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Polycomb proteins translate histone methylation to chromatin folding

Epigenetic repression often involves covalent histone modifications. Yet, how the presence of a histone mark translates into changes in chromatin structure that ultimately benefits the repression is largely unclear. Polycomb group proteins comprise a family of evolutionarily conserved epigenetic rep...

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Detalles Bibliográficos
Autores principales: Lizana, Ludvig, Nahali, Negar, Schwartz, Yuri B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10470199/
https://www.ncbi.nlm.nih.gov/pubmed/37499944
http://dx.doi.org/10.1016/j.jbc.2023.105080
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author Lizana, Ludvig
Nahali, Negar
Schwartz, Yuri B.
author_facet Lizana, Ludvig
Nahali, Negar
Schwartz, Yuri B.
author_sort Lizana, Ludvig
collection PubMed
description Epigenetic repression often involves covalent histone modifications. Yet, how the presence of a histone mark translates into changes in chromatin structure that ultimately benefits the repression is largely unclear. Polycomb group proteins comprise a family of evolutionarily conserved epigenetic repressors. They act as multi-subunit complexes one of which tri-methylates histone H3 at Lysine 27 (H3K27). Here we describe a novel Monte Carlo–Molecular Dynamics simulation framework, which we employed to discover that stochastic interaction of Polycomb Repressive Complex 1 (PRC1) with tri-methylated H3K27 is sufficient to fold the methylated chromatin. Unexpectedly, such chromatin folding leads to spatial clustering of the DNA elements bound by PRC1. Our results provide further insight into mechanisms of epigenetic repression and the process of chromatin folding in response to histone methylation.
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spelling pubmed-104701992023-09-01 Polycomb proteins translate histone methylation to chromatin folding Lizana, Ludvig Nahali, Negar Schwartz, Yuri B. J Biol Chem Research Article Epigenetic repression often involves covalent histone modifications. Yet, how the presence of a histone mark translates into changes in chromatin structure that ultimately benefits the repression is largely unclear. Polycomb group proteins comprise a family of evolutionarily conserved epigenetic repressors. They act as multi-subunit complexes one of which tri-methylates histone H3 at Lysine 27 (H3K27). Here we describe a novel Monte Carlo–Molecular Dynamics simulation framework, which we employed to discover that stochastic interaction of Polycomb Repressive Complex 1 (PRC1) with tri-methylated H3K27 is sufficient to fold the methylated chromatin. Unexpectedly, such chromatin folding leads to spatial clustering of the DNA elements bound by PRC1. Our results provide further insight into mechanisms of epigenetic repression and the process of chromatin folding in response to histone methylation. American Society for Biochemistry and Molecular Biology 2023-07-25 /pmc/articles/PMC10470199/ /pubmed/37499944 http://dx.doi.org/10.1016/j.jbc.2023.105080 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Article
Lizana, Ludvig
Nahali, Negar
Schwartz, Yuri B.
Polycomb proteins translate histone methylation to chromatin folding
title Polycomb proteins translate histone methylation to chromatin folding
title_full Polycomb proteins translate histone methylation to chromatin folding
title_fullStr Polycomb proteins translate histone methylation to chromatin folding
title_full_unstemmed Polycomb proteins translate histone methylation to chromatin folding
title_short Polycomb proteins translate histone methylation to chromatin folding
title_sort polycomb proteins translate histone methylation to chromatin folding
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10470199/
https://www.ncbi.nlm.nih.gov/pubmed/37499944
http://dx.doi.org/10.1016/j.jbc.2023.105080
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