EGFR, VEGF, Ki67), and (1)H NMR-based metabolomics analysis of plasma and stomach of rats were conducted. We found GS-Moxi and S-Moxi effectively alleviated gastric damage and significantly increased the expression of related repair factors. However, S-Moxi corrected aberrant energy metabolism and lipids metabolism in GU rats but had little effect on neurotransmitter-related metabolism, while GS-Moxi regulated energy metabolism and neurotransmitter-related metabolism in GU rats but had no effect on lipids metabolism. We further proposed that the main target of S-Moxi may be liver and vasculature, whereas GS-Moxi specially targeted the stomach via regulating nervous system. This study strongly verified the outstanding gastroprotective effects of moxibustion and enriched our understanding of the varied biological responses triggered by different moxibustion methods.
format Online
Article
Text
id pubmed-10469062
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-104690622023-09-01 A comparative metabolomics study between grain-sized moxibustion and suspended moxibustion on rats with gastric ulcers Wei, Guhang Xie, Yufeng Pei, Mengran Yang, Jinlan Yu, Yunjin Cheng, Yanbin Chen, Baohua Guo, Jingjing Yang, Zongbao Feng, Jun Heliyon Research Article Grain-sized moxibustion (GS-Moxi) and suspended moxibustion (S-Moxi) represent the two typical local heat therapies in Traditional Chinese Medicine (TCM) and have been extensively used in treating gastric ulcers (GU) in China. However, the difference in biological response between the two moxibustion therapies in treating GU remains unclear. Here we investigated the therapeutic effect and potential mechanistic difference underlying the two moxibustion methods. Ethanol-induced GU model was established and was treated with GS-Moxi or S-Moxi at ST36 and ST21 for 5 days separately. And then, gastric histopathological examination, immunohistochemical staining for repair factors (EGFR, VEGF, Ki67), and (1)H NMR-based metabolomics analysis of plasma and stomach of rats were conducted. We found GS-Moxi and S-Moxi effectively alleviated gastric damage and significantly increased the expression of related repair factors. However, S-Moxi corrected aberrant energy metabolism and lipids metabolism in GU rats but had little effect on neurotransmitter-related metabolism, while GS-Moxi regulated energy metabolism and neurotransmitter-related metabolism in GU rats but had no effect on lipids metabolism. We further proposed that the main target of S-Moxi may be liver and vasculature, whereas GS-Moxi specially targeted the stomach via regulating nervous system. This study strongly verified the outstanding gastroprotective effects of moxibustion and enriched our understanding of the varied biological responses triggered by different moxibustion methods. Elsevier 2023-08-11 /pmc/articles/PMC10469062/ /pubmed/37664739 http://dx.doi.org/10.1016/j.heliyon.2023.e19108 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Wei, Guhang
Xie, Yufeng
Pei, Mengran
Yang, Jinlan
Yu, Yunjin
Cheng, Yanbin
Chen, Baohua
Guo, Jingjing
Yang, Zongbao
Feng, Jun
A comparative metabolomics study between grain-sized moxibustion and suspended moxibustion on rats with gastric ulcers
title A comparative metabolomics study between grain-sized moxibustion and suspended moxibustion on rats with gastric ulcers
title_full A comparative metabolomics study between grain-sized moxibustion and suspended moxibustion on rats with gastric ulcers
title_fullStr A comparative metabolomics study between grain-sized moxibustion and suspended moxibustion on rats with gastric ulcers
title_full_unstemmed A comparative metabolomics study between grain-sized moxibustion and suspended moxibustion on rats with gastric ulcers
title_short A comparative metabolomics study between grain-sized moxibustion and suspended moxibustion on rats with gastric ulcers
title_sort comparative metabolomics study between grain-sized moxibustion and suspended moxibustion on rats with gastric ulcers
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10469062/
https://www.ncbi.nlm.nih.gov/pubmed/37664739
http://dx.doi.org/10.1016/j.heliyon.2023.e19108
work_keys_str_mv AT weiguhang acomparativemetabolomicsstudybetweengrainsizedmoxibustionandsuspendedmoxibustiononratswithgastriculcers
AT xieyufeng acomparativemetabolomicsstudybetweengrainsizedmoxibustionandsuspendedmoxibustiononratswithgastriculcers
AT peimengran acomparativemetabolomicsstudybetweengrainsizedmoxibustionandsuspendedmoxibustiononratswithgastriculcers
AT yangjinlan acomparativemetabolomicsstudybetweengrainsizedmoxibustionandsuspendedmoxibustiononratswithgastriculcers
AT yuyunjin acomparativemetabolomicsstudybetweengrainsizedmoxibustionandsuspendedmoxibustiononratswithgastriculcers
AT chengyanbin acomparativemetabolomicsstudybetweengrainsizedmoxibustionandsuspendedmoxibustiononratswithgastriculcers
AT chenbaohua acomparativemetabolomicsstudybetweengrainsizedmoxibustionandsuspendedmoxibustiononratswithgastriculcers
AT guojingjing acomparativemetabolomicsstudybetweengrainsizedmoxibustionandsuspendedmoxibustiononratswithgastriculcers
AT yangzongbao acomparativemetabolomicsstudybetweengrainsizedmoxibustionandsuspendedmoxibustiononratswithgastriculcers
AT fengjun acomparativemetabolomicsstudybetweengrainsizedmoxibustionandsuspendedmoxibustiononratswithgastriculcers
AT weiguhang comparativemetabolomicsstudybetweengrainsizedmoxibustionandsuspendedmoxibustiononratswithgastriculcers
AT xieyufeng comparativemetabolomicsstudybetweengrainsizedmoxibustionandsuspendedmoxibustiononratswithgastriculcers
AT peimengran comparativemetabolomicsstudybetweengrainsizedmoxibustionandsuspendedmoxibustiononratswithgastriculcers
AT yangjinlan comparativemetabolomicsstudybetweengrainsizedmoxibustionandsuspendedmoxibustiononratswithgastriculcers
AT yuyunjin comparativemetabolomicsstudybetweengrainsizedmoxibustionandsuspendedmoxibustiononratswithgastriculcers
AT chengyanbin comparativemetabolomicsstudybetweengrainsizedmoxibustionandsuspendedmoxibustiononratswithgastriculcers
AT chenbaohua comparativemetabolomicsstudybetweengrainsizedmoxibustionandsuspendedmoxibustiononratswithgastriculcers
AT guojingjing comparativemetabolomicsstudybetweengrainsizedmoxibustionandsuspendedmoxibustiononratswithgastriculcers
AT yangzongbao comparativemetabolomicsstudybetweengrainsizedmoxibustionandsuspendedmoxibustiononratswithgastriculcers
AT fengjun comparativemetabolomicsstudybetweengrainsizedmoxibustionandsuspendedmoxibustiononratswithgastriculcers