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Establishment and evaluation of a multiplex real-time RT-PCR for quantitative and differential detection of wild-type canine distemper virus from vaccine strains
This study sought to establish a real-time reverse transcription (RT)-PCR method to differentially detect canine distemper virus (CDV) wild-type and vaccine strains. To this end, a pair of CDV universal primers and two specific minor groove binder (MGB) probes, harboring a T/C substitution in the he...
Autores principales: | Sui, Ping, Sun, Yiyang, Shi, Yijun, Ran, Wei, Shi, Ning, Sun, Dongbo, Zheng, Jiasan, Zhao, Jianjun |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10469063/ https://www.ncbi.nlm.nih.gov/pubmed/37662817 http://dx.doi.org/10.1016/j.heliyon.2023.e19344 |
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