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DnaQ mediates directional spacer acquisition in the CRISPR-Cas system by a time-dependent mechanism
In the spacer acquisition stage of CRISPR-Cas immunity, spacer orientation and protospacer adjacent motif (PAM) removal are two prerequisites for functional spacer integration. Cas4 has been implicated in both processing the prespacer and determining the spacer orientation. In Cas4-lacking systems,...
Autores principales: | Tang, Dongmei, Jia, Tingting, Luo, Yongbo, Mou, Biqin, Cheng, Jie, Qi, Shiqian, Yao, Shaohua, Su, Zhaoming, Yu, Yamei, Chen, Qiang |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10470216/ https://www.ncbi.nlm.nih.gov/pubmed/37663930 http://dx.doi.org/10.1016/j.xinn.2023.100495 |
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