Using Taxol-sensitized budding yeast to investigate the effect of microtubule stabilization on anaphase onset

The microtubule (MT)-stabilizing drug Taxol (paclitaxel) is a commonly used tool to investigate MT dynamics and MT-dependent processes. Here, we present a protocol for using Taxol-sensitized budding yeast to investigate the effect of microtubule stabilization on anaphase onset. We describe steps for...

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Detalles Bibliográficos
Autores principales: Bunning, Angela R., Anderson, Samuel J., Gupta, Mohan L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10469069/
https://www.ncbi.nlm.nih.gov/pubmed/37597189
http://dx.doi.org/10.1016/j.xpro.2023.102522
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author Bunning, Angela R.
Anderson, Samuel J.
Gupta, Mohan L.
author_facet Bunning, Angela R.
Anderson, Samuel J.
Gupta, Mohan L.
author_sort Bunning, Angela R.
collection PubMed
description The microtubule (MT)-stabilizing drug Taxol (paclitaxel) is a commonly used tool to investigate MT dynamics and MT-dependent processes. Here, we present a protocol for using Taxol-sensitized budding yeast to investigate the effect of microtubule stabilization on anaphase onset. We describe steps for establishing a log phase culture, synchronizing cells in G1, arresting in metaphase, and releasing cells into Taxol. We then detail procedures for imaging and scoring anaphase onset. This protocol facilitates maintenance and reproducibility in testing drug-sensitized and Taxol-sensitized yeast strains. For complete details on the use and execution of this protocol, please refer to Proudfoot et al.(1)
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spelling pubmed-104690692023-09-01 Using Taxol-sensitized budding yeast to investigate the effect of microtubule stabilization on anaphase onset Bunning, Angela R. Anderson, Samuel J. Gupta, Mohan L. STAR Protoc Protocol The microtubule (MT)-stabilizing drug Taxol (paclitaxel) is a commonly used tool to investigate MT dynamics and MT-dependent processes. Here, we present a protocol for using Taxol-sensitized budding yeast to investigate the effect of microtubule stabilization on anaphase onset. We describe steps for establishing a log phase culture, synchronizing cells in G1, arresting in metaphase, and releasing cells into Taxol. We then detail procedures for imaging and scoring anaphase onset. This protocol facilitates maintenance and reproducibility in testing drug-sensitized and Taxol-sensitized yeast strains. For complete details on the use and execution of this protocol, please refer to Proudfoot et al.(1) Elsevier 2023-08-18 /pmc/articles/PMC10469069/ /pubmed/37597189 http://dx.doi.org/10.1016/j.xpro.2023.102522 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Bunning, Angela R.
Anderson, Samuel J.
Gupta, Mohan L.
Using Taxol-sensitized budding yeast to investigate the effect of microtubule stabilization on anaphase onset
title Using Taxol-sensitized budding yeast to investigate the effect of microtubule stabilization on anaphase onset
title_full Using Taxol-sensitized budding yeast to investigate the effect of microtubule stabilization on anaphase onset
title_fullStr Using Taxol-sensitized budding yeast to investigate the effect of microtubule stabilization on anaphase onset
title_full_unstemmed Using Taxol-sensitized budding yeast to investigate the effect of microtubule stabilization on anaphase onset
title_short Using Taxol-sensitized budding yeast to investigate the effect of microtubule stabilization on anaphase onset
title_sort using taxol-sensitized budding yeast to investigate the effect of microtubule stabilization on anaphase onset
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10469069/
https://www.ncbi.nlm.nih.gov/pubmed/37597189
http://dx.doi.org/10.1016/j.xpro.2023.102522
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