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A comparative metabolomics study between grain-sized moxibustion and suspended moxibustion on rats with gastric ulcers
Grain-sized moxibustion (GS-Moxi) and suspended moxibustion (S-Moxi) represent the two typical local heat therapies in Traditional Chinese Medicine (TCM) and have been extensively used in treating gastric ulcers (GU) in China. However, the difference in biological response between the two moxibustio...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10469062/ https://www.ncbi.nlm.nih.gov/pubmed/37664739 http://dx.doi.org/10.1016/j.heliyon.2023.e19108 |
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author | Wei, Guhang Xie, Yufeng Pei, Mengran Yang, Jinlan Yu, Yunjin Cheng, Yanbin Chen, Baohua Guo, Jingjing Yang, Zongbao Feng, Jun |
author_facet | Wei, Guhang Xie, Yufeng Pei, Mengran Yang, Jinlan Yu, Yunjin Cheng, Yanbin Chen, Baohua Guo, Jingjing Yang, Zongbao Feng, Jun |
author_sort | Wei, Guhang |
collection | PubMed |
description | Grain-sized moxibustion (GS-Moxi) and suspended moxibustion (S-Moxi) represent the two typical local heat therapies in Traditional Chinese Medicine (TCM) and have been extensively used in treating gastric ulcers (GU) in China. However, the difference in biological response between the two moxibustion therapies in treating GU remains unclear. Here we investigated the therapeutic effect and potential mechanistic difference underlying the two moxibustion methods. Ethanol-induced GU model was established and was treated with GS-Moxi or S-Moxi at ST36 and ST21 for 5 days separately. And then, gastric histopathological examination, immunohistochemical staining for repair factors (EGFR, VEGF, Ki67), and (1)H NMR-based metabolomics analysis of plasma and stomach of rats were conducted. We found GS-Moxi and S-Moxi effectively alleviated gastric damage and significantly increased the expression of related repair factors. However, S-Moxi corrected aberrant energy metabolism and lipids metabolism in GU rats but had little effect on neurotransmitter-related metabolism, while GS-Moxi regulated energy metabolism and neurotransmitter-related metabolism in GU rats but had no effect on lipids metabolism. We further proposed that the main target of S-Moxi may be liver and vasculature, whereas GS-Moxi specially targeted the stomach via regulating nervous system. This study strongly verified the outstanding gastroprotective effects of moxibustion and enriched our understanding of the varied biological responses triggered by different moxibustion methods. |
format | Online Article Text |
id | pubmed-10469062 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-104690622023-09-01 A comparative metabolomics study between grain-sized moxibustion and suspended moxibustion on rats with gastric ulcers Wei, Guhang Xie, Yufeng Pei, Mengran Yang, Jinlan Yu, Yunjin Cheng, Yanbin Chen, Baohua Guo, Jingjing Yang, Zongbao Feng, Jun Heliyon Research Article Grain-sized moxibustion (GS-Moxi) and suspended moxibustion (S-Moxi) represent the two typical local heat therapies in Traditional Chinese Medicine (TCM) and have been extensively used in treating gastric ulcers (GU) in China. However, the difference in biological response between the two moxibustion therapies in treating GU remains unclear. Here we investigated the therapeutic effect and potential mechanistic difference underlying the two moxibustion methods. Ethanol-induced GU model was established and was treated with GS-Moxi or S-Moxi at ST36 and ST21 for 5 days separately. And then, gastric histopathological examination, immunohistochemical staining for repair factors (EGFR, VEGF, Ki67), and (1)H NMR-based metabolomics analysis of plasma and stomach of rats were conducted. We found GS-Moxi and S-Moxi effectively alleviated gastric damage and significantly increased the expression of related repair factors. However, S-Moxi corrected aberrant energy metabolism and lipids metabolism in GU rats but had little effect on neurotransmitter-related metabolism, while GS-Moxi regulated energy metabolism and neurotransmitter-related metabolism in GU rats but had no effect on lipids metabolism. We further proposed that the main target of S-Moxi may be liver and vasculature, whereas GS-Moxi specially targeted the stomach via regulating nervous system. This study strongly verified the outstanding gastroprotective effects of moxibustion and enriched our understanding of the varied biological responses triggered by different moxibustion methods. Elsevier 2023-08-11 /pmc/articles/PMC10469062/ /pubmed/37664739 http://dx.doi.org/10.1016/j.heliyon.2023.e19108 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Wei, Guhang Xie, Yufeng Pei, Mengran Yang, Jinlan Yu, Yunjin Cheng, Yanbin Chen, Baohua Guo, Jingjing Yang, Zongbao Feng, Jun A comparative metabolomics study between grain-sized moxibustion and suspended moxibustion on rats with gastric ulcers |
title | A comparative metabolomics study between grain-sized moxibustion and suspended moxibustion on rats with gastric ulcers |
title_full | A comparative metabolomics study between grain-sized moxibustion and suspended moxibustion on rats with gastric ulcers |
title_fullStr | A comparative metabolomics study between grain-sized moxibustion and suspended moxibustion on rats with gastric ulcers |
title_full_unstemmed | A comparative metabolomics study between grain-sized moxibustion and suspended moxibustion on rats with gastric ulcers |
title_short | A comparative metabolomics study between grain-sized moxibustion and suspended moxibustion on rats with gastric ulcers |
title_sort | comparative metabolomics study between grain-sized moxibustion and suspended moxibustion on rats with gastric ulcers |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10469062/ https://www.ncbi.nlm.nih.gov/pubmed/37664739 http://dx.doi.org/10.1016/j.heliyon.2023.e19108 |
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