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Establishment and evaluation of a multiplex real-time RT-PCR for quantitative and differential detection of wild-type canine distemper virus from vaccine strains

This study sought to establish a real-time reverse transcription (RT)-PCR method to differentially detect canine distemper virus (CDV) wild-type and vaccine strains. To this end, a pair of CDV universal primers and two specific minor groove binder (MGB) probes, harboring a T/C substitution in the he...

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Detalles Bibliográficos
Autores principales: Sui, Ping, Sun, Yiyang, Shi, Yijun, Ran, Wei, Shi, Ning, Sun, Dongbo, Zheng, Jiasan, Zhao, Jianjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10469063/
https://www.ncbi.nlm.nih.gov/pubmed/37662817
http://dx.doi.org/10.1016/j.heliyon.2023.e19344

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